Method for breeding minitype potato seeds by directly using potato stem segments

Abstract

The invention provides a production method of minitype potato seeds bred by directly using potato stem segments, relating to the minitype potato seed high power propagation technology. In the invention, potato detoxification test tube tissue culture plantlet stem segments are used as a raw material, and then are directly implanted in an MS liquid or solid induction medium without any plant growth hormone, and the minitype potato seeds capable of sprouting are induced and produced in 6-8 weeks. The tissue culture plantlet stem segments are directly bred without changing the medium, thus obtaining the potato seeds in one step. The method has the advantages of saving time, labor and cost, having simple equipment, less used plantlets, less consumed time, rapid propagation and high efficiency and the like. The breeding period is 1.5-2 months, production can be performed for 5-6 periods each year, and the breeding chamber at unit area can produce 500,000-600,000 minitype potato seeds, thus making full use of the tissue culture chamber production space, greatly improving the output of the detoxification minitype potato seeds at each unit area, and increasing the production efficiency at each unit area by 50-100 times. The method is suitable for large-scale factory production of minitype potato seeds.

Description

technical field [0001] The invention belongs to the field of plant cloning and bioengineering technology. technical background [0002] The production of virus-free seed potatoes is a key link in the potato seed breeding system, and the production of virus-free micro-tubers is the core and basis for the breeding of high-quality seed potatoes at all levels. Test-tube microtubers are small tubers produced in the laboratory. If they can be used directly, the net room and field propagation will be completely omitted, and the problem of virus re-infection will be avoided. Therefore, it is a difficult problem that the large-scale industrial production of potato seed potatoes in the world expects to overcome. At the same time, the application of virus-free seed potatoes is the key to solving the degradation of seed potatoes in potato production and improving the efficiency of potato production. [0003] The traditional virus-free seed potato production is based on the production o...

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Problems solved by technology

[0007] The purpose of the present invention is to provide a method for directly cultivating miniature seed potatoes from potato stem segments, to establish a simple and efficient laboratory production procedure for miniature seed potatoes, and to provide a method with simple structure, excellent performance and easy operation. , The box-type culture container that can be placed on the culture rack can be overlapped to solve the problem that the tissue-cultured seedlings in the existing potato seed breeding method must be transplanted to the greenhouse and the net room. Too many seedlings are used, and the experimental space cannot be fully utilized. Virus-free Problems in the harvesting and picking of small potatoes are inconvenient

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