Method for biosynthesizing abietane diterpenoids by culturing cells from cloverleaf

A technique for rosinane diterpenes and culturing cells, which is applied in the field of inducing the biosynthesis of rosin alkane diterpenoids by liquid suspension culture cells of Tricuspid chinensis, can solve the problems of difficulty in obtaining, restricting research and application, low content and the like, and achieves improved purification. Efficiency, cost reduction, easy separation and purification
CN102295545AInactive Publication Date: 2011-12-28DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
Publication Date
2011-12-28
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a plant cell culture technology, in particular to a method for biosynthesizing abietane diterpenoid compounds by using cephalotaxus fortune culture cells. The method comprises the following steps of: placing cephalotaxus fortune cells in a common culture medium, and culturing, wherein50 to 150g of the cells are inoculated in each liter of wet weight of the culture medium; and adding an inducer at the final concentration of 10 to 2,000 mu M and 50 to 200g of adsorbent into each liter of culture medium at the initial stage of cell exponential growth, wherein the inducer is an abiological inducer or fungal elicitor. The method is a biosynthesis method and is stable and environment-friendly; and products are completely adsorbed onto the adsorbent, so that the operation process of separation and purification is greatly simplified, the cost is reduced and industrial production is easy to realize. Meanwhile, seven abietane diterpenoid compounds comprising a new compound are synthesized. The abietane diterpenoid compounds have high bioactivity, and are important lead compounds, and the technology has important commercial application prospect.
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Description

technical field

[0001] The invention relates to a plant cell culture technique, in particular to a method for inducing a liquid suspension culture cell of the cloverleaf to biosynthesize an abietane diterpenoid compound, and a new compound is included in the product at the same time. Background technique

[0002] Abietin diterpenoids belong to tricyclic diterpenoids and are an important class of natural compounds. The main reason why this kind of compounds attract people's research interest is that many substances in them have good activity, and many folk medicines contain such substances. For example, hinokiol (compound 2) in the present invention has inhibitory effects on Staphylococcus aureus, Streptococcus, Escherichia coli, Pseudomonas aeruginosa, etc., and is a good antibacterial lead compound. However, these compounds are mainly extracted from plants at present, and there are few reports on their chemical synthesis, and they are still in the stage of laboratory resea...

Claims

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