Microbial compositions for the prevention or reduction of growth of fungal pathogens on plants

A composition, microbial technology, applied in the direction of plant growth regulators, chemicals for biological control, fungi, etc., capable of solving problems that do not show plant or fungal specificity or effectiveness, etc.

Active Publication Date: 2020-10-30
博思特生物群落公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Commercially available products may not demonstrate desired plant or fungal specificity or effective...

Method used

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  • Microbial compositions for the prevention or reduction of growth of fungal pathogens on plants
  • Microbial compositions for the prevention or reduction of growth of fungal pathogens on plants
  • Microbial compositions for the prevention or reduction of growth of fungal pathogens on plants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0158] Example 1: Screening of microorganisms for antifungal activity

[0159] Screening of microorganisms for their ability to prevent the growth of the fungal diseases Verticillium dahliae and Fusarium oxysporum. Fourteen candidate microorganisms were identified as active in a primary screen that would form clearing zones on the fungal lawn (for example, see image 3 , on the identification of Hansenula vinespora for inhibition of Fusarium oxysporum). These fourteen candidate microorganisms were then screened in a secondary test designed to simulate a structured soil environment by using semi-solid agar. In this test, the growth of untreated fungi was set as 100% and the reduction relative to the growth of untreated fungi was determined. Serenade, a commercial product containing the active ingredient of the Bacillus subtilis strain QST713, was used as a control, which reduced the growth of Fusarium oxysporum by -25% and by >99% of the growth of Verticillium dahliae. Of th...

Embodiment 2

[0163] Example 2: Antifungal activity of Bacillus amyloliquefaciens (strain 28B; BC8) supernatant against raspberries

[0164] The antifungal activity of culture supernatants of an isolated strain of Bacillus amylocii (strain 28B; BC8) was determined against Botrytis cinerea on raspberries. After 120 hours, raspberries treated with the supernatant of the B. amyloliquefaciens strain showed similar fungal growth to the negative control raspberries (not infected with Botrytis cinerea), covering less than 5% of the surface area of ​​the raspberries. In contrast, after 120 hours, positive control raspberries (which had been infected with Botrytis cinerea) showed about 90% of their surface area covered by fungal growth ( Figure 4 ; Figure 5A-Figure 5C ).

Embodiment 3

[0165] Example 3: Evaluation of the efficacy of BC8 against blight caused by Botrytis cinerea and A. aureus anthracnose on blueberry crops.

[0166] The efficacy of BC8 was evaluated against flower rot caused by Botrytis cinerea and A. aureus anthracnose in blueberry crops. Blueberry bushes were treated with BC8 or a control treatment. As control treatments, shrubs were left untreated or treated with Lifegard (Certis; active ingredient: Bacillus mycoides), Stargus (Marrone; active ingredient: Bacillus amyloliquefaciens strain F727) and Nufilm (Fertrell, terpene polymer and emulsifier), or a combination of BravoWeatherstik (Syngenta; active ingredient: chlorothalonil (tetrachloroisophthalonitrile) 54%), Captevate (Arysta; active ingredient: fenhexamid, captan) and Treatment was carried out with continuous application of Pristine (BASF; active ingredients: pyraclostrobin, bosclid). All treatments also receive standard commercial fertility and pesticide programs. Treatment pro...

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Abstract

Disclosed herein are biocontrol compositions against plant fungal pathogens and methods of use thereof for the prevention or reduction of crop loss or food spoilage. The biocontrol composition can comprise at least one microbe with anti-fungal activity or a secondary metabolite of the at least one microbe. The methods can comprise application of the biocontrol composition to a plant, a seed, or aproduce thereof or to a packaging material used to transport or store the produce.

Description

[0001] cross reference [0002] This application claims priority to US Provisional Application No. 62 / 629,525, filed February 12, 2018, which is incorporated herein by reference in its entirety. Background technique [0003] Fungal pathogens cause significant agricultural losses, resulting in crop loss, food waste, and economic loss. Microorganisms with antifungal properties have been developed as biocontrol agents to reduce crop loss and food spoilage caused by these fungal pathogens. Commercially available products may not exhibit the desired plant or fungal specificity or effectiveness. In addition, limited options exist for postharvest conservation of produce, especially organic produce. Biocontrol compositions that prevent fungal growth could provide an alternative to currently available products. Contents of the invention [0004] In certain embodiments, described herein is a biocontrol composition comprising: (i) at least one microorganism, and (ii) a carrier; whe...

Claims

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Application Information

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IPC IPC(8): A01G13/00A23B7/16A23B9/14A01N43/40A01N63/32A01N63/27A01N63/22A01N63/20A01P3/00A01P5/00A01P7/02A01N63/50
CPCA01N63/20A01N63/22A01N63/27A01N63/32A01H3/00A01N63/50C12N1/20C12N1/16A01P3/00A01N37/46A01N43/72A01N43/713A01C1/06A01C21/00
Inventor 罗伯特·麦克布莱德凯伦·亨特杰米·巴彻维罗妮卡·加西亚
Owner 博思特生物群落公司
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