Method for increasing pulullan yield

A pullulan polysaccharide and yield technology is applied in the stepwise addition of growth factors to increase the yield of pullulan polysaccharide and the field of pullulan polysaccharide production, which can solve the disadvantages of large-scale, large-scale production, high cost of pullulan polysaccharide, high cost of pullulan polysaccharide, Problems such as low substrate utilization, to achieve the effect of shortening the fermentation cycle, convenient operation, and improving the conversion rate

Inactive Publication Date: 2014-04-02
天津北洋百川生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still problems of long fermentation cycle, low substrate conversion rate and low substrate utilizat...

Method used

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  • Method for increasing pulullan yield
  • Method for increasing pulullan yield
  • Method for increasing pulullan yield

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] (1) Seed cultivation

[0047] After activating the strain of Aureobasidium pullulans, pick one ring and transfer it to a 500ml baffle bottle, the volume of the culture medium is 100ml, the culture temperature is 32°C, the rotation speed of the shaker is 180rpm, and the culture time is 28h.

[0048] (2) Fermentation culture

[0049] The 500mL baffle bottle has a liquid volume of 100mL, an inoculum size of 4% (V / V), a temperature of 28°C, and a shaker speed of 400rpm for cultivation. When the growth of the bacteria is in the early logarithmic phase, add 0.03‰ vitamin B 1 , when the growth of the bacteria was in the early stage of the stationary phase, 0.04‰ of uracil nucleotides was added to accumulate the secondary metabolite, that is, pullulan.

[0050] (3) Separation of pullulan

[0051] After filtering out the bacterial cells in the fermentation broth, add three times of ethanol, stir, stand overnight at 4°C, centrifuge, rinse twice with absolute ethanol, and dry t...

Embodiment 2

[0054] (1) Seed cultivation

[0055] After activating the strain of Aureobasidium pullulans, pick one ring and transfer it to a 500ml baffle bottle, the volume of the culture medium is 100ml, the culture temperature is 32°C, the rotation speed of the shaker is 180rpm, and the culture time is 30h.

[0056] (2) Fermentation culture

[0057] The 500mL baffle bottle has a liquid volume of 100mL, an inoculum size of 4% (V / V), a temperature of 28°C, and a shaker speed of 400rpm for cultivation. When the growth of the bacteria is in the early logarithmic phase, add 0.04‰ vitamin B 1 , when the growth of the bacteria was in the early stage of the stationary phase, 0.03‰ of uracil nucleotides was added to accumulate the secondary metabolite, pullulan.

[0058] (3) Separation of pullulan

[0059] After filtering out the bacterial cells in the fermentation broth, add three times of ethanol, stir, stand overnight at 4°C, centrifuge, rinse twice with absolute ethanol, and dry to obtain ...

Embodiment 3

[0062] (1) Seed cultivation

[0063] After activating the strain of Aureobasidium pullulans, pick one ring and transfer it to a 500ml baffle bottle with a medium volume of 100ml, a culture temperature of 32°C, a shaker speed of 180rpm, and a culture time of 32 hours.

[0064] (2) Fermentation culture

[0065] A 500 mL baffle bottle has a liquid volume of 100 mL, an inoculum size of 4% (V / V), a temperature of 28° C., and a shaker rotation speed of 400 rpm for cultivation. When the bacterial growth is in the early logarithmic phase, add 0.05‰ vitamin B 1 , when the growth of the bacteria was in the early stage of the stationary phase, 0.02‰ of uracil nucleotides was added to accumulate the secondary metabolite, that is, pullulan.

[0066] (3) Separation of pullulan

[0067] After filtering out the bacterial cells in the fermentation broth, add three times of ethanol, stir, stand overnight at 4°C, centrifuge, rinse twice with absolute ethanol, and dry to obtain 75.6 g / L of whi...

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Abstract

The invention discloses a method for increasing pulullan yield by sub-sectional adding of growth factors in a fermentation process, and belongs to the technical field of biological fermentation engineering. The method comprises the following steps: producing pulullan from aureobasidium pullulans CGMCC NO.7055 in a fermentation manner; adding 0.003-0.005% of vitamin B1 when bacteria growth is at the beginning of the logarithmic phase; and adding 0.002-0.004% of uridine monophosphate when the bacteria growth is at the early stage of a stable stage, so as to induce and facilitate accumulation of secondary metabolite, namely the pulullan. The growth factors are specifically added according to the characteristics of sugar production in different growth periods and fermentation processes, so that the method is convenient and fast to operate and has an obvious effect; the fermentation period is greatly shortened, the transformation rate of a substrate is increased, and the cost of the pulullan is reduced.

Description

Technical field: [0001] The invention relates to a method for producing pullulan polysaccharide by microbial fermentation, in particular to a method for increasing the yield of pullulan polysaccharide by adding growth factors in stages during the fermentation process. The invention belongs to the technical field of biological fermentation engineering. Background technique: [0002] Pullulan is a water-soluble amorphous glucan and as a model polysaccharide of water-soluble polysaccharides, it has been used as an emulsifier, suspending agent, thickener, stabilizer, gelling agent, film-forming agent and lubricant It is widely used in food, pharmaceutical, petroleum, chemical and other fields. Pullulan polysaccharide will be an important development direction of new fermentation engineering in the future, and it has been paid more and more attention by domestic enterprises. [0003] Growth factors refer to the nutrients that are necessary for the growth of microorganisms and m...

Claims

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Application Information

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IPC IPC(8): C12P19/10C12R1/645
Inventor 乔长晟王建梓宋亚琼郝华旋
Owner 天津北洋百川生物技术有限公司
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