3 results about "Uridine monophosphate" patented technology

The invention provides a method for catalytically producing uridine monophosphate and application, and belongs to the technical field of genetic engineering and microbial engineering. The uridine kinase CaUDK-F gene with the nucleotide sequence as shown in SEQ ID NO.1 is adopted, the uridine kinase CaUDK-F gene is overexpressed through escherichia coli and then combined with polyphosphate kinase PPK to conduct biological catalysis on a substrate uridine, uridylic acid can be efficiently produced, the concentration of uridylic acid in a catalysis system is high, separation and purification are convenient, and the technology is simple. Meanwhile, the uridine kinase CaUDK-F is subjected to site-specific mutagenesis by adopting a site-specific mutagenesis technology, and three key amino acid residue sites S85, L87 and E88 which influence enzyme activity are subjected to site-specific mutagenesis, so that the catalytic efficiency and the conversion rate of the uridine kinase are further improved; wherein the enzyme activity of the mutant S85A is increased to 2.6 times that of a wild enzyme, and when the mutant S85A catalyzes uridine to produce uridylic acid, the conversion rate is increased to 92% from 80% of the wild enzyme.

The application provides a uridine monophosphate specific glycoside hydrolase and application thereof in biosynthetic synthesis of pseudouridine. Nmygdh The function of the gene is verified by in-vitro enzyme activity experiment Nmygdh The recombinant protein has broad-spectrum and high-efficiency catalytic activity, and can efficiently catalyze the hydrolysis of glycosidic bonds in various nucleotides, such as uridine monophosphate (UMP), guanosine monophosphate (GMP), adenosine monophosphate (AMP) and cytidine monophosphate (CMP), and the activity of uridine monophosphate is the strongest. The application uses the protein to efficiently hydrolyze uridine monophosphate to obtain two substrates of pseudouridine, i.e. uracil and 5'-phosphoribose, and realizes the atom-economical and efficient biosynthetic synthesis of pseudouridine by combining the cascade reaction of pseudouridine glycosidase EcPsuG and dephosphorylase EcYjjG.

This invention provides a kit for the simultaneous detection of nine flavor nucleotides in livestock and poultry products. The flavor nucleotides are cytidine monophosphate, uridine monophosphate, guanylic acid, inosine monophosphate, adenosine monophosphate, hypoxanthine, inosine, adenosine diphosphate, and adenosine triphosphate. The kit includes: a high-concentration extraction reagent, a standard curve solution, a high-concentration phosphate buffer solution, pH adjustment solution A, and pH adjustment solution B. The high-concentration extraction reagent is perchloric acid; the standard curve solution is a mixed solution of the nine flavor nucleotides; the high-concentration phosphate buffer solution is a solution of potassium dihydrogen phosphate and dipotassium hydrogen phosphate; pH adjustment solution A is sodium hydroxide; and pH adjustment solution B is phosphate. This invention also provides a method for the simultaneous detection of the nine flavor nucleotides using the above kit. This invention enables the simultaneous determination of the content of nine flavor nucleotides in livestock and poultry products. The method is simple, rapid, accurate, and sensitive, and can be used for batch determination, showing good prospects for practical application.