This invention provides a kit for the simultaneous detection of nine
flavor nucleotides in
livestock and poultry products. The
flavor nucleotides are
cytidine monophosphate,
uridine monophosphate, guanylic acid,
inosine monophosphate,
adenosine monophosphate,
hypoxanthine,
inosine,
adenosine diphosphate, and
adenosine triphosphate. The kit includes: a high-concentration extraction
reagent, a
standard curve solution, a high-concentration
phosphate buffer solution, pH adjustment solution A, and pH adjustment solution B. The high-concentration extraction
reagent is
perchloric acid; the
standard curve solution is a
mixed solution of the nine
flavor nucleotides; the high-concentration
phosphate buffer solution is a solution of
potassium dihydrogen
phosphate and
dipotassium hydrogen phosphate; pH adjustment solution A is
sodium hydroxide; and pH adjustment solution B is phosphate. This invention also provides a method for the simultaneous detection of the nine flavor nucleotides using the above kit. This invention enables the simultaneous determination of the content of nine flavor nucleotides in
livestock and poultry products. The method is simple, rapid, accurate, and sensitive, and can be used for batch determination, showing good prospects for practical application.