76 results about "Uridylic Acids" patented technology

The invention discloses a new method for synthesizing uridylic acid disodium. The method comprises the following steps: adding cytidylic acid and sodium nitrite in deionized water, and dropwise adding inorganic or organic acid at the constant temperature of minus 5 DEG C-0 DEG C; stirring the solution and heating to 10-60 DGE C, reacting for 1-12 hours, and introducing steam for steam distillation after the reaction is finished so as to obtain a uridylic acid solution; regulating the pH of the uridylic acid solution to 7.0-8.5 with alkali, distilling at reduced pressure until a liquid is not distilled out; adding 95% ethanol the weight of which is 3-12 times that of cytidylic acid, heating and refluxing for 0.5-6 hours under the condition of stirring, crystallizing, and filtering so as to obtain crude uridylic acid disodium; and dissolving crude uridylic acid disodium in a proper amount of water, recrystallizing with ethanol the weight of which is 1-3.5 times that of the crude uridylic acid disodium, filtering and drying so as to obtain the uridylic acid disodium product. By using the method, the yield of uridylic acid disodium is above 92.75%, the purity of uridylic acid disodium by detection of high performance liquid chromatography (HPLC) is more than 99.50%, and the purity of uridylic acid disodium by detection of ultraviolet (UV) light is above 98.50%. The method has the advantages of simple and reasonable process, good product quality stability, low production cost and the like, and is easy to be applied to massively production.

The invention relates to an enzyme preparation for preparing uridylic acid and a method for preparing the uridylic acid through enzyme catalysis. The method comprises the following steps: taking a material liquid containing uridine as a substrate, adding an escherichia coli cell crushing liquid with activity of uridine kinase and poly-phosphokinase, sodium hexametaphosphate, magnesium sulfate anda small amount of ATP (Adenosintriphosphat), and carrying out an enzymatic reaction under conditions that the pH value is 8.0 and the temperature is 30 DEG C to synthesize the uridylic acid. In a coupling catalysis reaction system, the uridine and the ATP are catalyzed by the uridine kinase to generate the uridylic acid, and ADP (Adenosine Diphosphate) is formed along with the dephosphorylation ofthe ATP. Poly-phosphokinase is in charge of catalyzing the sodium hexametaphosphate and the ADP to generate the ATP, so that the regeneration of the ATP is achieved in reactions. The uridylic acid production method provided by the invention has the advantages of being low in raw material price, short in period, simple and convenient to operate, green and environmentally-friendly and high in yield, and has good industrial application value.

The invention discloses a broiler chicken feedstuff additive. The feedstuff additive comprises the following components in parts by weight: 12-33 parts of adenylate, 5-24 parts of guanylic acid, 8-27 parts of uridylic acid, 14-39 parts of cytidine monophosphate, 5-22 parts of glutamine and 6-14 parts of arginine. The invention also discloses a preparation method and application of the broiler chicken feedstuff additive. According to the feedstuff additive disclosed by the invention, the appetite of animals can be boosted, the animals can be promoted to take food actively, the nutrient demand of young chickens in a best immune state is satisfied, the disease resistance and the disease prevention capability of the young chickens are enhanced, and the survival rate of the young chickens obviously increases, so that the feedstuff additive has a positive significance to a livestock culture industry.

The invention discloses a screening method and an application of uridine monophosphate modified protein in mitochondria. The method is characterized in that biotin-labeled UTP (Biotin-16-UTP) is usedas a raw material, under the action of uridine monophosphate transferase SelO, total mitochondrial protein is subjected to UMP modification reaction, and the protein modified by UMP further has a biotin label, subsequently, the modified protein is screened out by using a biotin-streptavidin Pull-down technology, and finally, the modified protein and a modification site are determined by mass spectrometry. The method is advantaged in that a simple and feasible experimental method is developed, proteins which can be modified by UMP are screened from mitochondrial total proteins, and an importantresearch means is provided for signal transduction mediated by SelO proteins and post-translational modification in mitochondria.

The invention discloses a production method of a flos traditional Chinese medicine. The production method is characterized in that nutrients are used during the growth of the flos traditional Chinese medicine, wherein the nutrients include the following components in percentage by weight: 30 to 70% of nucleotide salt and 30 to 70% of medium trace element, and exclude glucose or / and amino acids; the nucleotide salt is the sylvite or sodium salt of four 5'-mixed nucleotides and obtained by hydrolyzing ribonucleic acid alkaline, namely, wherein the four 5'-mixed nucleotides are namely 5'-adenylate dipotassium or sodium, 5'-guanylic dipotassium or sodium, 5'-cytidine monophosphate dipotassium or sodium, 5'-uridylic acid dipotassium or sodium; and the medium trace element includes Mn, B and Fe, namely manganese sulfate, borax and ferrous sulfate. The production method of the flos traditional Chinese medicine has the effect reaching and even exceeding the effect of a patent for invention with publication patent number of CN102079666B and patent number of ZL201010514231.6. The method is economic and simple. The obtained flos traditional Chinese medicine is large and uniform in flower, the uniformity is up to 70 to 90%, the output is 20 to 40% higher than that of flos traditional Chinese medicine produced by the traditional method, the main components account for 10 to 25%, the obtained traditional Chinese medicines can be prepared into quality decoction pieces, or active ingredients can be extracted, and the yield is up to 10 to 22%.