18 results about "Cytidylic Acids" patented technology

The invention belongs to the technical field of biological medicine, and particularly discloses a composite vaccine adjuvant as well as a preparation method and application thereof. The composite vaccine adjuvant provided by the invention is an oil-in-water emulsion, and simultaneously contains squalene, polysorbate 80, sorbitan trioleate, polyinosinic acid (Poly I: C) and CpG oligodeoxynucleotide (CpG-ODN). The sequence-optimized CpG-ODN and Poly I: C are used in a specific emulsion system in a combined mode, a remarkable synergistic immune enhancement effect can be generated, the induced humoral immunity and cellular immunity response level is remarkably higher than the sum of the effects when all the components are independently used, and the composite adjuvant is good in stability, high in immunostimulatory activity and suitable for clinical application. The immune effect can be remarkably improved by combined application with various vaccine antigens. The invention also provides a preparation method and application of the composite vaccine adjuvant.

This invention relates to a zebrafish model, its establishment method, and its applications, relating to the biomedical field. The establishment method includes the following steps: injecting zebrafish with polyinosinic-polycytidylic acid (poly I:C), resuscitating, and culturing. This method can obtain a stable zebrafish model that can simulate an inflammatory state similar to that in humans following viral infection. This model can be used to assess whether antiviral drugs inhibit viral activity and the differences in efficacy among different antiviral drugs, thereby enabling preliminary screening of antiviral drugs.

The application discloses a transient transfection method for improving recombinant protein expression of CHO cells, and relates to the technical fields of eukaryotic cell gene transfection and animal cell culture. A cell suspension is obtained by mixing a CHO cell and a serum-free CHO cell basic culture medium; DNA and a transfection reagent are added into the cell suspension to mix a transfection culture, 5-azadeoxycytidine acid solution is added into the transfection culture at 0h-24h to form a transfection system and continue the transfection culture; the transfection system is added with a feeding culture medium at 1d-4d after the transfection culture to carry out suspension culture; the suspension culture is centrifuged at 7d to separate and harvest cell culture supernatant to complete recombinant protein expression. According to the application, 5-azadeoxycytidine acid is added into the transfection culture at a specific concentration, so that the expression of recombinant protein of the CHO cell is significantly improved during the transfection expression.

The present disclosure relates to the technical field of medicine, and in particular to a pharmaceutical composition, preparation method and use thereof. The pharmaceutical composition includes a first active ingredient, a second active ingredient, and a pharmaceutically acceptable carrier or excipient. The first active ingredient is a microbial agent, including one or more of Staphylococcus aureus, Bordetella pertussis, diphtheria toxoid, tetanus toxoid, Salmonella typhi, and Salmonella paratyphi. The second active ingredient includes polyinosinic acid, polycytidylic acid, and vitamin. The pharmaceutical composition of the present disclosure pertains to an artificial active immunotherapy for tumors. It can “stimulate” the entire immune system, making the therapy of using bacteria to activate the human immune system to kill cancer cells highly stable and reliable. This composition can significantly save and prolong the lives of cancer patients while exhibiting extremely high safety, minimal toxic side effects, and low production costs.

This skin barrier function improving agent contains cytidylic acid or a precursor thereof as an active ingredient.

The invention discloses a method for chiral separation of tryptophan by using a capillary silica gel monolithic column modified by gold nanoflowers coated with cytidine monophosphate, and belongs to the field of pharmaceutical analysis. The method comprises the following steps: firstly synthesizing cytidine monophosphate coated gold nanoflower, using the cytidine monophosphate coated gold nanoflower as a chiral selector to prepare a capillary monolithic column modified by the cytidine monophosphate coated gold nanoflower, and separating chiral molecules under an electrophoresis condition. And the baseline separation of the tryptophan enantiomer can be realized. The separation method is simple to operate, and a novel high-selectivity chiral separation detection technology is established.

This invention provides a kit for the simultaneous detection of nine flavor nucleotides in livestock and poultry products. The flavor nucleotides are cytidine monophosphate, uridine monophosphate, guanylic acid, inosine monophosphate, adenosine monophosphate, hypoxanthine, inosine, adenosine diphosphate, and adenosine triphosphate. The kit includes: a high-concentration extraction reagent, a standard curve solution, a high-concentration phosphate buffer solution, pH adjustment solution A, and pH adjustment solution B. The high-concentration extraction reagent is perchloric acid; the standard curve solution is a mixed solution of the nine flavor nucleotides; the high-concentration phosphate buffer solution is a solution of potassium dihydrogen phosphate and dipotassium hydrogen phosphate; pH adjustment solution A is sodium hydroxide; and pH adjustment solution B is phosphate. This invention also provides a method for the simultaneous detection of the nine flavor nucleotides using the above kit. This invention enables the simultaneous determination of the content of nine flavor nucleotides in livestock and poultry products. The method is simple, rapid, accurate, and sensitive, and can be used for batch determination, showing good prospects for practical application.

The application discloses a kind of solid phase extraction-liquid quality connection simultaneously detecting multiple nucleotides in drinking water in the technical field of water pollution monitoring, first using solid phase extraction enrichment target material, nitrogen blow concentration, finally using the concentration of guanosine acid, adenosine acid, cytidine acid, uridine acid, deoxyguanosine acid, deoxyadenosine acid, deoxycytidine acid and deoxythymidine acid is determined using the multiple reaction monitoring mode of liquid chromatography tandem mass spectrometry, the present application combines solid phase extraction pretreatment technology with the detection technology of liquid chromatography tandem mass spectrometry, realizes the detection of trace nucleotide under complex matrix condition, the present application can realize the simultaneous determination of multiple nucleotides by one sample injection, with the advantages of high recovery rate, short detection time, many detection types and the like.

The present invention discloses a pharmaceutical composition, its manufacturing method, and its use, wherein the pharmaceutical composition comprises a first active ingredient, a second active ingredient, and a pharmaceutically acceptable carrier or excipient, the first active ingredient being a microbial agent containing one or more of Staphylococcus aureus, Bordetella pertussis, diphtheria toxoid, tetanus toxoid, Salmonella typhi, or Salmonella paratyphi, and the second active ingredient comprising polyinosinic acid, polycytidylic acid, and vitamins. The pharmaceutical composition is an artificial active immunotherapy for tumors, which, by "stimulating" the entire immune system, activates the human immune system using bacteria to kill cancer cells, making the treatment extremely stable and reliable, significantly saving and extending the lives of cancer patients, while simultaneously being extremely safe, having very few toxic side effects, and having low manufacturing costs.

The invention provides application of taurochenodeoxycholic acid and / or chenodeoxycholic acid and polyinosinic acid combined medicine in preparation of medicine for preventing and / or treating melanoma, and belongs to the field of biological medicine. It is found for the first time that taurochenodeoxycholic acid and / or chenodeoxycholic acid can influence the tumor immune microenvironment by regulating and controlling the antigen presentation function of cDC1, and the treatment effect of polyinosinic-cytidylic acid is improved. The melanoma model experiment directly shows that the combination of chenodeoxycholic acid and polyinosinic-cytidylic acid can better inhibit tumor growth, can effectively promote CD8 + T cell infiltration in tumors, has more cDC1 infiltration in tumor tissues, shows a stronger inhibition effect on tumors, and can be used for preparing drugs for treating tumors. The pharmaceutical composition plays a synergistic role in treatment of melanoma, and provides a new choice for clinical treatment of melanoma.

The invention relates to a preparation method of uridine 5 '-disodium phosphate and uridine 5'-disodium phosphate. The uridine 5 '-disodium phosphate comprises the following raw materials: cytidine 5'-phosphoric acid; the catalytic enzyme is cytidine monophosphate deaminase; cytidine 5 '-phosphate is used as a raw material, an elastin tag is fused behind an initiation codon of cytidine deaminase to obtain cytidine deaminase with the elastin tag, the cytidine deaminase with the elastin tag is purified, and uridine 5'-disodium phosphate is synthesized through a one-step catalytic reaction. The uridine 5 '-disodium phosphate prepared by the preparation method disclosed by the invention does not contain a large amount of phosphate, magnesium ions and adenylic acid impurities, so that a large amount of sewage generated in the purification process is avoided.

The present disclosure relates to lipid nanoparticle (LNP) compositions including at least one ionizable cationic lipid and Polyinosinic:polycytidylic acid (poly(I:C)), and compositions and formulations incorporating such LNP compositions. The disclosure also provides methods of using these compositions to elicit an immune response, including for the prevention or treatment of infectious diseases.

The application discloses a method for enhancing 5'-cytidylic acid accumulation and application, and belongs to the field of genetic engineering and microbial engineering. The application knocks out ppnN, ushA, yrfG, yjjG, umpH and umpG genes, overexpresses 5'-CTP diphosphohydrolase gene nudG and orotidine-5'-phosphate ribosyltransferase gene pyrE, integrates expression of uridine phosphorylase mutant gene PyrH(R92G / D93G), further knocks out cytidine deaminase gene cdd and ribonucleoside-triphosphate reductase gene nrdD, and integrates expression of uridine-cytidine kinase gene udk, phosphoribosyl pyrophosphate synthetase gene prs, glucose-6-phosphate dehydrogenase gene zwf and 6-phosphogluconate dehydrogenase gene, so that the 5'-cytidylic acid accumulation capacity of Escherichia coli is improved. Under the condition that no antibiotic and inducer are added, the 5'-cytidylic acid yield of the recombinant bacteria reaches 39.6 g / L in a 60L fermenter.

The present application relates to a kind of preparation, separation detection method and application of the impurity of sodium cytidine phosphate with structure I, comprising the following steps: 5'-cytidylic acid, choline phosphate and sodium tert-butanolate aqueous solution are mixed to be prepared into reaction solution, then catalyst is added to carry out reaction, the solution after reaction is collected, chromatographic column chromatography separation, crystallization drying, the impurity of sodium cytidine phosphate with structure I is obtained.The raw material of the preparation method of the present application is environmental protection, process is simple, yield is high, fills the technical blank of the preparation of the impurity of sodium cytidine phosphate with structure I, and the research on the preparation method is conducive to guiding the optimization of cytidine phosphate sodium synthesis process route and ensuring cytidine phosphate sodium safe drug use.

The invention provides a yeast hydrolysate as well as a preparation method and application thereof, the yeast hydrolysate comprises the following components in percentage by mass: 35-65% of true protein, aromatic amino acid and sulfur-containing amino acid, and the content ratio of the aromatic amino acid to the sulfur-containing amino acid is 2-5; the ratio of the content of the components with the weight-average molecular weight of 200-2000Da to the content of the components with the weight-average molecular weight of more than 2000Da is 4-10, and the ratio of the content of the components with the weight-average molecular weight of 200-2000Da to the content of the components with the weight-average molecular weight of less than 200Da is 4-9; the yeast hydrolysate further comprises disodium nucleotide, the disodium nucleotide comprises disodium inosinate, disodium guanylate, disodium cytidine monophosphate and disodium uridinemonophosphate, and the ratio of the mass sum of the disodium inosinate and the disodium guanylate to the mass sum of the disodium cytidine monophosphate and the disodium uridinemonophosphate to the disodium nucleotide is 1: (0.6-1.2): (1.6-2.2). The feed additive is beneficial for further improving the feed intake of the piglets and reducing the disease incidence of the piglets.

The invention discloses an engineering strain for efficiently producing cytidine monophosphate and application, and relates to the technical field of engineering strains and application, the host of the strain is Escherichia coli BL21 (DE3), the strain knockout ppnP gene and ppnN gene at the same time through a CRISPRCas9 double gRNA system, so that the degradation activity of endogenous nucleoside and nucleotide is reduced, and the double gRNA system respectively targets two different sites in open reading frames of ppnP and ppnN. According to the engineering strain for efficiently producing cytidine monophosphate and the application, ppnP and ppnN genes in an escherichia coli genome are targeted and knocked out at the same time by utilizing a CRISPR-Cas9 double gRNA system, and the activity of endogenous nucleotide degrading enzyme of a host strain is remarkably reduced, so that the stability of cytidine and cytidine monophosphate is effectively improved. The engineering strain after gene knockout and modification shows that the synthesis efficiency of 5 '-cytidine monophosphate is remarkably improved, the generation of byproducts is remarkably reduced, and the purity and yield of the product are both superior to those of a product obtained by a traditional method.

The present invention relates to compositions comprising particles, each of said particles comprising a complex of at least one double-stranded polyribonucleotide, such as polyinosinic-polycytidylic acid [poly(IC)], and at least one linear polyal-kyleneimine. The particles are also characterized by their monomodal diameter distribution and z-average diameter within specific ranges. The present invention additionally relates to the use of said compositions as medicaments, in particular for the treatment of a cell growth disorder characterized by abnormal growth of human or animal cells, as well as to processes for the preparation of said compositions.

The invention discloses deoxycytidine monophosphate hydroxylase AfhB and application of the deoxycytidine monophosphate hydroxylase AfhB. The dCMP hydroxylase AfhB obtained by screening from the genome of the bacteriophage HY126 can be used for green synthesis or fermentation production of 5hdCMP, so that the environmental pollution caused by a traditional chemical synthesis method is greatly reduced, and a new enzyme element and way are provided for development of a gene editing technology and fermentation production of 5hdCMP and derivatives thereof in industrial fermentation.