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Mixed nucleotide which comes from yeast and contains uridylic acid and preparing method and application thereof

A technology of mixing nucleotides and uridine acid, which is applied in applications, animal feed, animal husbandry, etc., can solve problems affecting animal growth and production efficiency, piglet weaning syndrome, piglet gastrointestinal dysfunction, etc., and achieve the goal of feeding Significant feeding effect, reduced wastage rate, and reduced diarrhea rate

Active Publication Date: 2016-11-23
ANGELYEAST CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Early weaning can easily cause gastrointestinal dysfunction in piglets and induce piglet weaning syndrome, which greatly affects the growth and production efficiency of animals

Method used

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  • Mixed nucleotide which comes from yeast and contains uridylic acid and preparing method and application thereof
  • Mixed nucleotide which comes from yeast and contains uridylic acid and preparing method and application thereof
  • Mixed nucleotide which comes from yeast and contains uridylic acid and preparing method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Prepare yeast-derived mixed nucleotides containing uridine acid and yeast hydrolyzate. The preparation method is: use ribonucleic acid as raw material, enzymatically hydrolyze to obtain primary yeast hydrolyzate containing mixed nucleotides, and detect the mixed nucleotides On the basis of the content of each component, the pure mononucleotide is added to obtain the mixed nucleotide containing uridine acid and yeast hydrolyzate. The preparation method of the mixed nucleotide and yeast hydrolyzate of the present invention will be further illustrated through Examples 1-1 to 1-10 below.

Embodiment 1-1

[0042] Preparation of mixed nucleotides containing uridine acid and yeast hydrolyzate derived from yeast, the steps are as follows:

[0043] Add yeast ribonucleic acid into water to prepare 10wt% base material, add 2000u / gRNA phosphodiesterase, adjust pH to 5.5, carry out enzymatic hydrolysis at 65°C, heat up to 95°C after 6h, maintain for 15 minutes, stop enzymatic reaction. Spray-dry the enzymatic solution to obtain the primary yeast hydrolyzate containing AMP, CMP, GMP, and UMP, and add the five mononucleotide pure products, so that the final modified yeast hydrolyzate is tested to reach the contents of various substances: 1.8% mixed nucleotides, 46.4% crude protein, 39.4% total amino acids, 1.14% crude fat, 7% ash, 6% moisture, of which , the weight ratio of the five nucleotides in the mixed nucleotides in the yeast hydrolyzate is: cytidylic acid: adenylic acid: guanylic acid: uridylic acid: inosinic acid=43:116:95:800: 1.

[0044] Wherein, the detection method of above...

Embodiment 1-2

[0058] Preparation of mixed nucleotides containing uridine acid and yeast hydrolyzate derived from yeast, the steps are as follows:

[0059] According to the method described in Example 1-1, adjust the pH to 5.3 and the temperature to 60°C to prepare a primary yeast hydrolyzate containing AMP, CMP, GMP, UMP, add cytidylic acid, adenylic acid, guanylic acid, urine Inosinic acid and inosinic acid are five kinds of pure mononucleotides, so that the final modified yeast hydrolyzate is tested to reach the content of each substance: mixed nucleotides 1.5%, crude protein 45%, total amino acids 38%, Crude fat 1.2%, ash 7.5%, moisture 8%. Wherein, the weight ratio of the five nucleotides in the mixed nucleotides in the yeast hydrolyzate is: cytidylic acid: adenylic acid: guanylic acid: uridylic acid: inosinic acid = 43:154:95:800 :1.

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PUM

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Abstract

The invention relates to the industrial field of animal feed, in particular to mixed nucleotide which comes from yeast and contains uridylic acid and a preparing method and application thereof. The mixed nucleotide contains, by weight, 43-80 parts of cytidine monophosphate, 116-200 parts of adenylate, 95-150 parts of guanylic acid, 400-800 parts of uridylic acid and 1 part of inosinic acid. The invention further provides a yeast autolysate containing the mixed nucleotide, wherein the weight percentage of the mixed nucleotide in the yeast autolysate is 1.5-2%. By the adoption of the yeast autolysate, the growing speed of piglets can be effectively increased, productivity and feed conversion rate are high, average daily gain of piglets is increased by 9.8-17.4%, and application prospects are broad.

Description

technical field [0001] The invention relates to the field of animal feed industry, in particular to a mixed nucleotide rich in uridine acid and yeast hydrolyzate specially used for piglets, in particular to a mixed nucleotide containing uridine acid derived from yeast and its preparation method and apply. Background technique [0002] Weaning stress has always been a difficult problem in the pig industry, and it is also one of the propositions to be solved urgently in the field of animal nutrition. Early weaning is likely to cause gastrointestinal dysfunction in piglets and induce piglet weaning syndrome, which affects the growth and production efficiency of animals to a large extent. During stress, piglets have a limited ability to synthesize nucleotides in their intestines, and dietary nucleotide deficiency may be one of the factors leading to gastrointestinal dysfunction. Yeast hydrolyzate (AY) is rich in nucleotides, and nucleotides have positive effects on the morphol...

Claims

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Application Information

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IPC IPC(8): A23K1/00A23K1/14A23K1/08A23K1/06A23K1/18
Inventor 吴信俞学锋李知洪姚鹃李彪
Owner ANGELYEAST CO LTD
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