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Nucleotide mixture crystalline powder and preparation method thereof

A crystal powder and mixture technology, which is applied in the field of nucleotide mixture crystal powder and its preparation, can solve the problems of poor feed economy, high separation cost, and long production cycle, and achieve low cost, high purity, and good growth. Effect

Active Publication Date: 2018-02-23
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method has low-temperature chromatography and ion exchange operations, and the separation cost is high. At the same time, each monomer separated by low-temperature chromatography needs to be concentrated and crystallized by nanofiltration. The operation is cumbersome and the production cycle is long. It is economical to use in the feed industry. poor

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  • Nucleotide mixture crystalline powder and preparation method thereof
  • Nucleotide mixture crystalline powder and preparation method thereof

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Effect test

Embodiment 1

[0046] Take 400g of RNA enzymatic hydrolysis solution, its pH is 4.4, the concentration of uridine is 7.0g / L, the concentration of guanosine is 6.5g / L, the concentration of cytidine is 5.0g / L, and the concentration of adenylate is 4.8 g / L, after plate and frame filtration, add 0.5 g of activated carbon to the filtrate, stir at room temperature for 60 min, filter, and discard the filter residue. Under stirring conditions at 40°C, the pH of the filtrate was adjusted to 6.0-7.5 with 1 mol / L sodium hydroxide solution, then it was concentrated 4 times, and 2 times the volume of 95 vol.% ethanol was added to it, while adding 2 The temperature was lowered to 20°C at a rate of ℃ / h, and the crystals were transformed for 4 hours. Then, the crystal slurry was filtered, and the filter cake layer was washed with 1 volume of 90% ethanol. Nucleotide mixture of uridylic acid, guanylic acid, cytidylic acid and adenylic acid, the crystallization yield is 82%, the product liquid phase purity is ...

Embodiment 2

[0048] Take 400g of RNA enzymatic hydrolysis solution, its pH is 4.5, the concentration of uridine is 10.0g / L, the concentration of guanosine is 9.1g / L, the concentration of cytidine is 7.50g / L, and the concentration of adenylate is 7.8 g / L, after plate and frame filtration, add 1 g of activated carbon to the filtrate, stir at 50 °C for 40 min, filter, and discard the filter residue. Under stirring conditions at 35°C, the pH of the filtrate was adjusted to 7.5-8.5 with 1.5 mol / L sodium hydroxide solution, then it was concentrated 5.5 times, and 4 times the volume of 80 vol. Cool down to 15°C at a rate of 1°C / h, transform into crystallization for 3 hours, filter the crystal slurry, and use 1 volume of 95% ethanol to wash the filter cake layer. Nucleotide mixture of uridine, guanylate, cytidine and adenylate, 90% crystallization yield, 98.5% liquid phase purity of the product, uridine, guanylate, cytidine and adenylate The mass ratio of 1.8:1.5:1.2:1.0.

Embodiment 3

[0050] Take 500g of RNA enzymatic hydrolysis solution, its pH is 4.7, the concentration of uridine is 4.8g / L, the concentration of guanosine is 4.5g / L, the concentration of cytidine is 3.8g / L, and the concentration of adenylate is 3.0 g / L, after plate and frame filtration, add 2g activated carbon to the filtrate, stir at 40°C for 30min, filter, discard the filter residue, heat up to 50°C, equilibrate under stirring for 30min, and then use 2mol / L sodium hydroxide solution to remove The pH of the filtrate was adjusted to 8.5-10.0, then it was concentrated 7 times, and 3 times the volume of anhydrous ethanol was added to it, and at the same time, the temperature was lowered to 15°C at a rate of 3°C / h, and the crystallization was transformed for 3 hours. The solution was filtered and used The filter cake layer was washed with 1 volume of anhydrous ethanol, and the obtained solid was air-dried at 35°C to obtain a nucleotide mixture containing uridylic acid, guanylic acid, cytidine a...

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Abstract

The invention discloses nucleotide mixture crystalline powder and a preparation method thereof. The preparation method comprises the following steps of performing solid-liquid separation on ribonucleic acid enzymatic hydrolysate, decoloring the enzymatic hydrolysate, regulating the pH of decolored enzymatic hydrolysate to 6.5-10.0, performing concentration, performing crystallization, performing filtration, and performing drying so as to obtain the nucleotide mixture crystalline powder containing uridylic acid, guanylic acid, cytidylic acid and adenylic acid. The nucleotide mixture crystallinepowder provided by the invention is high in purity, good in stability and free from moisture absorption, has favorable granularity and mobility, and can be widely applied to feed trade. The preparation method provided by the invention is high in yield, simple to operate and low in cost, and is suitable for industrial production.

Description

technical field [0001] The invention belongs to the field of animal feed industry, in particular to a nucleotide mixture crystal powder and a preparation method thereof. Background technique [0002] As an important low-molecular-weight compound in living organisms, nucleotides play important physiological and biochemical functions in the body. They can synthesize genetic material, transmit cell signals, participate in energy metabolism, and act as coenzymes. Cell proliferation in animals requires nucleotides to synthesize nucleic acids, especially in rapidly dividing lymphoid and intestinal tissues. The source of nucleotides can be de novo synthesis pathway and salvage pathway, animals can synthesize nucleotides in vivo, and salvage pathway is to use exogenous nucleotides or nucleotide fragments to synthesize new nucleotides. Since animals can synthesize nucleotides, they have not been regarded as essential nutrients for a long time. However, research results in recent yea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K50/30A23K20/153
CPCA23K20/153A23K50/30
Inventor 应汉杰杨朋朋黎青青王森吴菁岚柳东朱晨杰陈勇欧阳平凯
Owner NANJING UNIV OF TECH
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