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Nucleotide mixture powder and preparation method and application thereof

A technology of mixtures and nucleotides, applied in the fields of application, animal feed, additional food elements, etc., can solve the problem of low enzymolysis rate, achieve high enzymolysis rate, reduce diarrhea rate and promote mortality, and have good granularity Effect

Active Publication Date: 2019-11-19
NANJING BIOTOGETHER
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the existing reports, nucleotides are all prepared by one-pot method, that is, the nuclease enzyme solution is added to the ribonucleic acid solution at one time for enzymatic hydrolysis reaction, which will use a large amount of enzyme solution, and the enzymatic hydrolysis rate is not high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Take 150g ribonucleic acid powder and dissolve it in water, set the volume to 3L, adjust the pH to 5.5, and gradually add nuclease enzyme solution (enzyme activity 2000U / mL) with a pump. The nuclease solution was placed in a 5L reactor with a volume of 150mL, and the jacket was kept at 70°C. The feeding time was controlled for 6 hours, and the enzymatic hydrolysis was continued for 5 hours after the feeding was completed. Concentrate to 300 mL with a rotary evaporator, and spray dry at 150°C to obtain a nucleotide mixture powder. The enzymolysis rate of this method is 75%, the nucleotide yield is 95.5%, the nucleotide content is 82%, the particle diameter d50 is 95 μm, and the disodium uridine, guanylate disodium, cytidylic acid and adenylic acid The mass ratio is 0.9:1.5:0.6:1.

Embodiment 2

[0030] Take 100g ribonucleic acid powder and dissolve it in water, set the volume to 3L, adjust the pH to 5.0, and gradually add nuclease enzyme solution (enzyme activity 2000U / mL) with a pump. The nuclease solution was placed in a 5L reactor with a volume of 250mL, and the jacket was kept at 70°C. The feeding time was controlled for 4 hours, and the enzymatic hydrolysis was continued for 5 hours after the feeding was completed. Concentrate to 300 mL with a rotary evaporator, and spray dry at 150°C to obtain a nucleotide mixture powder. The enzymolysis rate of this method is 71%, the nucleotide yield is 93%, the nucleotide content is 78%, the particle size d50 is 88 μm, disodium uridine, disodium guanylate, cytidylic acid and adenylic acid The mass ratio is 0.7:1.7:0.6:1.

Embodiment 3

[0032] Take 250g ribonucleic acid powder and dissolve it in water, set the volume to 3L, adjust the pH to 6.3, and gradually add nuclease enzyme solution (enzyme activity 2000U / mL) with a pump. The nuclease solution was placed in a 5L reactor with a volume of 350mL, and the jacket was kept at 70°C. The feeding time was controlled for 7 hours, and the enzymatic hydrolysis was continued for 5 hours after the feeding was completed. Concentrate to 400 mL with a rotary evaporator, and spray dry at 150°C to obtain a nucleotide mixture powder. The enzymolysis rate of this method is 68%, the yield of nucleotides is 92.8%, the nucleotide content is 75%, the particle size d50 is 80 μm, disodium urylate, disodium guanylate, cytidylic acid and adenylic acid The mass ratio is 0.7:1.8:0.5:1.

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PUM

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Abstract

The invention discloses nucleotide mixture powder and a preparation method and application thereof. The preparation method comprises the following steps of adding a ribonucleic acid solution in nuclease liquid in a fed-batch manner, performing enzymolysis, performing concentration, and performing drying so as to obtain the nucleotide mixture powder containing uridylic acid, guanylic acid, cytidylic acid and adenylic acid. The nucleotide mixture powder provided by the invention is high in purity and good in stability, is favorable in fluidity, and can be widely applied to feed trade. The preparation method provided by the invention is high in yield, simple to operate, low in cost, and suitable for industrial production.

Description

technical field [0001] The invention relates to a nucleotide mixture powder and its preparation method and application, belonging to the field of animal feed industry. Background technique [0002] As an important low-molecular compound in organisms, nucleotides play important physiological and biochemical functions in the organism. It can synthesize genetic material, transmit cell signals, participate in energy metabolism, and act as a coenzyme. Cell proliferation in animals requires nucleotides to synthesize nucleic acids, especially in rapidly dividing tissues such as lymphoid and intestinal tissues. The source of nucleotides can be the de novo synthesis pathway and the salvage pathway, in which animals can synthesize nucleotides in vivo, and the salvage pathway is the synthesis of new nucleotides using exogenous nucleotides or nucleotide fragments. Because animals can synthesize nucleotides themselves, people have not regarded them as essential nutrients for a long time...

Claims

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Application Information

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IPC IPC(8): C12P19/30C12P19/32A23K50/30A23K20/153
CPCA23K20/153A23K50/30C12P19/305C12P19/32
Inventor 陈晓春张磊汤亦文齐昌瑞
Owner NANJING BIOTOGETHER
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