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Method for evaluating destroy degree on nucleotide of determination method and production technology of free nucleic acid hydrolysate in protein product

A technology of free nucleic acid and determination method, which can be used in measurement devices, material separation, instruments, etc., and can solve problems such as animal toxicity, yeast product quality decline, and nucleotide content reduction.

Active Publication Date: 2015-04-15
江苏征泰饲料有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This will result in a decrease in the quality of the yeast product, and a reduction in the amount of nucleotides provided, which in turn can reduce the immune function of the animal. At the same time, the increased bases such as adenine will cause toxicity to animals.

Method used

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  • Method for evaluating destroy degree on nucleotide of determination method and production technology of free nucleic acid hydrolysate in protein product
  • Method for evaluating destroy degree on nucleotide of determination method and production technology of free nucleic acid hydrolysate in protein product
  • Method for evaluating destroy degree on nucleotide of determination method and production technology of free nucleic acid hydrolysate in protein product

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Embodiment Construction

[0030] The following are the implementation steps of the present invention:

[0031] 1 Reagents and solutions

[0032] 1.1 Unless otherwise specified, the reagents used in this method are all analytically pure, and the water is deionized water, which meets the requirements of GB / T 6682 secondary water.

[0033] 1.2 Potassium dihydrogen phosphate: analytically pure.

[0034] 1.3 Potassium hydroxide: analytically pure.

[0035] 1.4 Methanol: chromatographically pure.

[0036] 1.5 Acetonitrile: chromatographically pure.

[0037] 1.6 20% w / v potassium hydroxide solution: Weigh 100 grams of potassium hydroxide, dissolve it in 500ml of water, and store it in a volumetric flask.

[0038] 1.7 Mobile phase A: 0.1M potassium dihydrogen phosphate solution, weigh 27.2 grams of potassium dihydrogen phosphate, dissolve in 1000ml of water, add 1.0ml of 20% potassium hydroxide solution, add water to make the volume to 2 liters. The 0.45um water system filter membrane is used for use after filtration.

[0...

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Abstract

The invention discloses a method for determining a free nucleic acid hydrolysate in protein products. The method comprise the following steps: (1) respectively preparing a nucleotide mixed standard solution and a bases mixed standard solution; (2) preparing a solution of a sample to be tested, and using the nucleotide mixed standard solution and the base mixed standard solution described in step 1 as the reference solution, and employing high performance liquid chromatography through washing peak area method comparison to obtain the content of free nucleotides and free base in the sample. According to the invention, high performance liquid chromatography is used for determining 5 kinds of bases in a single-cell protein raw material (cytosine, uracil, guanine, thymine and adenine), 5 kinds of nucleotides (cytidine monophosphate, uridylic acid, guanylic acid, inosinic acid and adenylic acid), and 5 kinds of nucleosides (cytidine, uridine, inosine, guanosine and adenosine); and then the destroy degree on nucleotide of the production process is determined according to t the relationships between the bases and nucleotides, and between the bases and nucleosides.

Description

Technical field [0001] The invention belongs to the technical field of nucleic acid hydrolysate determination, especially the field of single-cell protein production quality control. Background technique [0002] Single cell protein (SCP), also known as microbial protein or bacterial protein, uses industrial wastewater, waste gas, natural gas, petroleum alkanes, agricultural and sideline processed products, and organic waste as a medium to cultivate yeast and non-pathogenic Single-celled organisms such as bacteria, micro-bacteria, and fungi are then purified and dried and are made into an important source of protein in the food industry and feed industry. [0003] Single-cell protein products are rich in nucleotides, and yeast products on the market are the main nucleotide providers in the feed industry. Due to the characteristics of the process, products such as autolyzed yeast, enzymatic yeast, and hydrolyzed yeast contain more free nucleic acid hydrolysates. Studies have found ...

Claims

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Application Information

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IPC IPC(8): G01N30/02
Inventor 许建刚
Owner 江苏征泰饲料有限公司
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