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Partially reduced graphene oxide, preparation method and application thereof

A graphene and graphene technology, applied in the field of partially reduced graphene oxide and its preparation, can solve the problems of laborious screening of aggregation crystallization and cross-linking conditions, difficult to become the preferred catalyst for bioprocess engineers, and limited reactor configuration.

Active Publication Date: 2019-04-12
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Immobilized enzymes can be divided into carrier and carrier-free immobilized enzymes. Although the development of carrier-free enzymes such as CLEA or CELC does not require the use of additional carriers that occupy the main body but have no catalytic activity, carrier-free immobilized enzymes have inherent defects. : Restricted reactor configurations and laborious screening of aggregate crystallization and cross-linking conditions make them difficult to be the catalyst of choice for bioprocess engineers
Because porous supports have a large surface area, but large surface area is not a sufficient condition for high loading

Method used

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  • Partially reduced graphene oxide, preparation method and application thereof
  • Partially reduced graphene oxide, preparation method and application thereof
  • Partially reduced graphene oxide, preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0043] Embodiment 1: the preparation of carrier

[0044] 20 mg of graphene oxide powder was dissolved in 40 ml of pure water, and it was sufficiently dispersed by ultrasound to prepare a 0.5 g / L graphene oxide dispersion. Take the graphene oxide dispersion (0.5g / L), add 20mg of disodium guanylate, fully dissolve, and react in a magnetically stirred water bath at 25°C for 6h, and finally centrifuge, wash, and freeze-dry to obtain the desired carrier GMPNa 2 -GO. The graphene oxide dispersion was taken for centrifugation and freeze-drying. Take equal amounts of graphene oxide and GMPNa 2 -GO solid, ground into powder, and carried out XPS analysis, confirmed that GO was modified, such as figure 1 .

Embodiment 2

[0045] Embodiment 2: the influence of reducing agent dosage on GO

[0046] 20 mg of graphene oxide powder was dissolved in 40 ml of pure water, and it was sufficiently dispersed by ultrasound to prepare a 0.5 g / L graphene oxide dispersion. Take the graphene oxide dispersion (0.5g / L), add 20mg and 80mg disodium guanylate respectively, fully dissolve, and react in a magnetically stirred water bath at 25°C for 6h, and finally centrifuge, wash, and freeze-dry to obtain Required carrier GMPNa 2 -GO. The graphene oxide dispersion was reacted in a magnetically stirred water bath at 25°C for 6 hours, and finally centrifuged and freeze-dried. Take equal amounts of graphene oxide and GMPNa 2 -GO solid, ground into powder, and carried out SEM, TEM analysis, confirmed that GO was modified, such as figure 2 , image 3 .

Embodiment 3

[0047] Example 3: The effect of the reduction degree of the carrier on the activity of the immobilized enzyme.

[0048] 20 mg of graphene oxide powder was dissolved in 40 ml of pure water, and it was sufficiently dispersed by ultrasound to prepare a 0.5 g / L graphene oxide dispersion. Take the graphene oxide dispersion (0.5g / L), add 20mg, 40mg, and 80mg of disodium guanylate respectively, fully dissolve, and react in a magnetically stirred water bath at 25°C for 6h, and finally perform centrifugation, washing, and freeze-drying , to obtain carriers with different degrees of reduction (GMPNa 2 -0.5g / L-GO, GMPNa 2 -1g / L-GO, GMPNa 2 -2g / L-GO). The graphene oxide dispersion was taken for centrifugation and freeze-drying. Take the same amount of graphene oxide and different reduction degrees of GMPNa 2 -GO solid, ground into powder, and carried out XPS analysis, confirmed that GO was modified, such as Figure 4 .

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Abstract

The invention discloses a preparation method of partially reduced graphene oxide, belonging to the application technical field of graphene oxide. Graphene oxide is dispersed in water, a reducing agentis added and reacted to obtain partially reduced graphene oxide; the reducing agent is one or more of components selected from adenylate, disodium adenylate, dipotassium adenylate, adenosine-5'-diphosphate dipotassium salt, cytidylic acid, cytidine-5'-monohposphate disodium salt, cytidine dipotassium, guanylic acid, disodium guanylate, dipotassium guanylate, uridylic acid, disodium uridine-5'-monophosphate, and dipotassium uridine-5'-monophosphate. Nucleotides have different types of R groups, and can interact with graphene oxide by various forces, such as hydrophobic force, electrostatic force and hydrogen bond force, so as to control the surface properties of graphene oxide.

Description

technical field [0001] The invention belongs to the technical field of graphene oxide application, and in particular relates to a partially reduced graphene oxide and its preparation method and application. Background technique [0002] Enzymes belong to the class of natural catalysts, including DNA, RNA, and catalytic antibodies. The unique function of enzymes is that all the reactions they catalyze can be carried out orderly, selectively and precisely under mild physiological conditions. Lipase belongs to a subclass of esterase, which can catalyze the hydrolysis, synthesis, peptide synthesis, biosurfactant synthesis, and transesterification of glyceride compounds. These characteristics make lipase useful in food, cosmetics, agriculture, papermaking, etc. field is widely used. However, the natural characteristics of lipase cannot meet the requirements of its catalytic reaction. Lipases usually have a hydrophilic shell and a lipophilic core, and when they catalyze lipophi...

Claims

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Application Information

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IPC IPC(8): C01B32/184C01B32/198C12N11/14
CPCC12N11/14C01B32/184C01B32/198C01B2204/04C01B2204/32
Inventor 应汉杰庄伟周文凤朱倩倩吴菁岚杨朋朋牛欢青柳东陈勇朱晨杰陈晓春
Owner NANJING UNIV OF TECH
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