6 results about "Uridine phosphorylase" patented technology

Methods of treating a subject for a liver condition, e.g., NAFLD, NASH, and / or DILI, are provided. Aspects of the methods include administering to the subject an effective amount of a UPase inhibitor, optionally in combination with a uridine active agent (e.g., uridine (UR), a UR pro-drug or a UR mimetic), such as supplemental uridine, to treat the subject for the liver condition. Also provided are compositions for use in practicing the subject methods.

The invention discloses an enzyme composition, an immobilized enzyme and application. The enzyme composition consists of uridine phosphorylase and thymine phosphorylase. The enzyme composition and the immobilized enzyme are simple to prepare, have the characteristics of high conversion efficiency, high yield and simplicity and convenience in operation when being used for synthesizing 2 '-fluoro-2'-deoxycytidine, and can effectively reduce the production cost. Meanwhile, synthetic reaction conditions of the 2 '-fluoro-2'-deoxycytidine based on the enzyme composition and the immobilized enzyme are mild, green and environment-friendly, and the environmental problem caused by chemical synthesis can be avoided.

The invention relates to the field of biochemical detection, and discloses a method for detecting the content of cytidine in fermentation liquor based on an enzyme method and high flux, and the method comprises the following steps: providing a first reagent containing uridine phosphorylase, dihydropyrimidine dehydrogenase, glucose-6-phosphate dehydrogenase and related coenzyme substrates, and a second reagent containing cytidine deaminase. During detection, a sample and a first reagent are mixed for background absorbance monitoring; then a second reagent is added to start a specific reaction, cytidine is catalyzed into uridine, the uridine consumes the reduced coenzyme through a cascade enzyme reaction, and meanwhile, the coenzyme is cyclically amplified by a regeneration system. The cytidine content is determined by monitoring the decline rate of the specific wavelength absorbance. By adopting a two-step sample adding method, the interference of a sample matrix is effectively deducted, and the detection accuracy is improved; by utilizing the design of coenzyme regeneration cycle, a detection signal is amplified, and the sensitivity is improved; by combining the automatic operation of the porous microplate, high-throughput detection is realized, and the efficiency is improved.

This invention provides a uracil-producing strain, its construction method, and its application. The strain utilizes CRIPSR / Cas9 gene editing technology to produce... E. coli On the UR14 genome, firstly by knocking out psuG Gene, black Gene, rutA Genes and upp Genes were introduced to block the breakdown of uracil; then, the uridine phosphorylase gene was overexpressed. udp and pyrimidine-5'-nucleotide nuclease gene ppnN This synergistically enhances the synthesis and accumulation of uracil; finally, overexpression of the phosphoribosyltransferase gene... pgm, Further enhancing the conversion of the byproduct 1-phosphate ribose into the precursor 5-phosphate ribose-1-pyrophosphate improves carbon utilization. The resulting strain exhibits good genetic stability and high fermentation yield, enabling stable production of uracil and demonstrating broad application prospects.

The invention describes a method of the enzymatic synthesis of cordycepin and its 2-halogen derivatives by reacting 3 -deoxyuridine with adenine or its 2-halogen derivatives in an aqueous phosphate reaction medium under the catalytic action of uridine phosphorylase (UP) and purine nucleoside phosphorylase (PNP). The synthetic method according to the invention is fast, technologically and energetically undemanding and environmentally friendly, and does not require the presence of organic solvents or chromatographic purification of pure cordycepin, while being easily transferable to laboratory and mass production conditions.

The invention describes a method of the enzymatic synthesis of cordycepin and its 2-halogen derivatives by reacting 3 -deoxyuridine with adenine or its 2-halogen derivatives in an aqueous phosphate reaction medium under the catalytic action of uridine phosphorylase (UP) and purine nucleoside phosphorylase (PNP). The synthetic method according to the invention is fast, technologically and energetically undemanding and environmentally friendly, and does not require the presence of organic solvents or chromatographic purification of pure cordycepin, while being easily transferable to laboratory and mass production conditions.