Fermentation method for producing epothilone B by sorangium cellulosum and fermentation medium

A technology of C. celluliformis and a fermentation medium, which is applied to the field of fermentation and fermentation medium for the production of Epothilone B by C. cellulosum, and can solve the problems of difficulty in culturing, low yield, and mutagenesis screening of Myxobacter celluliformis Difficulty and other problems, to achieve the effect of stable strain performance, increased yield and significant effect
CN101851591AInactive Publication Date: 2010-10-06SHANGHAI INST OF PHARMA IND
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
SHANGHAI INST OF PHARMA IND
Publication Date
2010-10-06
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a sorangium cellulosum fermentation medium, which comprises a carbon source, a nitrogen source, inorganic salts, a macroporous absorption resin, and water. The fermentation medium also comprises amino acid. When the fermentation medium is used for preparing the epothilone B by fermentation culture of sorangium cellulosum, the performance of strains is stable, the growth is rapid, and the yield of the product epothilone B is obviously improved. The invention also provides a fermentation method for producing the epothilone B by the fermentation culture of the sorangium cellulosum by using the sorangium cellulosum fermentation medium.
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Description

technical field

[0001] The invention relates to a fermentation method and a fermentation medium for producing epothilone B by S. cellulosus. Background technique

[0002] Epothilone B, as a secondary metabolite of the myxobacterium S. cellulosus, is a new type of anti-tumor active substance with ultra-stable microtubules. The mechanism of action of epothilone B is the same as that of paclitaxel, but it is superior to paclitaxel in terms of anti-tumor spectrum, anti-tumor activity, safety, water solubility and synthesis method. It is a new type of anti-tumor drug with broad prospects.

[0003] For the large-scale production of epothilone, biological fermentation is an ideal way. Epothilone B is generally produced by fermentation of the myxobacterium Sorangium cellulosum 90. It is reported in patent WO93 / 10121 that a low-yield epothilone B can be produced by cultivating Sonocystis cellulosus strains in a medium containing carbon sources, nitrogen sources and inorganic salts,...

Claims

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