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Method for starting livestock and poultry manure compost secondary fermentation by utilizing oyster mushrooms

A livestock and poultry manure, secondary fermentation technology, applied in the application, fertilization device, organic fertilizer and other directions, can solve problems such as poor adaptability of high nitrogen substrates, and achieve the effect of low environmental safety risk

Active Publication Date: 2020-06-12
GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Edible fungi are relatively safe fungi that also have the ability to ferment straw to produce fulvic acid. However, due to their poor adaptability to high-nitrogen substrates, it is difficult to directly apply them to compost fermentation.

Method used

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  • Method for starting livestock and poultry manure compost secondary fermentation by utilizing oyster mushrooms
  • Method for starting livestock and poultry manure compost secondary fermentation by utilizing oyster mushrooms

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1: start-up test of inoculating Pleurotus ostreatus mycelium plaque

[0029] Get 10g of high-temperature sterilized chicken manure primary compost product (equivalent to the fermentation product in the high-temperature stage, with a moisture content of 50%), and spread 1g of high-temperature sterilized corn stalk debris on the primary compost product to form corn stalk debris Upper and lower layers of compost; cut 0.5cm × 0.5cm Pleurotus ostreatus mycelium plaques from the solid PDB medium (the blank control does not add Pleurotus ostreatus mycelium plaques) and add them to the middle of the corn stalk debris, Then 50 μL of sterile water was added around the mycelium plaque, and the system was fermented at a constant temperature of 25° C. for 15 days. The one without Pleurotus ostreatus mycelium plaque was used as the blank control. Determination of fulvic acid content to judge the effect of secondary fermentation.

[0030] The results showed that the conte...

Embodiment 2

[0031] Embodiment 2: the growth form of the stalk of pre-inoculated Pleurotus ostreatus during interlayer fermentation

[0032] Get 2.5g chicken manure primary compost product (about 1cm thick, moisture content 50%), on the primary compost product, tile the corn stalk debris (about 0.5cm thick) of 0.5g pre-inoculation oyster mushroom, on the corn stalk debris Spread 2.5g of chicken manure primary compost products (about 1cm thick) on the top to form a sandwich pattern with corn stalk debris in the middle and compost on the top and bottom ( figure 1 ), fermented at a constant temperature of 25°C for 15 days. Observe the growth status of the mycelium of Pleurotus ostreatus in the straw layer. The corn stalk debris of the pre-inoculated Pleurotus ostreatus is to inoculate 1 mL of Pleurotus ostreatus spherical mycelium particles into 10 g of corn stalk crumbs under aseptic conditions, and pre-cultivate at 25°C for 10 days to obtain the pre-inoculated Pleurotus ostreatus of corn ...

Embodiment 3

[0034] Example 3: Comparison of different inoculation patterns (mixing, layering up and down, sandwiching)

[0035] (1) Hybrid mode:

[0036] Take 5g of chicken manure as a primary compost product (with a moisture content of 50%), add 0.5g of corn stalk debris, and mix evenly. Take 1.5mL of oyster mushroom spherical mycelium particles and evenly add them to the compost, and ferment at a constant temperature of 25°C for 15 days.

[0037] (2) Upper and lower layer mode:

[0038] Take 5g of chicken manure primary composting product (moisture content 50%), spread 0.5g of corn stalk debris on the primary composting product to form upper and lower layers with corn stalk debris on top and compost on the bottom, take 1.5mL oyster mushroom spherical The mycelium particles are evenly added to the corn stalk debris, and fermented at a constant temperature of 25° C. for 15 days.

[0039] (3) Mezzanine mode

[0040] Take 2.5g chicken manure primary compost product (moisture content 50%)...

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Abstract

The invention discloses a method for starting livestock and poultry manure compost secondary fermentation by utilizing oyster mushrooms. The method includes the steps of: inoculating pleurotus ostreatus mycelium into straw debris for pre-culture, and then spreading the pre-cultured straw debris on the primary fermentation product of the livestock and poultry manure to form a fermentation substrate; fermenting the fermentation substrate for the second time; or paving the straw fragments on the primary fermentation product of the livestock and poultry manure, and inoculating oyster mushroom mycelia on the straw fragments to obtain a fermentation substrate; and fermenting the fermentation substrate for the second time. The method has the beneficial effects: (1) straw cell walls are damaged and hemicellulose-cellulose is dissolved out after corn straw chippings are subjected to oyster mushroom pretreatment, so that a basis is provided for functional bacteria to convert hemicellulose-cellulose into fulvic acid after the functional bacteria are started; (2) the oyster mushrooms are pre-cultured, and the problem that the oyster mushrooms are difficult to grow in a high-nitrogen substrateis solved through an interlayer covering and adding means, so that the oyster mushrooms are smoothly colonized in a pile body;

Description

Technical field: [0001] The invention belongs to the field of comprehensive utilization of agricultural solid waste, and in particular relates to a method for starting secondary fermentation of livestock and poultry manure composting by using oyster mushrooms. Background technique: [0002] Aerobic composting is an effective measure for the resource utilization of livestock and poultry manure, which can realize the harmlessness, reduction and resource utilization of livestock and poultry manure, and is a key link in the realization of ecological cycle agriculture. Aerobic composting of livestock and poultry manure refers to the process of converting organic matter in livestock and poultry manure into fertilizer through the fermentation of microorganisms under artificial control (parameters such as water content, C / N and aeration degree). From an unstable state that is easy to degrade to a stable state of humus that is difficult to degrade and a certain form of nutrients such...

Claims

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Application Information

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IPC IPC(8): C05F15/00C05F17/20
CPCC05F3/00C05F11/00C05F11/08Y02W30/40
Inventor 杨旭楠刘丛竹张一帆许玫英
Owner GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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