Probiotic composition containing egg yolk antibody IgY and application preparation
A yolk antibody and probiotic technology, which is applied to bacteria and lactobacilli used in application and food preparation, can solve the problem of no combination and application of anti-Helicobacter pylori yolk antibody and probiotics, so as to improve the intestinal microenvironment. , the effect of regulating the intestines and improving the immunity of the body
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Embodiment 1
[0039] By weight, the formula of this embodiment
[0040]
[0041] Preparation:
[0042] 1. Raw material preparation
[0043] (1) Preparation of yolk antibody:
[0044] P1: Collect immunized eggs: use Helicobacter pylori-specific antigens to immunize laying hens, collect eggs after immunization, and keep them fresh;
[0045] P2: Egg yolk antibody extraction by water dilution method: Take the immunized eggs collected in step P1, remove the egg white under aseptic conditions to collect the yolk, add ultrapure water and magnetically stir, adjust the pH to 5.0~5.2, continue magnetic stirring, and place at -20℃~ Solidify overnight at -80°C. After completion, thaw at 4°C for 1 to 4 hours, then thaw at room temperature until completely thawed, collect the supernatant by centrifugation, and then filter through sterilized filter paper to collect the filtrate;
[0046] P3: Purification by sodium chloride salting-out method: add solid sodium chloride to the filtrate collected in st...
Embodiment 2
[0065] By weight, the formula of this embodiment
[0066]
[0067] All the other preparation methods are the same as in Example 1, and the specific steps are omitted here.
Embodiment 3
[0069] By weight, the formula of this embodiment
[0070]
[0071] All the other preparation methods are the same as in Example 1, and the specific steps are omitted here.
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