Composition for treating osteoarthritis as well as preparation method and application of composition

A technology for osteoarthritis and composition, applied in the field of composition, can solve the problem that the physiological state of cells cannot be fully realized, and achieve the effects of surface attachment, simple production process and convenient use

Active Publication Date: 2015-04-29
天晴干细胞股份有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, all these methods are carried out under the condition of the cell body outside, and the cell suspension is not deoxygenated when it is imported. When the cells are injected into the body, due to the complex inflammation and nutritional environment, the physiological state of the cells will be changed. Fully realize the experimental results obtained in vitro

Method used

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  • Composition for treating osteoarthritis as well as preparation method and application of composition
  • Composition for treating osteoarthritis as well as preparation method and application of composition
  • Composition for treating osteoarthritis as well as preparation method and application of composition

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specific Embodiment approach 1

[0027] Specific embodiment 1: The active ingredients of the composition for treating osteoarthritis in this embodiment include mesenchymal stem cells and cytokines, and the concentration of mesenchymal stem cells in the composition is 5×10 5 ~5×10 7 cells / mL, the cytokines are made from FGF2-rich plasma and VEGF-rich plasma at a volume ratio of 1:3-10.

[0028] The mesenchymal stem cells can be bone marrow mesenchymal stem cells, fat mesenchymal stem cells, umbilical cord mesenchymal stem cells, umbilical cord blood mesenchymal stem cells, amniotic membrane mesenchymal stem cells, placental mesenchymal stem cells, or endometrial sources mesenchymal stem cells.

[0029] Cytokine-rich plasma can regulate the arthritic microenvironment and reduce pain. At the same time, it also provides nutrients for the survival of co-transplanted mesenchymal stem cells, promotes their effective colonization and survival in vivo, and prolongs the regeneration and microenvironment of stem cells ...

specific Embodiment approach 2

[0030] Embodiment 2: This embodiment differs from Embodiment 1 in that: the concentration of FGF2 in the FGF2-rich plasma is 40-80 pg / mL. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0031] Embodiment 3: This embodiment differs from Embodiment 1 or Embodiment 2 in that: the VEGF concentration in the VEGF-rich plasma is 150-350 pg / mL. Others are the same as in the first or second embodiment.

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Abstract

The invention discloses a composition for treating osteoarthritis as well as a preparation method and an application of the composition, relates to a composition as well as a preparation method and an application thereof, and provides a composition for treating osteoarthritis and a preparation method and an application of the composition. The composition comprises the following effective components: mesenchymal stem cells and cytokine; and the cytokine is prepared from FGF2-rich plasma and VEGF-rich plasma. The method comprises the following steps: 1, preparing platelet-rich plasma; 2, preparing FGF2 factor-rich plasma; 3, preparing VEGF factor-rich plasma; and 4, mixing the FGF2 factor-rich plasma with the VEGF factor-rich plasma, balancing the mixed plasma under the oxygen partial pressure of 25-60mmHg, and adding the mesenchymal stem cells to obtain the composition for treating osteoarthritis. In the preparation process of the composition, an oxygen supply environment which is similar to the oxygen partial pressure in a human joint is adopted; the adaptive capacity of stem cells is promoted; and the composition plays a relatively good role in repairing osteoarthritis, and is used for preparing medicines for treating osteoarthritis.

Description

technical field [0001] The present invention relates to a composition, its preparation method and its application. Background technique [0002] Osteoarthritis (OA) is a common, multiple, and degenerative disease in the elderly, and its typical pathological features are destruction of articular cartilage, changes in subchondral bone structure, and occurrence of osteophytes. At present, among the elderly over 60 years old in my country, 55% of people suffer from osteoarthritis, and the prevalence rate increases year by year with age. As China gradually enters an aging society and the average life expectancy of Chinese people increases, about 120 million people are suffering from osteoarthritis. Due to poor local blood supply and bone marrow stem cell lesions, patients with osteoarthritis usually have limited self-regeneration and repair capabilities. Without therapeutic intervention, joint repair and functional recovery cannot be achieved. Drug therapy and surgery can only ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/28A61P19/02A61K35/16
CPCA61K35/16A61K35/28A61K38/1825A61K38/1866A61K2300/00
Inventor 张怡芦慧颖刘艳青
Owner 天晴干细胞股份有限公司
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