The invention belongs to the technical field of gene detection, and particularly relates to a low-frequency mutation detection method based on a snapback primer technology. The method comprises: designing a snapback primer, preparing a DNA template, preparing a detection system, setting a reaction procedure and determining a Ts value, judging a result, and other steps. According to the present invention, the detection method is the snapback ARMS and snapback primer HRM combined low-frequency mutation detection technology, such that the detection sensitivity of 1:10000 or even higher is achieved, clinical requirements of pollution resistance, high sensitivity, strong specificity, easy performing and result judgment on the mutation detection technology are met, and the method can be used for gene screening, genotyping (SNP, deletion mutation, insertion mutation) and other fields of various types of tissues including fresh tissue samples, whole blood, serum, tissue slices and other samples.