A method based on porous ceramic adsorption and immobilization of S. cellulosus fermentation
A technology of cellulosystis and porous ceramics, applied in the direction of microorganism-based methods, immobilized on or in inorganic carriers, fermentation, etc., can solve the problems of cell autolysis, unstable metabolic state, inconsistent state of internal and external cells, etc. , to achieve the effects of alleviating feedback inhibition, reducing production costs, and less wear and tear
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Embodiment 1
[0056] A method for fermenting and producing epothilone B based on porous ceramic adsorption and immobilization of S. cellulosus, comprising the following steps:
[0057] 1) Diatomite-based porous ceramics with a size of Ф20×10mm were sterilized at 150°C for 30 minutes at high temperature. After natural cooling, they were put into a conical flask and fermented liquid was added. After the liquid surface completely covered the porous ceramic samples, it was about 50mm, shake slowly at room temperature for 1 hour, let it stand for 2 hours, pour off the fermentation broth, and dry the porous ceramics at 105°C for later use.
[0058] 2) Inoculate a ring of fresh Sorangium cellulosum on M26 solid medium, and culture in a constant temperature incubator at 30°C for 72 hours;
[0059] Inoculate the cultured bacteria into 100ml of M26 liquid medium, culture at 30°C, 200rpm shaker for 72h;
[0060] After the cultivation, part of the liquid was discarded, and the remaining liquid was tra...
Embodiment 2
[0064] A method for fermenting and producing epothilone B based on porous ceramic adsorption and immobilization of S. cellulosus, comprising the following steps:
[0065] 1) Diatomite-based porous ceramics with a size of Ф20×10mm were sterilized at 150°C for 30 minutes at high temperature. After natural cooling, they were put into a conical flask and fermented liquid was added. After the liquid surface completely covered the porous ceramic samples, it was about 50mm, shake slowly at room temperature for 1 hour, let it stand for 2 hours, pour off the fermentation broth, and dry the porous ceramics at 105°C for later use.
[0066] 2) Prepare the bacterial suspension at a certain concentration with the method in Example 1.
[0067] 3) Take 5ml of the diluted bacterial suspension, put it into a 500mL culture bottle containing 100mL of fermentation broth and 1g of porous ceramics, and incubate on a shaker at 28°C and 180rpm for 96h.
Embodiment 3
[0069] A method for fermenting and producing epothilone B based on porous ceramic adsorption and immobilization of S. cellulosus, comprising the following steps:
[0070] 1) Diatomite-based porous ceramics with a size of Ф20×10mm were sterilized at 150°C for 30 minutes at high temperature. After natural cooling, they were put into a conical flask and fermented liquid was added. After the liquid surface completely covered the porous ceramic samples, it was about 50mm, shake slowly at room temperature for 1 hour, let it stand for 2 hours, pour off the fermentation broth, and dry the porous ceramics at 105°C for later use.
[0071] 2) Prepare a certain concentration of bacterial suspension with the method in Example 1.
[0072] 3) Take 10ml of the diluted bacterial suspension, put it into a 500mL culture bottle containing 100mL of fermentation broth and 1.5g of porous ceramics, and incubate on a shaking table at 30°C and 220rpm for 96h.
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