Method for preparing abalone viscera phospholipid through HPEF (High Pulsed Electric Field)-coupled biological enzymolysis

A technology of high-voltage pulsed electric field and abalone viscera, which is applied in the direction of edible phospholipid composition, food science, protein food composition, etc. Utilize problems such as abalone visceral residues to achieve good practicability and application prospects, easier control of production process conditions, and increase the effect of industrial added value

Active Publication Date: 2017-02-15
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The protein hydrolyzate prepared by this invention is rich in amino acids, rich in nutrition, and delicious in flavor, but only uses a single component in the abalone viscera, and fails to significantly increase the economic value of the abalone viscera
The invention patent with the publication number CN 103255186A "Co-production Preparation Method of Abalone Polysaccharides, Lipids and Protein Peptides" uses abalone viscera as raw material to extract and prepare abalone polysaccharides and lipids by means of complex enzymatic hydrolys

Method used

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  • Method for preparing abalone viscera phospholipid through HPEF (High Pulsed Electric Field)-coupled biological enzymolysis
  • Method for preparing abalone viscera phospholipid through HPEF (High Pulsed Electric Field)-coupled biological enzymolysis

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Embodiment 1

[0029] A method for preparing abalone visceral phospholipids by decoupling PEF and biological enzymes comprises the following steps:

[0030] 1) Principle pretreatment: Add 500 g of cleaned abalone viscera into distilled water at a solid-to-liquid weight ratio of 1:5, and beat it with a high-speed tissue masher at a speed of 10,000r / min;

[0031] 2) PEF and enzymatic hydrolysis: Put the twisted abalone viscera in the batching tank, and perform 17 min of high-voltage pulse electric field treatment, the electric field intensity is 30 KV / cm, the pulse time is 378 μS, and the pulse frequency is 296 Hz; then, it is treated with 0.2 M PBS Adjust the pH of the buffer solution to 6.8, add 3 g of compound protease at the ratio of AS.1398 neutral protease: papain: trypsin = 1:1:1, enzymolyze at 45°C for 2 h, and inactivate the enzyme at 90°C for 15 min; Centrifuge at 4000 rpm for 13 min to collect supernatant A2255 mL of abalone viscera extract, and dry the insoluble matter by hot air a...

Embodiment 2

[0037] A method for preparing abalone visceral phospholipids by decoupling PEF and biological enzymes comprises the following steps:

[0038] 1) Principle pretreatment: Add 500 g of cleaned abalone viscera to 4000 mL of distilled water, and beat it with a high-speed tissue grinder at a speed of 10000r / min;

[0039] 2) PEF and enzymatic hydrolysis: put the abalone viscera into a batching tank for 20 minutes of high-voltage pulse electric field treatment, the electric field intensity is 34 KV / cm, the pulse time is 450 μS, and the pulse frequency is 312 Hz; then, it is treated with 0.2 M PBS Adjust the pH of the buffer solution to 6.3, add 5 g of compound protease with the ratio of AS.1398 neutral protease: papain: trypsin = 1:0.5:0.8, enzymolyze at 45 °C for 2.5 h, and inactivate the enzyme at 90 °C for 15 min; Centrifuge at 4000 rpm for 15 min to collect 3180 mL of supernatant A of abalone viscera extract, and dry the insoluble matter by hot air at 65 ℃ to process 293 g of feed...

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Abstract

The invention relates to a method for preparing abalone viscera phospholipid through HPEF (High Pulsed Electric Field)-coupled biological enzymolysis and belongs to the field of deep processing of aquatic food. The method comprises the following preparation steps in sequence: raw material preprocessing, PEF-coupled biological enzymolysis, lipid extraction, phosphatide separation based on silicagel column chromatography, protein extraction, alcohol extraction of polysaccharide and the like, thereby sequentially obtaining phosphatide, neutral fat, protein, polysaccharide, pigment and the like in abalone. The method is simple in operating process and low in cost; the method is capable of performing deep processing and comprehensive utilization on byproducts-viscera generated by processing in the Chinese abalone industry, and serving as intermediate feed stock, the prepared phosphatide, protein, polysaccharide, pigment and the like of abalone can be applied to various fields such as foods and medicine. The technology is applicable to industrial production of various scales, and is capable of effectively solving waste discharge of the abalone production and processing industry.

Description

technical field [0001] The invention belongs to the field of intensive processing of aquatic food, and relates to the extraction of effective components of abalone viscera, in particular to a method for preparing abalone visceral phospholipids through high-voltage pulse electric field coupled biological enzymatic hydrolysis to obtain protein, polysaccharide and pigment at the same time. Background technique [0002] abalone( abalone ), also known as sole and sole, is a single-shell mollusk with high nutritional value. Every 1000 g of fresh abalone meat contains 350-500 g of protein, 240-380 g of carbohydrate, 80-220 g of lipid, and contains a variety of vitamins and trace elements. It is recorded in "Compendium of Materia Medica" that abalone is flat in nature, sweet and salty, and has the functions of improving eyesight and tonifying deficiency, clearing heat and nourishing yin, nourishing blood and stomach, and nourishing liver and kidney. It is a seaweed with high nutrit...

Claims

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Application Information

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IPC IPC(8): A23J7/00
CPCA23J7/00A23V2002/00A23V2250/2042
Inventor 陈继承赵晓丹池承灯陈艺晖陈莉
Owner FUJIAN AGRI & FORESTRY UNIV
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