Process for performing high pressure combined ultrasound-associated extraction on active substance from mushroom

Abstract

The invention provides a process for performing high pressure combined ultrasound-associated extraction on active substance from mushroom. The process sequentially comprises the following step: performing material preprocessing, pulverization, low-temperature lipid extraction, filter slag pressurization, ultrasound extraction and ethanol extraction, and finally drying to obtain the active substance. The method is characterized in that lipid extraction is adopted, the active substance dissolved with lipid is extracted, slag subjected to the lipid extraction adopts a high pressure combined ultrasound extraction technology to ensure that the difference between the internal pressure and the external pressure of the raw materials is changed to result in the change of a cell structure; then, an ultrasound-assisted extraction technology is adopted, an ultrasound cavatition function is utilized, target components are easily dissociated from an extracted matter, so that the active substance can be quickly and effectively extracted from the organism, thus greatly increasing the yield; then, the active substances subjected to lipid extraction and ethanol extraction are mixed and dried to prepare the active substance, and the active substance is stored at low temperature and the extraction rate of the active substance is greatly increased.

Description

technical field [0001] The invention relates to a method for extracting active substances in mushrooms, in particular to a process for extracting active substances in mushrooms assisted by high pressure combined with ultrasound, belonging to the technical field of biomedicine. Background technique [0002] At present, the research on the separation of antioxidants and anti-aging drugs from natural products has attracted widespread attention from scholars at home and abroad. Many research results have been achieved in this field. Based on research reports at home and abroad, the main active ingredients of natural antioxidants are It comes from plants, fungi (mushrooms), marine organisms, etc. Among them, the antioxidant active ingredients derived from plants are widely studied. Studies have proved that the methanol and water crude extracts of mushrooms have the effect of free radical scavenging and inhibiting the hemolysis of rat red blood cells. Oxygen anions also have a ce...

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Problems solved by technology

[0003] However, the current method for extracting active substances from mushrooms takes a long time to extract, generally a cycle takes a long time, and the extraction rate is not high, resulting in waste of raw materials, and cannot quickly and efficiently extract active substances from mushroom products, so further improvement is needed