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Method for joint production of ethanol and microbial lipid by using methyl cellulose as raw material

A microbial oil and lignocellulose technology, which is applied in the fields of biofuel, fuel, petroleum industry, etc., can solve the problems of difficult management, slow growth of woody oil plants, difficulty in large-scale development and utilization, etc., to reduce waste liquid discharge, The effect of low price and reduced cost of nitrogen source

Active Publication Date: 2011-08-17
TSINGHUA UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although marginal soils are used to plant woody oil plants to provide oil raw materials for biodiesel production, woody oil plants grow slowly, are difficult to manage, and are limited by geographical and climatic conditions, making it difficult to develop and utilize them on a large scale in a short period of time

Method used

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  • Method for joint production of ethanol and microbial lipid by using methyl cellulose as raw material
  • Method for joint production of ethanol and microbial lipid by using methyl cellulose as raw material

Examples

Experimental program
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Effect test

Embodiment 1

[0035] The lignocellulosic raw material used is bagasse, and the place of origin is Nanning, Guangxi. With reference to "Pulp and Paper Analysis and Detection" (Shi Shulan, Pulp and Paper Analysis and Detection, Beijing: China Light Industry Press, 2003.), the bagasse components are analyzed by relevant measurement methods, and the analysis results are: in this bagasse, The ash content is 1.38%, the hot water extract is 5.16%, the 1% NaOH extract is 34.20%, the benzene-alcohol extract is 3.17%, the cellulose content is 44.98%, and the cellulose content is 76.76%. The acid-insoluble lignin content is 18.45%, and the acid-soluble lignin content is 1.80%.

[0036] Bagasse and 1% sulfuric acid solution were mixed at a liquid-to-solid ratio of 10:1 and pretreated at 160°C for 0.5h. The pretreated cellulose solids were analyzed, and the contents of glucan, xylan and total lignin were 56.0%, 2.37% and 40.6%, respectively. The solid was added with 2g / L ammonium sulfate, 5g / L potassi...

Embodiment 2

[0038] The bagasse used is the same as in Example 1.

[0039] Bagasse and 1% sulfuric acid solution were mixed at a liquid-to-solid ratio of 10:1 and pretreated at 160°C for 0.5h. The liquid phase was collected, filtered briefly and then poured into a foam separation device. Air was introduced from the bottom at a ventilation rate of 10L gas / L liquid / h, and foam generation was observed. After half an hour, the aeration was stopped, and the non-foaming liquid (residual liquid) was collected and evaporated under reduced pressure. After being concentrated 10 times, the pH was adjusted to 2.0 with calcium hydroxide. After the solid matter was removed by filtration, 2% activated carbon based on the liquid weight was used for detoxification treatment at 80° C. for 30 minutes, and then filtered to obtain a clear liquid. After adjusting the pH to 6.0 with calcium hydroxide, filter again to obtain a sugar-containing hydrolyzate. Measured by high performance liquid chromatography wher...

Embodiment 3

[0041] After the waste liquid 1 in the embodiment 1 and the waste liquid 2 in the embodiment 2 were mixed, they were evaporated under reduced pressure, so that the xylose concentration therein reached 50 g / L. Ammonium sulfate, yeast powder, potassium dihydrogen phosphate and magnesium sulfate were added to concentrations of 0.1 g / L, 0.75 g / L, 0.4 g / L and 1.5 g / L, respectively. After stirring evenly, sterilize at 115°C for 15 minutes. Then oleaginous yeast (Rhodosporidium toruloides, Rhodosporidium toruloides AS 2.1389) was inoculated according to the inoculum amount of 10%, and cultured at 30° C. for 168 hours. The concentration of residual sugar in the fermentation broth was determined to be: xylose 10.1g / L, arabinose 5.25g / L, and the biomass of bacteria was 14.5g / L. The fermentation broth was centrifuged to obtain the bacteria, and the oil was extracted by the acid-heat method. The oil content in the bacteria was analyzed to be 51.2%, and the oil yield was 18.6g oil / 100g co...

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Abstract

The invention provides a method for joint production of biological ethanol and microbial lipid by using methyl cellulose as raw material and then realizing the joint production of ethanol and biodiesel by using the methyl cellulose as the raw material. The method comprises the following steps of: (1) ethanol fermentation is carried out for a cellulose solid obtained after the methyl cellulose is pretreated; (2) a product of hemicellulose hydrolyzation in the pretreatment process is used for microbial lipid fermentation; (3) waste liquor produced after ethanol in ethanol fermentation is recycled and waste liquor produced after microbial lipid is fermented are used for microbial lipid fermentation; and (4) yeast fermented by alcohol and thallus residue produced after lipid extraction are hydrolyzed and then are used as a nitrogen source to be used for ethanol fermentation and microbial fermentation process. The method provided by the invention can convert the methyl cellulose with low grade into two biomass fuels of bioethanol and biodiesel and simultaneously can reduce waste water emission in ethanol production.

Description

technical field [0001] The invention belongs to the field of biochemical industry, and relates to a method for coproducing ethanol and microbial oil by using lignocellulose as raw material, which can further convert microbial oil into biodiesel, thereby realizing the coproduction of ethanol and biodiesel by using lignocellulose as raw material. Background technique [0002] In recent years, due to the depletion of fossil energy and the carbon dioxide, nitrogen oxide, sulfur oxide and black smoke emitted after combustion, which have caused serious environmental pollution problems, the development of renewable alternative energy sources, such as biofuels, has received a lot of attention from all over the world. widespread attention. Among them, bioethanol and biodiesel are the two most concerned biofuels. [0003] Bioethanol can be produced from starchy raw materials such as corn and wheat through microbial fermentation, but the production of bioethanol from grains will cause...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C10L1/02C11C3/10C12P7/64C12P7/10
CPCY02E50/13Y02E50/16Y02E50/10
Inventor 赵雪冰刘德华杜伟徐静阳
Owner TSINGHUA UNIV
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