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Method for preparing low-fluorine Euphausia superba protein base materials

A technology of Antarctic krill and protein, which is applied in the direction of protein food ingredients, fish protein ingredients, food science, etc., can solve the problems of less research on defluorination, reduce the fluorine content of Antarctic krill, etc., and achieve wide application, simple and effective method Effect

Inactive Publication Date: 2010-04-07
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
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Problems solved by technology

However, at present, there are few researches on the defluorination of Antarctic krill before eating and the defluorination of Antarctic krill protein extraction process, and there are no domestic patents; the only foreign patent is UK2240786A, which uses an electrode enrichment method to reduce the fluorine content of Antarctic krill. The results showed that after 120 minutes of treatment with an aluminum electrode, the fluorine content of live krill reached 95ppm and 15ppm, respectively. However, the results Still higher than the requirements of GB2762-2005 food pollutant fluorine limit standard: fish (fresh water) should be less than 2.0mg / kg

Method used

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Embodiment

[0011] Antarctic krill was frozen at -22°C for 2 hours, crushed with a hammer mill; water was added to the crushed krill, the amount of water added was 6 liters per kilogram of crushed krill, stirred and mixed, homogenized, and adjusted to obtain The pH of the homogenate was 11.5 (adjusted with NaOH aqueous solution), stirring and leaching at 20° C. for 30 minutes, and centrifugal separation to obtain liquid phase A and precipitate A. Add water to the precipitate A, the amount of water added is 3.0 liters per kilogram of precipitate A, stir and mix, adjust the pH value to 11.5 (adjusted with NaOH aqueous solution), stir and extract at 20°C for 30 minutes, and centrifuge to obtain liquid phase B and Precipitation B. Combine liquid phase A and liquid phase B to obtain liquid phase C. The pH of the liquid phase C was adjusted to 4.6 (adjusted with aqueous HCl solution), the layers were separated, and the precipitate C was obtained. Add water to the precipitate C, the amount of ...

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PUM

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Abstract

The invention provides a method for preparing low-fluorine Euphausia superba protein base materials. The method is characterized by freezing and grinding the Euphausia superba, extracting proteins under alkali condition, precipitating and separating the proteins under acid condition and acid-washing the precipitated proteins for defluorination, thus preparing the products. The water contents of the products are 82-87% and the fluorine contents of the products are lower than 2mu g / g wet basis. The method is simple and effective, can ensure the recovery rate of the crude protein of the Euphausia superba to be 39-46%, the recovery rate of the crude lipid to be 38-42%, the content of the crude protein to be 66-71g / 100g wet basis, the content of the crude lipid to be 27-30g / 100g wet basis and the content of phospholipid to be 380-420mg / g lipid and ensure the products to have high nutritional values. The method is suitable for industrial application and the prepared products are wide in use and can serve as the accessories or base materials for preparing the Euphausia superba protein powder and other food.

Description

technical field [0001] The invention relates to a preparation method of low-fluorine Antarctic krill protein base material. Background technique [0002] Krill belongs to Crustacea and Euphausiacea in classification. There are as many as 11 species of krill in Antarctic waters, but the one with the largest number and the largest individual is called Antarctic krill, which is commonly called Antarctic krill (Euphausia superba). 6cm, translucent red, likes to live in groups, and belongs to cold-loving aquatic animals, so it is mainly distributed in the radiation belt not exceeding the Southern Ocean. [0003] Antarctic krill is one of the largest animal protein banks on Earth. The estimated biological reserves of Antarctic krill are 400-1.55 billion tons, and the annual sustainable catch is 0.7-200 million tons. At present, the total annual catch of fish and other aquatic products in the world is about 130 million tons. The water content of Antarctic krill is 77.9-83.1%, an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23J1/04
Inventor 薛长湖王灵昭王玉明李兆杰薛勇
Owner OCEAN UNIV OF CHINA
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