Lipid-rich chlorella and culture application thereof

A chlorella and oil technology, applied in the fields of biotechnology and bioenergy, can solve problems such as poor nitrogen fixation effect, achieve the effects of alleviating the problem of the greenhouse effect, solving the source of oil, and high fixation efficiency

Active Publication Date: 2019-04-05
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The invention can realize the CO emission in high-concentration complex environments such as waste gas...

Method used

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  • Lipid-rich chlorella and culture application thereof
  • Lipid-rich chlorella and culture application thereof
  • Lipid-rich chlorella and culture application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 Separation and domestication screening to obtain Chlorella SF-B1

[0024] (1) Obtaining the starting algae strain: In October 2011, 50mL of water samples were retrieved from Suifenhe, Heilongjiang, and inoculated into 200mL of BG11 medium for enrichment culture. The light-dark cycle is 24 hours, and the light-dark time ratio is 14:10. After culturing for about half a month, the culture medium turns green. Dilute the water sample of the enrichment culture to 10 -5 times, spread it on the BG11 solid plate under sterile conditions and cultivate it. The light intensity of the culture is 5000Lux and the temperature is 25°C. After about 10 days of cultivation, green single algae appear on the plate, and the single algae is picked and dropped on the shaker flask. Cultivation, the cultivation temperature is 25°C, and the light intensity is 8000 Lux. After 8 days of cultivation, observe under a microscope to determine whether it is a pure algae species. If it is not a...

Embodiment 2

[0029] Example 2 Identification of algae strains

[0030] The DNA of the obtained SF-B1 algae cells was extracted using the CTAB method, and the 18S rDNA gene was cloned, and the 3 positive clones obtained were sent to Shanghai Sangong Company for sequencing. The results of 18S rDNA gene sequencing analysis are shown in the sequence table. The 18S rDNA sequence was logged into the Genbank database for Blast comparison, and the results showed that it was consistent with Chlorella sp. There is the greatest similarity, its BLASTn value is 2619, Max index value is 99%, it can be determined that SF-B1 is Chlorella ( Chlorella sp. ).

Embodiment 3

[0031] Example 3 The cultivation and application of Chlorella SF-B1

[0032] The algae liquid of SF-B1 in the logarithmic growth phase was inoculated in BG11 medium for cultivation. The formula of BG11 medium is shown in Table 1 and Table 2. The cultivation was carried out in a photobioreactor. The OD of the culture medium after inoculation was 690 is 0.2. CO was introduced from the bottom of the reactor 2 Flue gas with a content of 40v%, of which the NO content is 700×10 -6 (v / v). During the cultivation process, the light intensity was 8000Lux, the cultivation temperature was 28°C, the pH value was controlled at 7-8, the light cycle was 24h, the light-dark time ratio was 14:10, and the cultivation time was 7 days in a stable period. At the end of the cultivation, the algae fluid was collected by centrifugation, vacuum freeze-dried to constant weight at -60°C, and then the dry weight of the algae powder was measured to calculate the biomass production, and the total lipid c...

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Abstract

The invention relates to lipid-rich chlorella and culture application thereof. The strain is SF-B1, is classified and named as Chlorella sp., and has been preserved in the General Microbiological Center of the China Microbiological Culture Collection Management Committee on July 6, 2015, and the preservation number is CGMCC No. 11005. The chlorella can tolerate high-concentration CO2 and NOx, thecarbon fixation efficiency is high, and the total lipid content of cells in obtained biomass is 45% or above of the dry weight of the cells.

Description

technical field [0001] The invention belongs to the fields of biotechnology and bioenergy, and in particular relates to a strain of chlorella rich in oil and its cultivation application. Background technique [0002] Among many biomass energy sources, microalgae is an important renewable resource. They have the characteristics of wide distribution, large biomass, high photosynthetic efficiency, strong environmental adaptability, short growth cycle, and high biomass yield. Its cells contain unique primary or secondary metabolites with complex chemical composition. The solar energy conversion efficiency of microalgae can reach 3.5%, which is a potential resource for the production of pharmaceuticals, fine chemicals and new fuels. The fatty acids obtained from microalgae can be converted into fatty acid methyl ester, that is, biodiesel. Therefore, biodiesel produced using microalgae oil as a raw material is currently the most likely renewable energy source to meet the world's...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12P7/64B01D53/84B01D53/56B01D53/62C12R1/89
CPCB01D53/56B01D53/62B01D53/84C12N1/12C12P7/6463B01D2258/0283C12R2001/89C12N1/125Y02P20/59Y02A50/20
Inventor 师文静王鹏翔李晓姝孙启梅张霖樊亚超
Owner CHINA PETROLEUM & CHEM CORP
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