Freshwater Chlorella sorokiniana GS30 and application thereof
A technology of chlorella, CCTCCM2013607, applied in the field of biomass energy, can solve the problem of high production cost of microalgae biodiesel, achieve high-concentration carbon dioxide fixation efficiency, biomass yield and oil yield, high-efficiency utilization, and reduce production cost effect
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Embodiment 1
[0050] Under sterile conditions, single algae were picked from a solid plate and dropped into a 100ml Erlenmeyer flask containing 30ml sterilized BG-11 culture solution, and cultured statically in a light culture rack at a temperature of 28°C and a light intensity of 7500lux for 12 days. Grow to the end of the index and expand the culture with an inoculation ratio of 1:10.
[0051] Prepare 2.4L of BG-11 medium, insert the expanded cultured cells, and inoculate the cells with optical density OD 680 =1.2. The initial pH is 4.0, the temperature is 15°C, the light-to-dark ratio is 8:16, and under the light intensity of 15000lux, 0.5vvm of air (CO 2 The content is 0.03% (v / v)), cultivated for 14 days.
[0052] The algal cells were collected by centrifugation, dried in a vacuum freeze dryer, and the algal powder was weighed and the dry weight was calculated. The biomass concentration of algal powder is 680mg / L, the biomass production rate is 70mg / L / d, CO 2 The fixation efficienc...
Embodiment 2
[0054] Pick a single algae on a solid plate under sterile conditions and drop it into a 100ml Erlenmeyer flask containing 30ml of sterilized culture solution, and place it in a light culture rack for static culture. The temperature is 28°C, and the light intensity is 7500lux. Cultivate for 12 days and grow to an index. At the end of the period, the culture was expanded with a 5% inoculation ratio.
[0055] Prepare 3.1L of BG-11 medium and insert it into the expanded cultured cells. The initial pH is 7.0, the temperature is 45°C, the light-to-dark ratio is 24:0, under the light intensity of 2500lux, 1vvm of 30% CO is introduced 2 (v / v), cultured for 10 days.
[0056] The algal cells were collected by centrifugation, dried in a vacuum freeze dryer, and the dry weight of the algal powder was weighed and calculated. The biomass concentration of algae powder is 2400mg / L, the biomass production rate is 360mg / L / d, CO 2 The fixation efficiency is 660mg / L / d, the oil content determin...
Embodiment 3
[0058] Pick a single algae on a solid plate under sterile conditions and drop it into a 100ml Erlenmeyer flask containing 30ml of sterilized culture solution, and place it in a light culture rack for static culture. The temperature is 28°C, and the light intensity is 7500lux. Cultivate for 12 days and grow to an index. In the final stage, the inoculation ratio was 1:10 to expand the culture.
[0059] Prepare 3.1L of BG-11 medium and insert it into the expanded cultured cells. The initial pH is 11.0, the temperature is 10°C, the light-to-dark ratio is 12:12, and 0.2vvm of 45% CO is introduced under the light intensity of 9500lux. 2 (v / v), cultured for 14 days.
[0060] The algal cells were collected by centrifugation, dried in a vacuum freeze dryer, and the dry weight of the algal powder was weighed and calculated. The biomass concentration of algae powder is 2500mg / L, the biomass production rate is 260mg / L / d, CO 2 The fixation efficiency is 480mg / L / d, the oil content determ...
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