Preparation method and application of denitrifying bacteria immobilized on graphene-based composite material
A technology of denitrifying bacteria and composite materials, which is applied in the field of preparation of immobilized denitrifying bacteria on graphene-based composite materials, can solve problems such as instability, limited use, and low mechanical strength, and achieve enhanced resistance and increased tolerance , good effect
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Embodiment 1
[0036] A preparation method for graphene-based composite immobilized denitrifying bacteria, the process steps comprising:
[0037] 1) Take two rings of paracocci from the inclined surface and inoculate them into the enrichment medium, culture on a shaking table, centrifuge the culture medium to remove the supernatant, and obtain bacterial cells; suspend the bacterial cells in sterile normal saline, and obtain denitrifying bacteria suspensions. liquid; the concentration of sterile saline is 0.9wt%; the mass ratio of bacteria to sterile saline is 1:30. When the culture medium is cultured on a shaker, the rotation speed is 150r / min, the culture temperature is 27°C, and the culture time is 40h.
[0038] 2) Add 1wt% charcoal powder to the bacterial suspension to absorb for 4 hours to obtain an adsorption solution;
[0039] 3) Prepare graphene-based composite materials by covalent cross-linking method, the specific operation is as follows: add polyvinyl alcohol and sodium alginate ...
Embodiment 2
[0043] A preparation method for graphene-based composite immobilized denitrifying bacteria, the process steps comprising:
[0044] 1) Take two rings of paracocci from the inclined surface and inoculate them into the enrichment medium, culture on a shaking table, centrifuge the culture medium to remove the supernatant, and obtain bacterial cells; suspend the bacterial cells in sterile normal saline, and obtain denitrifying bacteria suspensions. liquid; the concentration of sterile saline is 0.9wt%; the mass ratio of bacteria to sterile saline is 1:10. When the culture medium is cultured on a shaker, the rotation speed is 150r / min, the culture temperature is 30°C, and the culture time is 48h.
[0045] 2) Add 1wt% charcoal powder to the bacterial suspension for adsorption for 6 hours to obtain an adsorption solution;
[0046] 3) Prepare graphene-based composite materials by covalent cross-linking method, the specific operation is as follows: add polyvinyl alcohol and sodium algina...
Embodiment 3
[0050] Application of graphene-based composite material immobilized denitrifying bacteria prepared by the present invention in degrading nitrate nitrogen sewage:
[0051] 1. adopt the method as described in embodiment 1 to prepare graphene-based composite immobilized denitrifying bacteria;
[0052] 2. adjust the artificial sewage nitrate nitrogen content to be 500mg / L, as shown in Table 1, the chemical oxygen demand (abbreviated as COD) pollutant content is 800mg / L, add the prepared graphene-based composite immobilized denitrifying bacteria, add The wet weight is 15g / L, the pH of the solution is 8, and then in a shaker at 180r / min at 30°C until the nitrate nitrogen is fully degraded.
[0053] Table 1 Simulated artificial sewage composition
[0054] Element
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