Industrialized production method of pectase

Abstract

The invention discloses an industrialized production method of pectase with high enzyme activity, which comprises the following steps: bacterial strain selecting and breeding, strain culturing, flask-shaking and fermenting as well as enzyme solution preparation. The industrialized production method is characterized in that the pectase, namely, bacillus cereus is obtained by the separation and thescreening of natural-collecting bacterial samples and strain mutagenesis under the cellular level, the laboratory number is MAPLE21, the conversation number is CCTCC NO: M209147, and the pectase has the following biological properties: bacilliform thallus, even dyeing, G<+>, facultative aerobe, spores forming without exposing out of the thallus, leveler two ends of the thallus and chain-like arrangement of a majority of spores. The invention has the advantages of high enzyme activity, low cost, short fermentation period, and the like; when the invention is used for bast fiber degumming, the degumming time (2-4h) is short and stable, the defective gum ratio is below 1.5 percent, the destruction to fiber strength is small, the fiber dispersion ratio is 100 percent, the finished product ratio is above 65 percent, and the generation amount of degumming waste water is reduced by more than 80 percent.

Description

Technical field: [0001] The invention relates to an enzyme and its production, in particular to an industrial production method of pectinase. Background technique: [0002] At present, the degumming process of bast fibers such as ramie, hemp, flax, mulberry bark and mulberry bark is basically a chemical degumming process based on caustic soda. Although the plant fiber products produced by this production process can meet the requirements of carding and spinning, there are still There are disadvantages such as long degumming process, cumbersome process, high labor intensity, high energy consumption, and large environmental pollution. At the same time, due to the use of strong acid, strong alkali, high temperature and high pressure scouring and other caustic conditions, some cellulose fiber molecular chains are broken and crystallinity is reduced. Increased height causes problems such as low product yield, strong rigidity, and rough fabric feel. Pectin in plant cells is mainl...

AI Technical Summary

Problems solved by technology

Although a large number of experts and scholars have carried out in-depth research on bast fiber adopting biological enzyme degumming in recent years, as the patent No. is ZL01106844. The patent of its application in the ramie degumming process discloses that a kind of enzyme liquid prepared by Bacillus subtilis (Bacillus subtilis), the laboratory number is No.13, and the bacterial classification of preservation number CCTCCNO: M200038 is applied to the ramie degumming process, but There are problems such as low enzyme activity (3000u / ml-4000u / ml), fermentation period of more than 30 hours, high enzyme production cost, and difficulty in industrialized production and popularization and application.