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Method for degumming by using immobilized phospholipase C

A technology of immobilized phospholipase and immobilized enzyme, which is applied in the direction of fat production, fat oil/fat refining, etc., and can solve problems such as high cost, difficult separation and recovery, and difficult enzyme production

Inactive Publication Date: 2012-12-05
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The present invention is aimed at the miscibility of free enzymes in the reaction system, which is not only difficult to separate and recover, but also generally difficult to prepare enzymes, and the cost is high, so it is difficult to use repeatedly or continuously, and the phospholipids in oils and fats are mainly divided into hydratable phospholipids and non-hydratable phospholipids. Phospholipids and hydrated phospholipids can be removed by traditional hydration degumming methods, but non-hydratable phospholipids have significant hydrophobicity and are difficult to remove, and the proposed method of degumming with immobilized phospholipase C

Method used

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specific Embodiment approach 1

[0006] Embodiment 1: A method for degumming immobilized phospholipase C is realized through the following steps: 1. Immobilization of phospholipase C: first weigh a certain amount of sodium alginate and dissolve it in a water bath at 40°C, and weigh a certain amount at the same time Dissolve chitosan in 5% acetic acid solution in a 40°C water bath, then add a certain amount of CaCl2 solution to the completely dissolved chitosan acetic acid solution, and mix well. Weigh a certain amount of phospholipase C, add it to 10 mL sodium alginate solution, stir evenly, let it stand for a while, draw the mixed solution with a sterilized syringe, and inject chitosan acetate at a speed of about 5 drops / s solution and CaCl2 mixed solution, stirred at 180 r / min, placed at 4°C for 10 h; 2. Degumming of immobilized enzyme: Take 300 g of hydrated soybean oil, preheat to 75°C, add 45% 0.6 mL of citric acid was mixed and stirred for 30 min. Cool to a temperature of about 30~40°C, add 4mol / L NaOH...

specific Embodiment approach 2

[0007] Specific embodiment 2: The difference between this embodiment and specific embodiment 1 is that in step 2, the initial pH value in the oil phase is 5.5-8.0 to immobilize phospholipase C to degumming soybean oil. Other steps and specific implementation Method 1 is the same.

specific Embodiment approach 3

[0008] Specific embodiment three: the difference between this embodiment and specific embodiment one is that in step two, the initial pH value in the oil phase is 5.5 to 8.0, and the reaction time is 30 to 90 minutes. Soybean oil degummed. Other steps are the same as in the first embodiment.

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Abstract

The invention provides a method for degumming soybean crude oil by using immobilized phospholipase C (PLC). According to the method, free PLC can hydrolyze phosphorus-oxygen bonds between phosphate groups and glyceryl specifically to generate diglyceride, and the diglyceride is dissolved in oil to form one part of edible oil, so that the yield of degummed oil can be improved; but a miscible natural enzyme is not easy to separate and recycle in a reaction system and is high in cost, so the miscible natural enzyme cannot be used repeatedly or continuously. The immobilized PLC is added into the soybean crude oil to perform degumming, and the phosphorus content and the yield of grease of the degummed soybean crude oil and the repeated using time of the immobilized PLC are determined, so that after the soybean crude oil is degummed, the minimum phosphorus content is 3.8 mg / kg, the yield of the grease is improved by 1.2 times, and after the immobilized PLC is used for 6 batches continuously, the relative vigor of the immobilized PLC is still kept about 80 percent. Therefore, degumming is performed by using the immobilized PLC, so that the refining rate can be improved, and the production cost can also be reduced.

Description

technical field [0001] The invention relates to a method for degumming immobilized phospholipase C. Background technique [0002] In the oil processing industry, oil degumming has always been one of the focuses of people's attention. Degumming is the removal of peptized impurities in oil, and the main component is phospholipid. Phospholipids are mainly divided into hydratable phospholipids and non-hydratable phospholipids. Hydrated phospholipids can be removed by traditional hydration degumming methods, but non-hydratable phospholipids have significant hydrophobicity and are difficult to remove. The traditional degumming methods mainly include hydration degumming, acid refining degumming, adsorption degumming, electropolymerization degumming, thermal coagulation degumming, super degumming, special wet degumming, ultrafiltration degumming and enzymatic degumming to treat crude oil and reduce gross Method for peptizing impurities in oils. Generally speaking, each method has ...

Claims

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Application Information

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IPC IPC(8): C11B3/00
Inventor 于殿宇江连洲张立国胡立志刘晶张春艳宋云花王玥宋鹏李万振刘鑫邹小雨
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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