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Method for degumming soybean crude oil by virtue of combined application of immobilized phospholipase A1 and immobilized phospholipase C

A technology of immobilized phospholipase and combined application, applied in the direction of fat oil/fat refining, fat production, etc., can solve the problems of difficult separation and recovery, difficult to repeat, high cost, etc.

Inactive Publication Date: 2015-04-29
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention conventionally adopts phospholipase A for enzymatic degumming 1 , the degummed product lysophospholipid has good hydrophilicity and is easy to wash and remove, but it takes too long, which brings difficulties to continuous operation. Phosphorus-oxygen bond between them to generate diacylglycerol, which is dissolved in oil and becomes a part of edible oil; at the same time, not only is it difficult to separate and recover free enzymes when they are miscible in the reaction system, but also the preparation of free enzymes is generally difficult. And the cost is high, it is difficult to use repeatedly or continuously, etc., it is proposed to use phospholipase A 1 , The respective characteristics of phospholipase C, developed an immobilized phospholipase C-phospholipase A 1 Double-enzyme degumming technology, that is, first using a small amount of immobilized phospholipase A 1 Carry out conventional enzymatic degumming operations, use lysophospholipids to form an emulsification system that is conducive to the subsequent immobilized phospholipase C, and then add an appropriate amount of immobilized phospholipase C to perform degumming operations

Method used

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specific Embodiment approach 1

[0006] Specific embodiment one: a kind of immobilized phospholipase A 1 Combined with immobilized phospholipase C, the method for degumming soybean crude oil is characterized in that immobilized phospholipase A 1 The method for degumming soybean crude oil with immobilized phospholipase C is realized through the following steps: 1. Immobilization of phospholipase A and phospholipase C: first weigh a certain amount of sodium alginate and dissolve it in a water bath at 40° C. A certain amount of chitosan was dissolved in 5% acetic acid solution in a 40°C water bath, and then a certain amount of CaCl was added to the dissolved chitosan acetic acid solution. 2 solution, mix well, weigh a certain amount of phospholipase A 1 , added to 10 mL sodium alginate solution, stirred evenly, let stand for a period of time, draw the mixed solution with a sterilized syringe, inject chitosan acetate solution, CaCl solution at a speed of about 5 drops / s 2 In the mixed solution, stir at 180 r / mi...

specific Embodiment approach 2

[0007] Specific embodiment two: The difference between this embodiment and specific embodiment one is that the degumming conditions of the immobilized enzyme in step two are further determined, and the result is phospholipase A 1 The initial pH value of the reaction system after the reaction is 6.5-8.0, the reaction time is 40-80min, the reaction temperature is 40-60°C, and the amount of immobilized enzyme added is 0.02%-0.06%. Degumming of crude soybean oil, immobilized phospholipase A 1 and immobilized phospholipase C were continuously used for the degumming of crude soybean oil. After using 6 batches, the relative activity of the immobilized enzyme was measured, and the result was that the enzyme activity remained at about 80%. At the same time, the oil after degumming the immobilized enzyme was obtained The rate was compared with the conventional enzymatic degumming or the oil yield after the two enzymes were degummed separately, and the immobilized phospholipase A 1 ...

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Abstract

The invention provides a method for degumming soybean crude oil by virtue of combined application of immobilized phospholipase A1 and immobilized phospholipase C. The method comprises the following steps: developing an immobilized phospholipase A1-immobilized phospholipase C two-enzyme degumming technology according to respective characateristics of the phospholipase A1 and phospholipase C, namely, performing conventional enzymic method degumming by adopting a small amount of immobilized phospholipase A1, forming an emulsifying system favorable for the subsequent immobilized phospholipase C effects by utilizing lysophosphatide, and adding a proper amount of the immobilized phospholipase C so as to realize degumming. The lowest obtained degummed phosphorus content is 2.8mg / kg, and after the phospholipase C is continuously used in six batches, the relative activity of the immobilized enzyme is still maintained to be about 80 percent. Therefore, a combined degumming method is adopted, so that the refining rate can be improved, the production cost can be reduced, the degumming reaction time can be reduced by 2 hours, and the oil yield is improved by 1.3 times.

Description

technical field [0001] The invention relates to an immobilized phospholipase A 1 Combined application with immobilized phospholipase C to degumming soybean crude oil. Background technique [0002] Vegetable oil refining generally includes processes such as degumming, neutralization, decolorization and deodorization. The two main processes are divided into chemical refining and physical refining. Degumming is the first process of crude oil refining, which is used to remove phospholipids and mucilage substances from crude oil. Enzyme method has its unique advantages, such as specific reaction, mild reaction conditions, and less environmental pollution, making it more competitive in the development of new products with higher added value or unique nutritional and functional aspects force. Compared with the traditional water degumming process, enzymatic degumming reduces the use of a large amount of acid and alkali, reduces the waste water generated during the refining proce...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11B3/00
CPCC11B3/003
Inventor 于殿宇江连洲解桂东王立琦刘飞孙丽雪梁宝生李俊
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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