Method for degumming soybean crude oil by virtue of combined application of immobilized phospholipase A1 and immobilized phospholipase C

Abstract

The invention provides a method for degumming soybean crude oil by virtue of combined application of immobilized phospholipase A1 and immobilized phospholipase C. The method comprises the following steps: developing an immobilized phospholipase A1-immobilized phospholipase C two-enzyme degumming technology according to respective characateristics of the phospholipase A1 and phospholipase C, namely, performing conventional enzymic method degumming by adopting a small amount of immobilized phospholipase A1, forming an emulsifying system favorable for the subsequent immobilized phospholipase C effects by utilizing lysophosphatide, and adding a proper amount of the immobilized phospholipase C so as to realize degumming. The lowest obtained degummed phosphorus content is 2.8mg/kg, and after the phospholipase C is continuously used in six batches, the relative activity of the immobilized enzyme is still maintained to be about 80 percent. Therefore, a combined degumming method is adopted, so that the refining rate can be improved, the production cost can be reduced, the degumming reaction time can be reduced by 2 hours, and the oil yield is improved by 1.3 times.

Description

technical field [0001] The invention relates to an immobilized phospholipase A 1 Combined application with immobilized phospholipase C to degumming soybean crude oil. Background technique [0002] Vegetable oil refining generally includes processes such as degumming, neutralization, decolorization and deodorization. The two main processes are divided into chemical refining and physical refining. Degumming is the first process of crude oil refining, which is used to remove phospholipids and mucilage substances from crude oil. Enzyme method has its unique advantages, such as specific reaction, mild reaction conditions, and less environmental pollution, making it more competitive in the development of new products with higher added value or unique nutritional and functional aspects force. Compared with the traditional water degumming process, enzymatic degumming reduces the use of a large amount of acid and alkali, reduces the waste water generated during the refining proce...

AI Technical Summary

Problems solved by technology

[0004] The present invention conventionally adopts phospholipase A for enzymatic degumming 1 , the degummed product lysophospholipid has good hydrophilicity and is easy to wash and remove, but it takes too long, which brings difficulties to continuous operation. Phosphorus-oxygen bond between them to generate diacylglycerol, which is dissolved in oil and becomes a part of edible oil; at the same time, not only is it difficult to separate and recover free enzymes when they are miscible in the reaction system, but also the preparation of free enzymes is generally difficult. And the cost is high, it is difficult to use repeatedly or continuously, etc., it is proposed to use phospholipase A 1 , The respective characteristics of phospholipase C, developed an immobilized phospholipase C-phospholipase A 1 Double-enzyme degumming technology, that is, first using a small amount of immobilized phospholipase A 1 Carry out conventional enzymatic degumming operations, use lysophospholipids to form an emulsification system that is conducive to the subsequent immobilized phospholipase C, and then add an appropriate amount of immobilized phospholipase C to perform degumming operations