58 results about "Endothelial proliferation" patented technology

The invention provides methods of attenuating, e.g., inhibiting or reducing, cellular proliferation and migration, particularly endothelial cell proliferation and migration, including that associated with angiogenesis, using opioid antagonists, including, but not limited to, those that are peripherally restricted antagonists.

The present invention concerns an antibody or fragment thereof, capable of binding to CD 160, said antibody comprising a) a light chain comprising three light chain complementary regions (CDRs) having the following amino acid sequences: (i) the light chain CDR1: QSISNH (SEQ ID NO: 1), (ii) the light chain CDR2: YAS, (iii) the light chain CDR3: QQSNSWPLT (SEQ ID NO: 2), and a light chain framework sequence from an immunoglobulin light chain; and b) a heavy chain comprising three heavy chain complementary regions (CDRs) having the following amino acid sequences: (i) the heavy chain CDR1: GYTFTDYW (SEQ ID NO: 3), (ii) the heavy chain CDR2: IYPGDDDA (SEQ ID NO: 4); (iii) the heavy chain CDR3: ARRGIAAVVGGFDY (SEQ ID NO: 5); and a heavy chain framework sequence from an immunoglobulin heavy chain; a pharmaceutical comprising said antibody and the use of said antibody for preparation of a medicament for treating and/or preventing a pathology associated with endothelial cells proliferation engaged in an angiogenesis process.

The invention belongs to gene engineering field. The invention establish a production method of human skeletal muscle troponin I for quick contract: recombing DNA to construct expression plasmid, refining expression products with inclusion bodies, cation exchange resin column chromatography, cation exchange resin column chromatography, renaturing inclusion bodies, at last purifying and obtaining recombinant human troponin I for suppressing blood vessel endothelial cell propagation and angiogenesis. The invention also establishes a method for determing the biological activity index of troponin I by suppressing Acto-S1 Mg-ATPase activity. The troponin I can prepare drugs of the tumor and angiogenesis related disease

The invention relates to application of Metrnl protein or gene in treatment of endothelial function impairment. On one hand, based on a Metrnl endothelial specific knockout mouse model, it is proved that due to lacking of Metrnl in blood vessel endothelia, an endothelium-dependent relaxation function is lowered, while Metrnl has the function of dilating blood vessels; on the other hand, based on a human umbilical vein endothelial cell line and Metrnl endothelial specific knockout mouse primary endothelial cells, it is proved that the cell proliferation is lowered after knockout of blood vessel endothelia Metrnl, showing that Metrnl has the effect of promoting endothelial cell proliferation. Therefore, the Metrnl protein or gene and a synergist of the Metrnl protein or gene can be used for preparing reagents for treating endothelium-dependent blood vessel relaxation dysfunction or regulating endothelial cell proliferation, and are applied to development of drugs of diseases related to endothelial function impariment.

Provided herein is a method for inducing formation of a conformationally distinct superfibronectin by contacting plasma fibronectin with BBK32 protein or a BBK32 peptide and the BBK32-induced superfibronectin so formed. Also provided are methods of inhibiting endothelial cell proliferation, of treating pathophysiological conditions associated with endothelial cell proliferation and of inhibiting angiogenesis therein using the BBK32 protein, BBK32 peptide or the BBK32-induced superfibronectin.

Murine and humanized anti-human VEGF antibodies and uses are disclosed. The anti-human VEGF antibodies of the invention have higher binding affinity for human VEGF-A, are stronger inhibitors of the VEGF-A induced proliferation of endothelial cells in culture as compared with anti-human VEGF antibodies in the art. Moreover, these antibodies cross react with human VEGF-B. The antibodies of the invention inhibit tumor growth in vivo in greater extent than Bevacizumab (Avastin™) when administered at the same dosage

The invention relates to a traditional Chinese medicine patch for the prevention and early-stage curing of bronchial asthma, and the treatment of a plurality of respiratory diseases and megrims, characterized in that the traditional Chinese medicine patch comprises, based on the weight percentage, 57 to 70 % of peach seed, 12 to 25 % of cape jasmine, 6 to 8 % of almond, and 5 to 10% of pepper. The invention has the advantages that the traditional Chinese medicine patch is mainly used for treating bronchial asthma, can cure the bronchial asthma of children at the age of below 12, and can be used in the outbreaking or catabatic period. The traditional Chinese medicine patch has the efficacy of diminishing inflammation, invigorating blood circulation, removing blood stasis, clearing away cold, relieving cough and calming panting. The traditional Chinese medicine patch can promote the synthesis of the alveolar surfactant, protect the pulmonary function to improve the lesion tissues, and can stimulate the proliferation of blood vessel endothelial cells to restore the endangium. The traditional Chinese medicine patch has the antiallergic effect, can inhibit the antiserum reaction of allergic antibody of the skin, reduce the pigment seepage and alleviate the airway obstruction. The traditional Chinese medicine patch can enhance the proliferation and conversion of T lymphocyte of spleen of a body, and improve the autoimmunity.

The invention discloses a substituted 2,3-dihydro-4(1H)- quinazolone derivative shown in the formula I, pharmaceutically acceptable salt or solvate and a drug composite containing the same and application thereof. The compound has very good effect of restraining vascular endothelial cell proliferation. The compound, the pharmaceutically acceptable salt or solvate or the drug composite containing the same can be used for drugs requiring to restraining angiogenesis diseases and/or cytotoxicity exerting diseases.

The invention discloses a molecular marker for early warning of atherosclerosis. A method comprises the following steps that (1) the influence of Hcy (Homocysteine) with different concentrations on endothelial cell proliferation is detected by an MTT colorimetric method, and a proper concentration is screened out; (2) after cells is intervened with the Hcy with optimal concentration, cell viability staining detection is carried out; (3) after the cells is intervened with the Hcy with optimal concentration, the apoptosis of endothelial cells is detected by flow cytometry; (4) after the cells isintervened with the Hcy with optimal concentration, the expression of LncRNA (long noncoding RNA) DINO (Damage Induced Noncoding) is detected through fluorescent quantitative PCR in real time; (5) transfecting of the LncRNA DINO siRNA is carried out, after the LncRNA DINO is interfered, and then cell viability staining detection is carried out; (6) LncRNA DINO siRNA is constructed, after the LncRNA DINO is interfered, and the apoptosis of endothelial cells is detected by flow cytometry; and (7) LncRNA DINO siRNA is constructed, after the LncRNA DINO is interfered, and then the expression of apoptosis-related protein is detected by protein immunoblotting. A novel molecular target for early diagnosis and early warning of atherosclerosis is provided, and a new direction is provided for treatment.

The invention relates to a heparin-loaded covered stent for treating arterial aneurysm and a preparation method thereof. The covered stent is characterized in that a covered layer is formed by heparin-loaded emulsion electrostatic spinning nanofibers, wherein the heparin-loaded nanofibers are provided with multi-core-layer structures. The preparation method of the covered stent comprises the following steps of arranging a preparation device, compounding a heparin sodium aqueous solution, preparing heparin sodium aqueous solution-sorbide oleate-dichloromethane emulsion, preparing an electrostatic spinning solution, and preparing a loading heparin covered stent. By adopting the covered stent, the problems which are common in treatment of small vessel diseases such as vascular endothelial proliferation and thrombosis can be effectively avoided; the heparin-loaded covered stent has a wide application prospect in the field of intravascular interventional treatment of small vessels arterial aneurysm; and the preparation method has the advantages that the preparation device is simple, procedure parameters are less, and the preparation process is stable.

Compositions and methods effective in inhibiting abnormal or undesirable cell proliferation, particularly endothelial cell proliferation and angiogenesis related to neovascularization and tumor growth are provided. The compositions comprise a naturally occurring or synthetic protein, peptide, or protein fragment containing all or an active portion of the C-terminal portion of proteinase inhibitors such as TFPI. The methods involve administering to a human or animal the composition described herein in a dosage sufficient to inhibit cell proliferation, particularly endothelial cell proliferation. The methods are useful for treating diseases and processes mediated by undesired and uncontrolled cell proliferation, such as cancer, particularly by inhibiting angiogenesis. Administration of the composition to a human or animal having prevascularized, metastasized tumors is useful for preventing the growth or expansion of such tumors.

The invention provides a multifunctional aerogel dressing for promoting wound healing and a preparation method thereof. The preparation method of the multifunctional aerogel dressing comprises the following steps that S1, an amino acid derivative, an antibacterial polypeptide derivative and natural polysaccharide are dispersed in deionized water, and a clear solution is obtained after heating; S2,the clear solution containing the amino acid derivative, the antibacterial polypeptide derivative and the natural polysaccharide is guided into into a mold, and cooling is performed to room temperature to form hydrogel; and S3, the hydrogel is subjected to freeze drying, and the multifunctional aerogel dressing is prepared. The invention provides the multifunctional wound dressing material capable of promoting proliferation of skin fibroblasts and endothelial cells, resisting bacteria in a broad spectrum and absorbing exudate and the preparation method of the multifunctional wound dressing material. The problems that an existing wound dressing is single in function and cannot effectively promote wound healing and prevent wound infection are solved.

The present invention relates to a method for expression and purification of human troponin C(TnC) and its application using said protein as medicine for resisting angiogenesis. It adopts PCR method,and includes the following steps: obtaining coded sequence of human troponin C from human mammary gland cDNA library; constructing colibacillus expression plasmid containing said coded sequence, pET36-TnC and its transformed colibacillus engineering bacterium strain E. coli BL21(DE3)pLyS/pET36-TnC; and utilizing said engineering bacterium expression to produce human troponin C with biological activity white can inhibit vascular endothelial proliferation and inhibit tumor growth. It can be used as medicine for curing tumor.

Stable water soluble polypeptides which are potent inhibitors of endothelial cell proliferation and of angiogenesis. Polypeptide inhibitors of endothelial cell growth may have important uses in the elucidation of the mechanism of angiogenesis, disease diagnosis and prognostication, and drug therapies for use in animals and humans.

The invention discloses a cortex albiziae lignan glycoside compound promoting endothelial cell proliferation and application, and belongs to the technical field of biomedicine. New application of Icarisid E5 in promoting the proliferation and migration of endothelial cells and promoting angiogenesis is provided. It is confirmed by experiments that the HUVEC cell viability after administration of 40 [mu]M for 24 h can be increased to 133.65% of the control, cell migration can be significantly promoted, and the function of promoting wound healing in in-vivo experiments is achieved. The preparedmonomeric compound Icarisid E5 can significantly promote cell proliferation activity, promote the migration and self-replication of HUVEC cells, can be used for transferring exogenous angiogenesis-inducing factors into tissue, promotes angiogenesis, and enhances cell reproductive capacity.

The invention relates to the medicine field, in particular to polypeptide capable of promoting profibrinolysin to be hydrolyzed into angiostain and treating a malignant tumor. The sequence of the polypeptide is THELGHLSQKLYGKRSN and is a brand new sequence, the polypeptide can promote the hydrolyzation of the profibrinolysin in vitro and inhibit the proliferation of vascular endothelial cells, in a vivo test, the survival rate of tumor-bearing mice is improved, and the polypeptide has a potential new drug development value.

The invention provides a method for verifying the Eltrombopag (ELB) mediated anti-angiogenesis function of macrophage. Through MTT assay, q-PCR and ELISA (enzyme linked immunosorbent assay), influenceof the ELB upon macrophage proliferation, expression of mRNA (messenger ribonucleic acid) of related angiogenesis factors (VEGF-A, bFGF, MMP-9) and VEGF (vascular endothelial growth factor) proteinscan be detected; and furthermore, through MTT experiments, cell wound scratch assay and Matrigel pipe formation experiments, influence of the ELB upon endothelial cell proliferation, migration and tube cavity formation through macrophage can be observed. The method verifies that the ELB is capable of inhibiting proliferation of the macrophage, inhibiting mRNA of the expression of related angiogenesis factors and inhibiting release of the VEGF proteins, and meanwhile, finds that the ELB acts on supernate of the macrophage and is capable of remarkably inhibiting proliferation, migration and tubule formation of the endothelial cells, and thus the macrophage can be mediated by the ELB and thus has the anti-angiogenesis function.