Preparation method for extracting high-quality protein peptide from pig lung

A protein peptide and high-quality technology, applied in the biological field, can solve problems such as waste of biological resources, food safety issues, and waste of discarding, and achieve the effects of short production cycle, low cost, and high nutritional value

Inactive Publication Date: 2018-10-09
陈石良
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to various reasons, except for a small amount of pig lungs that are directly eaten and partially processed into feed and fertilizer, most of them are discarded

Method used

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  • Preparation method for extracting high-quality protein peptide from pig lung

Examples

Experimental program
Comparison scheme
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Example Embodiment

[0019] Example 1

[0020] (1) Beating: Take 100 kilograms of fresh pig lungs, clean and decontaminate, strip the trachea, and remove grease, then use a chopper to mince it into a minced meat shape, and then grind and homogenize with a fine grinder for 5 minutes to obtain a pig lung slurry ;

[0021] (2) Degreasing: Add 300 kilograms of water to the lung slurry, stir and mix, adjust its pH to 8.5, heat to 43-45°C, add 120 grams of lipase, keep it warm for 60 minutes and pass 100 Mesh filtering, collecting filter residue;

[0022] (3) Enzymatic hydrolysis: add water twice its weight to the collected filter residue, stir evenly, adjust the pH to 8.8, add 2.5 grams of alkaline protease per kilogram of filter residue, heat to 42-45°C, keep the enzyme hydrolyzed for 9 hours , The enzymolysis solution is heated to 85°C to inactivate the enzyme for 10 minutes to obtain the enzymolysis solution;

[0023] (4) Ultrafiltration: The enzymatic hydrolysate is subjected to ultrafiltration through a...

Example Embodiment

[0026] Example 2

[0027] (1) Beating: Take 500 kilograms of frozen pig lungs, clean and decontaminate after natural thawing, strip the trachea, remove the grease, then use a chopper to mince it into a minced meat, and then grind and homogenize with a fine grinder for 10 minutes to obtain pig lungs Slurry

[0028] (2) Degreasing: Add 1,000 kg of water to the lung slurry, stir and mix, adjust its pH to 8.8, heat to 43-45°C, add 1200 grams of lipase, keep warm for 45 minutes and pass 100 Mesh filtering, collecting filter residue;

[0029] (3) Enzymatic hydrolysis: add water 3 times its weight to the collected filter residue, stir evenly, adjust the pH to 8.5, add 3.5 grams of alkaline protease per kilogram of filter residue, heat to 45-48°C, keep the enzyme hydrolyzed for 7 hours , The enzymolysis solution is heated to 85°C to inactivate the enzyme for 10 minutes to obtain the enzymolysis solution;

[0030] (4) Ultrafiltration: The enzymatic hydrolysate is subjected to ultrafiltration...

Example Embodiment

[0033] Example 3

[0034] (1) Beating: Take 1,000 kilograms of fresh pig lungs, clean and decontaminate, strip the trachea, and remove grease, then use a chopper to grind it into a minced meat shape, and then grind and homogenize with a fine grinder for 10 minutes to obtain a pig lung slurry ;

[0035] (2) Degreasing: Add 2500 kg of water to the lung slurry, stir and mix, adjust its pH to 9.0, heat up to 43-45°C, add 2800 grams of lipase, keep warm for 50 minutes and pass 100 Mesh filtering, collecting filter residue;

[0036] (3) Enzymatic hydrolysis: add water 2.5 times its weight to the collected filter residue, stir evenly, adjust the pH to 8.2, add 4.5 grams of alkaline protease per kilogram of filter residue, heat to 47-50°C, keep the enzyme hydrolyzed for 5 hours , The enzymolysis solution is heated to 85°C to inactivate the enzyme for 10 minutes to obtain the enzymolysis solution;

[0037] (4) Ultrafiltration: The enzymatic hydrolysate is subjected to ultrafiltration through...

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Abstract

The invention relates to a preparation method for extracting high-quality protein peptide from a pig lung. The preparation method comprises the following main steps: pulping, degreasing, performing enzymolysis, performing ultrafiltration, performing nanofiltration and performing spray drying to obtain pig lung protein peptide powder. According to the preparation method, the pig lung is taken as araw material, a biological enzymolysis method is adopted, and an ultrafiltration and nanofiltration membrane separation technology is combined; the preparation method is simple in technological process, short in production cycle, low in cost, waste-free, pollution-free, and suitable for large-scale industrial production. A prepared pig lung protein peptide product is high in quality, easy to absorb and utilize and high in nutritional value, contains polypeptides, small peptides and amino acid substances, is a high-quality functional animal protein feed raw material, and has a wide applicationprospect in the feed industry, wherein peptide substances with the molecular weight smaller than 5000Da account for 80% or more than 80% of total protein, and active oligopeptides with the molecular weight of 180-1000Da account for 60% or more than 60%.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a preparation method for extracting high-quality protein peptides from pig lungs. Background technique [0002] A large number of studies have shown that there is an absorption mechanism for small molecular peptides (oligopeptides) in animals, and small molecular peptides (oligopeptides) can enter the body circulation through intestinal mucosal cells intact. 67% of amino acids in animals are absorbed in the form of small molecular peptides (oligopeptides), and the remaining 33% are absorbed in the form of free amino acids (FAA). Small molecular peptides (oligopeptides) not only improve the nitrogen frame for protein synthesis, but also have important physiological regulatory functions: (1) promote amino acid absorption and protein synthesis speed; (2) improve the absorption and utilization of minerals; (3) improve (4) Promote the development of the digestive tract and reduce the occ...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/34
CPCC12P21/06C07K1/34
Inventor 陈石良
Owner 陈石良
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