Apple V-ATPase subunit gene MdVHA-B1S396A and stress-resistance application thereof

A mdvha-b1s396a, apple technology, applied in application, genetic engineering, plant genetic improvement and other directions, can solve the problem of long cycle of resistant varieties
CN104531718AActive Publication Date: 2015-04-22SHANDONG AGRICULTURAL UNIVERSITY
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Patent Information

Authority / Receiving Office
CN Β· China
Patent Type
Applications(China)
Current Assignee / Owner
SHANDONG AGRICULTURAL UNIVERSITY
Publication Date
2015-04-22

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Abstract

The invention relates to apple V-ATPase subunit gene MdVHA-B1S396A and stress-resistance application thereof. An MdVHA-B1 gene is separated from an apple; then, a serine residue at the 396 position is mutated into alanine through carrying out a PCR (Polymerase Chain Reaction) bridging method for two times so as to obtain the MdVHA-B1S396A gene; the expression level of transgenic apple healing can be further increased through over-expression of the gene in apple tree healing; and simultaneously, the adversity ability of transgenic apple healing, such as salinity resistance and the like, can be further increased.
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Description

1. Technical field

[0001] The present invention relates to an apple V-ATPase subunit gene MdVHA-B1S396A and its anti-stress application, in particular to the construction of a carrier of the apple V-ATPase subunit gene MdVHA-B1S396A and its ability to resist salt stress in transgenic apple callus Analysis and application, belonging to the field of molecular biology and biotechnology. 2. Background technology

[0002] Plants are often attacked by various adverse environments and pests throughout their lives. During the long-term adaptation and evolution process, plants have formed a series of defense response mechanisms to resist damage from adverse environments. Among them, environmental stress mainly includes various biotic stress and abiotic stress. Biological stress mainly comes from insects, herbivores, and plant pathogens; there are many types of abiotic stress, including drought, salinity, extreme temperature, chemical pollution, and oxygen damage. In order to adapt ...

Claims

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