The invention discloses tuber melanosporum, cordyceps and rhizoma polygonati granules and a preparation method thereof, and relates to the technical field of health care products. The raw materials ofthe tuber melanosporum, cordyceps and rhizoma polygonati granules comprise main materials and auxiliary materials, wherein the main materials include the following ingredients in parts by weight: 3-6parts of tuber melanosporum extracts, 3-6 parts of cordyceps powders, 12-16 parts of stichopus japonicas extracts, 12-16 parts of cervus nippon penis et testis cervi extracts, 7-10 parts of Maca powders, 10-15 parts of radix morindae officinalis extracts, 10-15 parts of rhizoma polygonati extracts, 10-15 parts of prepared radix rehmanniae extracts and 18-25 parts of glycyrrhizae radix extracts. In the tuber melanosporum, cordyceps and rhizoma polygonati granules, the glycyrrhizae radix is put in an important position, rhizoma polygonati is taken as an auxiliary material, the efficacy of warming the middle and benefiting qi of the rhizoma polygonati is taken, the stichopus japonicas is assisted to nourish the blood, nourish yin and replenish yin fluid, then, ingredients, including the tuber melanosporum extracts, the cordyceps powders, the cervus nippon penis et testis cervi extracts, the Maca powders, the radix morindae officinalis extracts the prepared radix rehmanniae extracts and the like, are added to nourish yin, supplement the kidney and enhance a human body autoimmunity function, the internal environment of the human body is maintained, physical power is accelerated to recover, the own anti-fatigue ability of the human body is improved, and the tuber melanosporum, cordyceps and rhizoma polygonati granules can be used for health care and body nourishment.