Trichoderma asperellum chlamydospore powder as well as preparation method thereof and application

A spore powder, Trichoderma technology, applied in the field of microorganisms, can solve problems such as not yet seen

Active Publication Date: 2014-04-23
INST OF ENVIRONMENT & SUSTAINABLE DEV IN AGRI CHINESE ACADEMY OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there are no relevant reports on the preparation of Trichoderma aculeatus c...

Method used

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  • Trichoderma asperellum chlamydospore powder as well as preparation method thereof and application
  • Trichoderma asperellum chlamydospore powder as well as preparation method thereof and application
  • Trichoderma asperellum chlamydospore powder as well as preparation method thereof and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0096] Embodiment 1: preparation trichoderma aculeatus chlamydospore powder

[0097] 1. Preparation of culture medium

[0098] (1) Preparation of strain culture medium (PGP): Take 20g peeled potatoes and boil in water for 20 minutes, squeeze the juice, mix the obtained juice with 1.0g glucose, 0.3g peptone, 2.0g agar and 100ml water, mix the obtained The pH value of the mixture is adjusted to 6.8-7.2, and the bacteria culture medium is obtained after sterilization to obtain the bacteria culture medium.

[0099] (2) Preparation of arsenic-containing PGP medium: Sodium arsenate was dissolved in 100 ml of the above-mentioned PGP medium to obtain the arsenic-containing PGP medium.

[0100] (3) Preparation of shake flask seed medium: mix 2.5g starch, 1.2g glucose, 1.2g yeast powder and 0.2g CaCO 3 Dissolve in 100 parts by weight of water, set the natural pH value, and then carry out moist heat sterilization to obtain the shake flask seed culture.

[0101] (4) Preparation of ferm...

Embodiment 2

[0118] Embodiment 2: preparation trichoderma aculeatus chlamydospore powder

[0119] 1. Preparation of culture medium

[0120] In this example, various media were prepared in the same manner as in Example 1.

[0121] Two, the preparation of solid chlamydospore powder

[0122] The preparation steps are as follows:

[0123] A. Arsenic-resistant activity maintenance culture

[0124] According to the volume ratio of Trichoderma aculeatus (CGMCC No.3186) bacterial suspension to bacterial culture medium 1:50, the bacterial content was 7.8×10 4Cfu / ml of Trichoderma aculeatus suspension was inserted into the culture medium with arsenic concentration of 45mg / l in the culture bottle, cultured continuously for 5 days at a temperature of 28°C and a shaker speed of 120rpm, and waited for When the volume of Trichoderma aculeatus mycelia in the bottle is 3 / 5 of the volume of the culture medium for the strain, the suspension of Trichoderma aculeatus is taken out from the bottle and carrie...

Embodiment 3

[0136] Embodiment 3: preparation Trichoderma aculeatus chlamydospore powder

[0137] 1. Preparation of culture medium

[0138] In this example, various media were prepared in the same manner as in Example 1.

[0139] Two, the preparation of solid chlamydospore powder

[0140] The preparation steps are as follows:

[0141] A. Arsenic-resistant activity maintenance culture

[0142] According to the volume ratio of Trichoderma aculeatus (CGMCC No.3186) bacterial suspension to bacterial culture medium 1:55, the bacterial content was 1.0×10 4 cfu / ml of Trichoderma aculeatus suspension was inserted into the culture medium with arsenic concentration of 48 mg / l in the culture bottle, and continuously cultured for 6 days at a temperature of 30 °C and a shaker speed of 130 rpm, and waited for When the volume of Trichoderma aculeatus mycelia in the bottle is 1 / 2 of the volume of the culture medium, the suspension of Trichoderma aculeatus is taken out from the bottle and carried out u...

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Abstract

The invention relates to trichoderma asperellum chlamydospore powder as well as a preparation method thereof and an application. The preparation method of the trichoderma asperellum chlamydospore powder comprises the following steps: conservatively culturing arsenic-resisting activity, purifying, culturing shake-flask seeds, culturing fermented seeds, controlling the fermentation and post-processing. The trichoderma asperellum chlamydospore is high in adversity resistance, the powder of the trichoderma asperellum chlamydospore is slightly subjected to the ambient temperature, acidicity, alkalinity and the like, and the influence of different arsenic concentrations in the environment on the sprouting of the chlamydospore is not obvious. The trichoderma asperellum chlamydospore has strong vitality, the survival time of the trichoderma asperellum chlamydospore is long, the volatilization of arsenic in the soil can be obviously promoted, the total quantity of the arsenic in the soil can be reduced, and the soil polluted by the arsenic can be effectively repaired. By adopting the method, the time for producing a great amount of trichoderma asperellum chlamydospore can be greatly shortened, the production cost of the trichoderma asperellum chlamydospore can be remarkably reduced, and the popularization of the technology can be favored.

Description

【Technical field】 [0001] The invention belongs to the technical field of microorganisms. More specifically, the present invention relates to a Trichoderma aculeatus chlamydospore powder, a method for preparing the Trichoderma aculeatus chlamydospore powder, and an application of the Trichoderma aculeatus chlamydospore powder. 【Background technique】 [0002] Trichoderma spp. is a kind of saprophytic fungus ubiquitous in soil, with fast growth and large biomass. Trichoderma can produce three kinds of propagules during its growth cycle, including mycelium, chlamydospore and conidia. At present, most of the commonly used Trichoderma agents in actual production are its live conidia preparations, such as the one developed by Israel. Trichodex wettable powder of Trichoderma harzianum T39, Topshield (Trichoderma harzianum T22) of the United States, etc. Some Trichoderma can often form round or oval chlamydospores, which grow interstitially on the hyphae in the base, or grow at the...

Claims

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Application Information

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IPC IPC(8): C12N3/00B09C1/10C12R1/885
Inventor 曾希柏蒋细良苏世鸣王秀荣李莲芳白玲玉王亚男段然吴翠霞
Owner INST OF ENVIRONMENT & SUSTAINABLE DEV IN AGRI CHINESE ACADEMY OF AGRI SCI
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