Preparation method of goat milk containing ACE (Angiotensin Converting Enzyme) inhibitory peptide based on Lactobacillus helveticus fermentation
A technology that inhibits peptides and lactobacillus, applied in the field of goat milk preparation, can solve the problems of consumption of medical and social resources, heavy national burden, high mortality rate, etc., to enhance immunity, the method is simple and easy to implement, and prevent the onset of hypertension Effect
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[0028] Example 1
[0029] The preparation method of goat milk containing ACE inhibitory peptide based on fermentation of Lactobacillus helveticus includes the following steps:
[0030] 1) Inoculate 0.4g of freeze-dried bacterial powder of Lactobacillus helveticus in an anaerobic tube containing 10mL of sterilized goat milk reconstituted milk, mix well and incubate at 37°C for 12 hours to complete the first activation; take 5% of the above For the first activated bacteria liquid, perform the second and third activations according to the above-mentioned operation method, and activate a total of 3 times to obtain the activated bacteria.
[0031] 2) Add 10mL of 1% casein solution to 90mL of fresh goat milk and mix well, filter and purify, sterilize at 90℃ for 15min, cool to 40±3℃, insert 5mL activated Lactobacillus helveticus Anaerobic fermentation at 37°C for 12 hours to obtain fermented goat milk.
[0032] 3) Based on parts by mass, dissolve 5.7 parts of sucrose and 0.3 parts of pectin...
Example Embodiment
[0041] Example 2
[0042] The preparation method of goat milk containing ACE inhibitory peptide based on fermentation of Lactobacillus helveticus includes the following steps:
[0043] 1) Inoculate 0.4g of freeze-dried bacterial powder of Lactobacillus helveticus in an anaerobic tube containing 10mL of sterilized goat milk reconstituted milk, mix well and incubate at 37°C for 12 hours to complete the first activation; take 5% of the above For the first activated strain, perform the second activation according to the above operation method, and activate 3 times in total to obtain the activated strain.
[0044] 2) Add 14g of goat milk powder and 20mL of 1% casein solution to 80mL of water and mix well, sterilize at 90℃ for 15min, cool to 40±3℃, insert 7mL of activated Lactobacillus helveticus, Anaerobic fermentation at a constant temperature of 40°C for 10 hours to obtain fermented goat milk.
[0045] 3) In parts by mass, dissolve 7 parts of sucrose and 0.2 parts of sodium carboxymethy...
Example Embodiment
[0047] Example 3
[0048] The preparation method of goat milk containing ACE inhibitory peptide based on fermentation of Lactobacillus helveticus includes the following steps:
[0049] 1) Inoculate 0.4g of freeze-dried bacterial powder of Lactobacillus helveticus in an anaerobic tube containing 10mL of sterilized goat milk reconstituted milk, mix well and incubate at 37°C for 12 hours to complete the first activation; take 5% of the above For the first activated strain, perform the second activation according to the above operation method, and activate 3 times in total to obtain the activated strain.
[0050] 2) Add 14g goat milk powder and 50mL 1% casein solution to 50mL water and mix well, sterilize at 90℃ for 15min, cool to 40±3℃, insert 7mL activated Lactobacillus helveticus, Constant temperature anaerobic fermentation at 35°C for 14 hours to obtain fermented goat milk.
[0051] 3) In terms of parts by mass, dissolve 5 parts of sucrose and 0.3 parts of propylene glycol alginate i...
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