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Preparation method of goat milk containing ACE (Angiotensin Converting Enzyme) inhibitory peptide based on Lactobacillus helveticus fermentation

A technology that inhibits peptides and lactobacillus, applied in the field of goat milk preparation, can solve the problems of consumption of medical and social resources, heavy national burden, high mortality rate, etc., to enhance immunity, the method is simple and easy to implement, and prevent the onset of hypertension Effect

Inactive Publication Date: 2013-03-13
SHAANXI UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Hypertension can cause various complications such as stroke, myocardial infarction, heart failure and chronic kidney disease, which not only causes disability and high mortality rate, but also seriously consumes medical and social resources, placing a heavy burden on the family and the country

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The preparation method of goat milk containing ACE inhibitory peptides fermented by Lactobacillus helveticus comprises the following steps:

[0030] 1) Inoculate 0.4g of freeze-dried Lactobacillus helveticus powder into an anaerobic tube containing 10mL of sterilized goat milk reconstituted milk, mix well and incubate at 37°C for 12 hours to complete the first activation; take 5% of the above The bacteria liquid activated for the first time was activated for the second and third times according to the above-mentioned operation method, and the activated bacteria were obtained for a total of 3 times.

[0031] 2) Add 10mL of 1% casein solution to 90mL of fresh goat milk and mix well, filter and purify, sterilize at 90°C for 15min, cool to 40±3°C, add 5mL of activated Lactobacillus helveticus , fermented anaerobically at a constant temperature of 37° C. for 12 hours to obtain fermented goat milk.

[0032] 3) In terms of parts by mass, dissolve 5.7 parts of sucrose and 0.3 ...

Embodiment 2

[0042] The preparation method of goat milk containing ACE inhibitory peptides fermented by Lactobacillus helveticus comprises the following steps:

[0043] 1) Inoculate 0.4g of freeze-dried Lactobacillus helveticus powder into an anaerobic tube containing 10mL of sterilized goat milk reconstituted milk, mix well and incubate at 37°C for 12 hours to complete the first activation; take 5% of the above The bacterial strain activated for the first time is activated for the second time according to the above operation method, and the activated bacterial strain is obtained for a total of 3 times.

[0044] 2) Add 14g of goat milk powder and 20mL of 1% casein solution to 80mL of water and mix well, sterilize at 90°C for 15min, cool to 40±3°C, insert 7mL of activated Lactobacillus helveticus, Anaerobic fermentation at a constant temperature of 40°C for 10 hours to obtain fermented goat milk.

[0045] 3) In terms of parts by mass, dissolve 7 parts of sucrose and 0.2 parts of sodium car...

Embodiment 3

[0048] The preparation method of goat milk containing ACE inhibitory peptides fermented by Lactobacillus helveticus comprises the following steps:

[0049] 1) Inoculate 0.4g of freeze-dried Lactobacillus helveticus powder into an anaerobic tube containing 10mL of sterilized goat milk reconstituted milk, mix well and incubate at 37°C for 12 hours to complete the first activation; take 5% of the above The bacterial strain activated for the first time is activated for the second time according to the above operation method, and the activated bacterial strain is obtained for a total of 3 times.

[0050] 2) Add 14g of goat milk powder and 50mL of 1% casein solution into 50mL of water and mix evenly, sterilize at 90°C for 15min, cool to 40±3°C, insert 7mL of activated Lactobacillus helveticus, in Anaerobic fermentation at constant temperature at 35°C for 14 hours to obtain fermented goat milk.

[0051] 3) In terms of parts by mass, dissolve 5 parts of sucrose and 0.3 parts of propy...

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Abstract

The invention discloses a preparation method of goat milk containing ACE (Angiotensin Converting Enzyme) inhibitory peptide based on Lactobacillus helveticus fermentation. The preparation method comprises the following steps of: vaccinating Lactobacillus helveticus in goat milk or goat milk reconstituted milk, and carrying out anaerobic fermentation at a temperature of 30-40 DEG C for 10-15 hours, so as to obtain fermented goat milk; sufficiently mixing 80-90 parts of the fermented goat milk, 5-10 parts of sucrose, 0.15-0.5 parts of a stabilizer and 5-30 parts of water and then cooling to a temperature below 10 DEG C, so as to obtain mixed fermented goat milk; and sealing the mixed fermented goat milk at a temperature of 2-5 DEG C, and maturating for 8-10 hours so as to obtain the goat milk containing the ACE inhibitory peptide. The prepared goat milk has nutritive values of goat yogurt and specific tastes, and further contains the ACE inhibitory peptide with higher inhibition rate, so that not only can the development and the utilization of the goat milk resources be prompted, but also the goat milk has positive effects on hypertension prevention and nutrition and health care of a patient suffering from hypertension.

Description

technical field [0001] The invention belongs to the technical field of goat milk fermentation, and relates to a method for preparing goat milk containing ACE inhibitory peptides fermented by Lactobacillus helveticus. Background technique [0002] The World Health Organization (WHO) defines hypertension as: systolic blood pressure ≥ 140 mmHg and / or diastolic blood pressure ≥ 90 mmHg when blood pressure is measured three times on different days without using antihypertensive drugs. According to the survey data on nutrition and health status of 270,000 national residents organized by the Ministry of Health in 2010, the prevalence rate of hypertension among people aged 60 and over in my country is 49%, that is, about 1 out of every 2 people over 60 years old. Man suffers from high blood pressure. In recent years, the prevalence of hypertension in children has also increased. According to survey results in some provinces and cities in the past 10 years, the prevalence of hyperten...

Claims

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Application Information

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IPC IPC(8): A23C9/123A23L1/29A61K38/02A61P9/12A23L33/00
Inventor 陈合吉喆舒国伟
Owner SHAANXI UNIV OF SCI & TECH
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