Method for preparing laminarin from kelp

The technology of kelp polysaccharide and kelp is applied in the field of polysaccharide preparation, can solve the problems of restricting the deep processing and application of lamina polysaccharide, reducing the biological activity of polysaccharide, time-consuming enzyme extraction method, etc., and achieving high-efficiency cell wall breaking, promoting decomposition and operation simple effect

Inactive Publication Date: 2015-06-17
广西还珠海洋生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are some documents or reports on the extraction of laminaria polysaccharides. The traditional methods are mainly acid extraction, alkali extraction, enzyme extraction, etc. These extraction methods have certain defects: acid extraction can cause polysaccharides Hydrolysis and alkali extraction lead to a decrease in the biological activity of polysaccharides. Enzyme extraction is time-consuming. Industrial production requires a large amount of enzymes and high costs. The method of hot water extraction and alcohol precipitation is simple, but time-consuming and energy-consuming
There are deficiencies in the existing methods, which restrict the deep processing and application of laminarin to a certain extent

Method used

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  • Method for preparing laminarin from kelp

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Effect test

Embodiment 1

[0026] A method for preparing laminarin from kelp, comprising the steps of:

[0027] 1) Raw material processing: After the kelp is dried, it is pulverized;

[0028] 2) High-pressure micro-jet micro-grinding treatment: Add pure water to the pulverized kelp according to the weight ratio of 1:8, process it with a high-speed shear emulsifier for 10 minutes at a speed of 12000r / min, and then use high-pressure micro-jet ultra-fine pulverization The equipment is processed at 25°C, the processing pressure is 80MPa, and the processing times are 3 times to obtain a paste slurry;

[0029] 3) Fermentation: The paste slurry obtained in step 2) is subjected to ultra-high temperature instantaneous sterilization, then adjusted to pH 7.0, inserted into the cultured mature Lactobacillus bulgaricus liquid strain at 10% by volume, and put into a fermenter for fermentation , the fermentation temperature is 35-37°C, and the fermentation time is 3h;

[0030] 4) Enzymolysis: The fermented liquid ob...

Embodiment 2

[0034] A method for preparing laminarin from kelp, comprising the steps of:

[0035] 1) Raw material processing: After the kelp is dried, it is pulverized;

[0036] 2) High-pressure micro-jet micro-grinding treatment: Add pure water to the pulverized kelp according to the weight ratio of 1:10, process it with a high-speed shear emulsifier for 15 minutes at a speed of 10000r / min, and then use high-pressure micro-jet ultra-fine pulverization The equipment is processed at 25°C, the processing pressure is 100MPa, and the processing times are 2 times to obtain a paste slurry;

[0037] 3) Fermentation: The paste slurry obtained in step 2) is subjected to ultra-high temperature instantaneous sterilization, then adjusted to pH 6.5, and inserted into a cultured mature Lactobacillus acidophilus liquid strain at 12% by volume, and put into a fermenter Fermentation, the fermentation temperature is 35-37°C, and the fermentation time is 4h;

[0038] 4) Enzymolysis: The fermented liquid ob...

Embodiment 3

[0042] A method for preparing laminarin from kelp, comprising the steps of:

[0043] 1) Raw material processing: After the kelp is dried, it is pulverized;

[0044] 2) High-pressure micro-jet micro-grinding treatment: Add pure water to the pulverized kelp according to the weight ratio of 1:8, process it with a high-speed shear emulsifier for 20 minutes at a speed of 8000r / min, and then use high-pressure micro-jet ultra-fine pulverization The equipment is processed at 25°C, the processing pressure is 120MPa, and the processing times are 3 times to obtain a paste slurry;

[0045] 3) Fermentation: The paste slurry obtained in step 2) is subjected to ultra-high temperature instantaneous sterilization, then adjusted to pH 7.0, inserted into the mature liquid strain of Bifidobacterium infantis by volume percentage of 15%, and put into a fermenter Fermentation, the fermentation temperature is 35-37°C, and the fermentation time is 3h;

[0046]4) Enzymolysis: The fermented liquid obt...

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Abstract

The invention discloses a method for preparing laminarin from kelp, which comprises the following steps: raw material treatment, high-pressure microjet pulverization treatment, fermentation, enzymolysis, decolorization, protein removal and drying to obtain the finished product. Compared with the existing trichloroacetic acid precipitation process, savage process or the like, the method disclosed by the invention has the advantages of high laminarin yield and high content.

Description

technical field [0001] The invention relates to a method for preparing polysaccharides, in particular to a method for preparing laminaria polysaccharides from kelp. Background technique [0002] Kelp (Laminaria Japonica Aresch) is a large seaweed belonging to the genus Laminaria of the family Laminaria. Kelp is rich in a variety of functional components and has extremely high edible and medicinal value. Its medicinal value has been recorded in traditional medical books such as "Compendium of Materia Medica", "Shen Nong's Materia Medica" and "Food Materia Medica". Studies have shown that the various biological functions of kelp are mostly related to the physiologically active polysaccharides in kelp, such as immune regulation, anti-tumor, anti-coagulation, lowering lipids, lowering blood sugar, and anti-oxidation. [0003] At present, there are some documents or reports on the extraction of laminaria polysaccharides. The traditional methods are mainly acid extraction, alkali...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 归三岷
Owner 广西还珠海洋生物科技有限公司
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