Method for extracting and separating laminarin with low molecular weight and application thereof in cigarette products

A separation method and technology of kelp polysaccharides, which are applied in the field of kelp deep processing, can solve the problems of improving sensory properties, etc., and achieve the effects of improving smoking quality, delicate aroma, and soft smoke

Inactive Publication Date: 2012-06-27
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Tobacco is a special consumer product. Smokers mainly obtain physical and psychological satisfaction by smoking the smoke produced by burning tobacco. Therefore, the sensory characteristics

Method used

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  • Method for extracting and separating laminarin with low molecular weight and application thereof in cigarette products
  • Method for extracting and separating laminarin with low molecular weight and application thereof in cigarette products
  • Method for extracting and separating laminarin with low molecular weight and application thereof in cigarette products

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] (1) The kelp is desanded and washed, dried and crushed, and passed through a 40-mesh sieve to obtain the extracted raw material;

[0026] (2) Add an acetic acid solution with a pH value of 2 to the raw material for extraction, add 35 times the weight of the dry kelp powder, extract at 115°C for 4 hours, filter, separate the kelp slag to obtain the extract, and adjust the pH of the extract with potassium hydroxide value to 7.0;

[0027] (3) Vacuum-concentrate the extract to 1 / 5 of the original volume to obtain a concentrate;

[0028] (4) Add ethanol to the concentrated solution, after mixing evenly, the final concentration of ethanol is 80% (v / v), let stand at 2°C for 8 hours, filter, remove the supernatant part, and polysaccharides precipitate;

[0029] (5) The precipitate was collected and freeze-dried to obtain laminarin 1# with a molecular weight of 20711 Da, which was light brown.

[0030] The sample was added to cigarettes in an amount of 0.1% of the mass of shre...

Embodiment 2

[0032] (1) The kelp is desanded and washed, dried and crushed, and passed through a 20-mesh sieve to obtain the extracted raw material;

[0033] (2) Add an acetic acid solution with a pH value of 3 to the extraction raw material, add 40 times the weight of the dry kelp powder, extract at 125°C for 2 hours, filter, separate the kelp slag to obtain the extract, and adjust the pH of the extract with potassium hydroxide value to 7.0;

[0034] (3) Perform ultrafiltration on the extract obtained in step (2) with a membrane with a molecular weight cut-off of 10,000 Daltons, and collect the permeate;

[0035] (4) Vacuum concentrate the ultrafiltration permeate to 1 / 6 of the original volume to obtain a concentrate;

[0036] (5) Add ethanol to the concentrated solution, after mixing evenly, the final concentration of ethanol is 85% (v / v), let it stand at 0°C for 6 hours, filter, remove the supernatant part, and polysaccharides precipitate;

[0037] (6) The precipitate was collected an...

Embodiment 3

[0040] (1) The kelp is desanded and washed, dried and crushed, and passed through a 40-mesh sieve to obtain the extracted raw material;

[0041] (2) Add a citric acid solution with a pH value of 2 to the kelp raw material, add 30 times the weight of the dry kelp powder, extract at 120°C for 3 hours, filter, separate the kelp slag to obtain the extract, and adjust the extract with potassium hydroxide pH to 6.5;

[0042] (3) Perform ultrafiltration on the extract obtained in step (2) with a membrane with a molecular weight cut-off of 10,000 Daltons, and collect the permeate;

[0043] (4) Vacuum concentrate the ultrafiltration permeate to 1 / 5 of the original volume to obtain a concentrate;

[0044] (5) Add ethanol to the concentrated solution, after mixing evenly, the final concentration of ethanol is 80% (v / v), let it stand at 4°C for 12 hours, filter, remove the supernatant part, polysaccharides precipitate, and use ethanol and acetone Soak 2-3 times;

[0045] (6) The precip...

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Abstract

The invention discloses a method for extracting and separating laminarin with low molecular weight and application thereof in cigarette products. The laminarin with the low molecular weight organic acid is extracted by adopting an organic acid method. After kelps are smashed, the laminarin with the low molecular weight organic acid is obtained through organic acid extraction, ultra-filtration, concentration and ethanol fractional precipitation and separation, the molecular weight of the laminarin is less than 10000 Dalton, and solid powder is light brown. The method for extracting and separating the laminarin with the low molecular weight is simple and suitable for industrialization production, the obtained laminarin with the low molecular weight serves as a cigarette ingredient to be applied to cigarettes, smoking quality is improved remarkably, miscellaneous gases are removed effectively, fragrance is improved and embellished to be fine and smooth, smoke is soft, smoke quality is improved obviously, stimulation to nasal cavity, throat and the like is reduced, and the laminarin has positive effect.

Description

technical field [0001] The invention relates to deep processing technology of kelp, in particular to a method for extracting and separating low-molecular-weight kelp polysaccharide and its application in tobacco, belonging to the field of food biotechnology. Background technique [0002] Kelp (Laminaria japonica), also known as kelp, is a marine plant with high edible and medicinal value. It contains a variety of active substances beneficial to the human body, including active polysaccharides, trace minerals, vitamins, etc. Radiation, lowering blood fat, lowering blood sugar, regulating immune function and other health effects. In addition, kelp is rich in resources and low in price in our country, so it is a food resource with great potential. At present, laminarin has been developed and applied in functional food, medicine, biology, materials and so on. For example, Yantai Mingyuansu Marine Biotechnology Co., Ltd. (Chinese Patent Application No. 200710015838.8) discloses...

Claims

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Application Information

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IPC IPC(8): A24B15/26A24B3/12A24D3/14
Inventor 崔春卢茳虹赵谋明游丽君
Owner SOUTH CHINA UNIV OF TECH
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