Kelp degrading bacteria and method for preparing kelp juice utilizing same

A technology of kelp and dried kelp, applied in the field of environmental microorganisms, can solve the problems of low comprehensive utilization rate of raw materials, low technical content, single variety of formed products, etc., and achieve the effect of improving comprehensive utilization rate and edible quality, and removing algae structure

Inactive Publication Date: 2014-01-29
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, compared with the advantages in breeding, the processing level of kelp in my country is far behind that of Japan and South Korea. The main performances are: 1. The comprehensive utilization rate of raw materials is low. At present, the comprehensive utilization rate of kelp in my country is still less than 30%. 2/3 of the active ingredients of kelp have not been developed, and in Japan and South Korea, where kelp processing technology is developed, the comprehensive utilization rate of kelp has reached more than 80%; 2. The variety of shaped products is single and the technical content is low. There are only dozens of types, and they are basically limited to primary proces...

Method used

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  • Kelp degrading bacteria and method for preparing kelp juice utilizing same
  • Kelp degrading bacteria and method for preparing kelp juice utilizing same
  • Kelp degrading bacteria and method for preparing kelp juice utilizing same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1: Strains Tamlana alginolytica Breeding of ZJU HZ22 (CGMCC No. 5324)

[0030] The strains were isolated from surface seawater and planktonic algae samples collected in Zhoushan, East China Sea in August 2010. After the algae are properly ground, put them into a sterile shaker flask together with seawater samples, and at the same time supplement sterile fresh kelp pieces (about 1.5 cm×1.5 cm, 10 pieces) and peptone / The mixed concentrated solution of yeast extract (the final concentration is 1 g / l) was cultivated in an air bath shaker (28°C, 120 rpm), and the turbidity of the enriched solution and the changes of kelp pieces were observed regularly. After the enrichment solution was turbid, select the positive shake flask (that is, the kelp pieces are broken or become smaller), and transfer to oligotrophic 2216 medium (containing 200 g / l of fresh kelp pieces) according to 10% inoculum amount, and in the same condition The culture was continued under the...

Embodiment 2

[0037] Example 2: Strains Tamlana alginolytica Analysis of extracellular degrading enzymes of ZJU HZ22 (CGMCC No. 5324)

[0038] Qualitative analysis on plates and quantitative analysis on shake flasks were used respectively.

[0039] Qualitative analysis of plates: Based on oligotrophic 2216 medium, add 1% sodium alginate, soluble starch, sodium carboxymethylcellulose and 0.2% pectin to prepare plates, and scrape an appropriate amount of fresh bacteria from the 2216 slope The soil was planted, and the enzyme activity was tested after culturing at 28°C until an obvious bacterial lawn was formed. Among them, amylase was stained with Lugol's iodine solution, and a transparent circle around the bacterial lawn was positive; cellulase and pectinase were spread and stained with 0.2% Congo red solution, and a transparent circle around the bacterial lawn was positive; The lyase was stained with freshly prepared 2% calcium chloride solution, and a transparent circle around the bacte...

Embodiment 3

[0042] Embodiment 3: the bacterium preparation technology of kelp juice

[0043] Take the common dry kelp bought in the market, soak it properly, cut it into pieces (about 1.5 cm×1.5 cm), and dry it for later use.

[0044] The seed medium is a degradation medium containing 10 g / l dried kelp pieces, with a liquid volume of 200 ml / 250 ml, cultured at 28°C and 120 rpm for about 4 days, and the degree of degradation of the kelp pieces can be used to judge whether the seed liquid is Mature. The seed liquid without obvious kelp block residue was transferred to the fermenter according to the inoculum amount of 1%.

[0045] Add 4.5 L of degradation medium to a 7.5 L fermenter, add 40 g of dried kelp pieces, 2.5 ml of polyether (defoamer), and sterilize at 121 °C for 20 min. After inoculation after sufficient cooling, the automatic control parameters were set as follows: temperature at 28°C, stirring speed at 300 rpm, and ventilation at 2.5 LPM. Sampling at regular intervals and...

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Abstract

The invention provides a kelp degrading strain having kelp degrading activity. The strain is conserved in China General Microbiological Culture Collection Center, is classified and named as Tamlana alginolytica, and has the conservation number of CGMCC No. 5324. The provided strain is capable of generating an extracellular compound enzyme system having the activity of degrading various polysaccharides and also capable of realizing deep degradation of kelp. The invention provides two kelp juice preparation processes without adding an extracting medium and without heating and heat preservation assistance, namely a bacterial treatment process with kelp pieces as the raw material and an enzymatic treatment process with kelp powder as the raw material; the two processes both are capable of degrading kelp polysaccharides into reducing oligosaccharides or monosaccharides through biodegradation under mild conditions, and simultaneously achieving the effect of deodorization. According to the invention, the comprehensive utilization rate and eating quality of the raw material are improved and technical support is provided for refined and deep processing of kelp; as a result, the kelp degrading strain has enormous application prospect in the development aspect of kelp beverage.

Description

technical field [0001] The invention belongs to the technical field of environmental microorganisms, in particular to a strain of marine bacteria Tamlana alginolytica ZJU HZ22, strain preservation number is CGMCC No. 5324, and its application in biological preparation of kelp juice. Background technique [0002] Kelp farming is a characteristic mariculture industry in the Northwest Pacific Ocean, mainly including China, Japan, South Korea and other countries. At present, the annual output of kelp in my country has reached more than 800,000 tons (dry weight), ranking first in the world. The scale of farming is only about 200,000 to 100,000 tons (dry weight) per year. However, compared with the advantages in breeding, the processing level of kelp in my country is far behind that of Japan and South Korea. The main performances are: 1. The comprehensive utilization rate of raw materials is low. At present, the comprehensive utilization rate of kelp in my country is still less t...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23L1/337C12R1/01A23L17/60
Inventor 张心齐吴敏孙聪
Owner ZHEJIANG UNIV
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