Kelp degrading bacteria and method for preparing kelp juice utilizing same

Abstract

The invention provides a kelp degrading strain having kelp degrading activity. The strain is conserved in China General Microbiological Culture Collection Center, is classified and named as Tamlana alginolytica, and has the conservation number of CGMCC No. 5324. The provided strain is capable of generating an extracellular compound enzyme system having the activity of degrading various polysaccharides and also capable of realizing deep degradation of kelp. The invention provides two kelp juice preparation processes without adding an extracting medium and without heating and heat preservation assistance, namely a bacterial treatment process with kelp pieces as the raw material and an enzymatic treatment process with kelp powder as the raw material; the two processes both are capable of degrading kelp polysaccharides into reducing oligosaccharides or monosaccharides through biodegradation under mild conditions, and simultaneously achieving the effect of deodorization. According to the invention, the comprehensive utilization rate and eating quality of the raw material are improved and technical support is provided for refined and deep processing of kelp; as a result, the kelp degrading strain has enormous application prospect in the development aspect of kelp beverage.

Description

technical field [0001] The invention belongs to the technical field of environmental microorganisms, in particular to a strain of marine bacteria Tamlana alginolytica ZJU HZ22, strain preservation number is CGMCC No. 5324, and its application in biological preparation of kelp juice. Background technique [0002] Kelp farming is a characteristic mariculture industry in the Northwest Pacific Ocean, mainly including China, Japan, South Korea and other countries. At present, the annual output of kelp in my country has reached more than 800,000 tons (dry weight), ranking first in the world. The scale of farming is only about 200,000 to 100,000 tons (dry weight) per year. However, compared with the advantages in breeding, the processing level of kelp in my country is far behind that of Japan and South Korea. The main performances are: 1. The comprehensive utilization rate of raw materials is low. At present, the comprehensive utilization rate of kelp in my country is still less t...

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Problems solved by technology

However, compared with the advantages in breeding, the processing level of kelp in my country is far behind that of Japan and South Korea. The main performances are: 1. The comprehensive utilization rate of raw materials is low. At present, the comprehensive utilization rate of kelp in my country is still less than 30%. 2 / 3 of the active ingredients of kelp have not been developed, and in Japan and South Korea, where kelp processing technology is developed, the comprehensive utilization rate of kelp has reached more than 80%; 2. The variety of shaped products is single and the technical content is low. There are only dozens of types, and they are basically limited to primary processed products such as kelp knots and kelp shreds. In Japan, more than 130 series and more than 300 varieties of kelp food and health products have been formed.

In 2007, Hu Zhihe and others studied and reported the method of enzymatic hydrolysis to prepare kelp juice, that is, the kelp powder was processed by adding cellulase, pectinase and papain, and then extracted with water to obtain kelp juice. It is the first and only report in China to use enzymatic hydrolysis technology to prepare kelp juice, but it did not enzymatically treat the alginate with the highest content in kelp. The important reason is that there are very few alginate lyase preparations on the market. extremely expensive

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