Preparation method of laminarin with anti-tumor activity

An anti-tumor activity, laminarin technology, applied in the field of kelp polysaccharide, can solve the problems of restricting the deep processing and application of kelp polysaccharide, less research, etc., and achieve the effects of avoiding the decrease of laminarin content, high protein removal rate, and avoiding changes.

Inactive Publication Date: 2015-04-01
GUILIN HEYINXIANG NEW MATERIALS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In recent years, although scholars at home and abroad have studied the extraction, separation and purification of laminar polysaccharides and made some progress, there

Method used

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  • Preparation method of laminarin with anti-tumor activity

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A preparation method of laminarin with antitumor activity, comprising the steps of:

[0023] 1) Raw material processing: 10 kg of dried sea-tangle is pulverized;

[0024] 2) Ultra-high pressure treatment: add 400kg of pure water to the pulverized kelp, pressure 350MPa, temperature 85°C, ultra-high pressure treatment for 4 minutes, filter after completion, and collect the filtrate;

[0025] 3) Ultrasonic treatment: The filtrate obtained in step 2) was ultrasonically treated for 10 minutes at an operating temperature of 85°C and a power of 300W. After completion, filter and collect the filtrate;

[0026] 4) Removal of alginate: adjust the pH value of the filtrate obtained in step 3) to 1.2 with a hydrochloric acid solution with a concentration of 1.5M, centrifuge at a speed of 12000r / min for 20min, and collect the supernatant;

[0027] 5) Decolorization: Add 95% ethanol with a mass concentration equivalent to 2 times the weight of the supernatant to the supernatant obtai...

Embodiment 2

[0030] 1) Raw material processing: crush 10kg of fresh kelp;

[0031] 2) Ultra-high pressure treatment: add 600kg of pure water to the crushed kelp, pressure 350MPa, action temperature 80°C, ultra-high pressure treatment for 2min, filter after completion, and collect the filtrate;

[0032] 3) Ultrasonic treatment: The filtrate obtained in step 2) was ultrasonically treated for 8 minutes at an operating temperature of 80°C and a power of 300W. After completion, filter and collect the filtrate;

[0033] 4) Removal of alginate: adjust the pH value of the filtrate obtained in step 3) to 1.2 with a hydrochloric acid solution with a concentration of 1.5M, centrifuge at a speed of 12000r / min for 20min, and collect the supernatant;

[0034] 5) Decolorization: Add 95% ethanol with a mass concentration equivalent to 2 times the weight of the supernatant to the supernatant obtained in step 4), let stand at 4°C for 4 hours, filter, and add 0.5% of the weight of the supernatant to the supe...

Embodiment 3

[0037] 1) Raw material processing: 10 kg of dried sea-tangle is pulverized;

[0038] 2) Ultra-high pressure treatment: Add 500kg of pure water to the pulverized kelp, pressure 350MPa, temperature 70°C, ultra-high pressure treatment for 5 minutes, filter after completion, and collect the filtrate;

[0039] 3) Ultrasonic treatment: process the filtrate obtained in step 2) for 5 minutes with ultrasonic treatment at a temperature of 70° C. and a power of 300 W. After completion, filter and collect the filtrate;

[0040] 4) Removal of alginate: adjust the pH value of the filtrate obtained in step 3) to 1.2 with a hydrochloric acid solution with a concentration of 1.5M, centrifuge at a speed of 12000r / min for 20min, and collect the supernatant;

[0041] 5) Decolorization: Add 95% ethanol with a mass concentration equivalent to 2 times the weight of the supernatant to the supernatant obtained in step 4), let stand at 4°C for 4 hours, filter, and add 0.5% of the weight of the supernat...

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Abstract

The invention discloses a method for extracting laminarin with anti-tumor activity from kelp. The method comprises the following steps: raw material treatment, ultrahigh pressure treatment, ultrasonic treatment, alginate removal, decoloration, protein removal, and drying, so as to obtain the product. An experiment proves that the obtained laminarin has good anti-tumor activity.

Description

technical field [0001] The invention relates to a preparation method of polysaccharides, in particular to a method for extracting laminarin with anti-tumor activity from kelp. Background technique [0002] Kelp (Laminaria Japonica Aresch) is a large seaweed belonging to the genus Laminaria of the family Laminaria. Kelp is rich in a variety of functional components and has extremely high edible and medicinal value. Its medicinal value has been recorded in traditional medical books such as "Compendium of Materia Medica", "Shen Nong's Materia Medica" and "Food Materia Medica". Studies have shown that the various biological functions of kelp are mostly related to the physiologically active polysaccharides in kelp, such as immune regulation, anti-tumor, anti-coagulation, lowering lipids, lowering blood sugar, and anti-oxidation. [0003] Many scholars have also studied and discussed the preparation methods of laminarin with anti-tumor activity. The traditional methods are mainly...

Claims

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Application Information

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IPC IPC(8): C08B37/00
Inventor 周冠旻
Owner GUILIN HEYINXIANG NEW MATERIALS
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