Laminarin-zinc complex, and preparation and application of same

A technology of kelp polysaccharide and zinc complex, which is applied in the field of medicine, can solve problems such as inability to achieve the effect, and achieve the effects of high development value, simple preparation process, and convenient production

Inactive Publication Date: 2017-10-24
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Usually animal zinc supplementation is to use inorganic zinc

Method used

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  • Laminarin-zinc complex, and preparation and application of same
  • Laminarin-zinc complex, and preparation and application of same
  • Laminarin-zinc complex, and preparation and application of same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] 1 Purification of kelp crude polysaccharide: deproteinization by trypsin-Sevage method

[0035] (1) Dissolve 10.00g of kelp crude polysaccharide in 100mL of water and mix well to obtain a crude kelp polysaccharide solution; mix the kelp crude polysaccharide solution and 0.25% trypsin solution at a volume ratio of 1:0.4, and put it in a constant temperature water bath Heating in a water bath at 37°C, adding 0.2 times the volume of 10wt% H after 5 hours 2 o 2 The solution (pH=7.0) was reacted for 12h, and 6mL of kelp crude polysaccharide solution was added to 2mL of Sevage reagent (chloroform-n-butanol solution with a volume ratio of 4:1), vigorously shaken on a constant temperature oscillator for 20min, and then placed in a high-speed centrifuge at 3800r / Centrifuge for 1 min, the solution is layered, take out the upper layer solution and add 2 mL of Sevage reagent, repeat the above steps until no protein appears;

[0036] (2) Slowly pour a certain amount of absolute e...

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PUM

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Abstract

The invention discloses a laminarin-zinc complex, and preparation and application of same. The laminarin-zinc complex is formed through a reaction to purified laminarin and zinc at certain temperature and under certain pH value. An animal activity experiment proves that the laminarin-zinc complex can improve anti-oxidizing capability and immunoregulation capability of animal organisms, so that the laminarin-zinc complex can be used for preparing immunomodulator medicines and health-care foods and also can be used for preparing animal immunomodulator medicines.

Description

technical field [0001] The invention relates to a laminarin-zinc complex and its preparation and application, belonging to the field of medicine. Background technique [0002] Kelp and algae polysaccharides have the functions of enhancing immunity and anti-tumor. The ocean contains more than 80% of biological species and is a huge repository of biodiversity. Kelp is a kind of edible algae with wide distribution and high nutritional value, and our country is rich in kelp resources. Studies have shown that laminarin (BSP) has a significant effect on the immune function of normal and immunocompromised mice. After BSP was administered by intraperitoneal injection, the thymus and spleen index, the number of peripheral blood leukocytes, the proliferative ability of spleen T and B cells, the ability of spleen cells to produce IL-2, and the contents of serum and spleen hemolysin were measured. Variety. Results BSP 100mg / (kg·d)×10d could significantly increase the thymus and sple...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61K31/715A61P37/02A61P39/06A23L33/125A23L33/16
CPCC08B37/00A23L33/125A23L33/16A23V2002/00A61K31/715A61K36/02C08B37/0003A23V2200/324
Inventor 李清禄滕春福李宇翔吴楠
Owner FUJIAN AGRI & FORESTRY UNIV
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