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Novel beta-glucosidase for producing glucose and laminarin oligosaccharide from seaweed

一种葡萄糖苷酶、昆布寡糖的技术，应用在新型β-葡萄糖苷酶领域，能够解决大能量、高热量等问题

Pending Publication Date: 2020-10-27
KOREA UNIV RES & BUSINESS FOUND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This pretreatment process uses high heat and thus requires a lot of energy, and the cost of the enzymes used in the enzymatic glycosylation process constitutes a large part of the overall bioprocess

Method used

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  • Novel beta-glucosidase for producing glucose and laminarin oligosaccharide from seaweed
  • Novel beta-glucosidase for producing glucose and laminarin oligosaccharide from seaweed
  • Novel beta-glucosidase for producing glucose and laminarin oligosaccharide from seaweed

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0045] Overexpression and purification of Bgl1B

[0046] For obtaining proteus strains 2-40 T Genomic DNA (ATCC43961), the proteus strain 2-40 T They were cultured at 30° C. for 12 hours in a minimal medium containing 2.3 g of instant sea salt, 20 mM Tris-HCl, 2 g of glucose, 1 g of yeast extract, and 0.5 g of ammonium chloride per 1 L. Genomic DNA was extracted using a commercial DNA isolation kit (Qiagen, Valencia, CA, USA). The target gene bgl1B was amplified from genomic DNA by polymerase chain reaction (polymerase chain reaction, PCR). The primers used here were 5'-ATACATATGAATAGACTTACACTACCGCCTTCTTCTCGT-3' (forward: SEQ ID NO: 3) and 5'-ATAGCGGCCGCGCTCCTACTCGAGACAAACTCAGCAAATGC-3' (reverse: SEQ ID NO: 4). For protein purification by affinity chromatography, the nucleotide sequence of the gene encoding six histidine residues at the C-terminus was added. The PCR product and the pET21α vector were digested with NdeI and NotI, respectively, and ligated to construct the ...

example 2

[0049] Confirmation of the optimal pH and activation temperature of Bgl1B protein

[0050] In order to explore the optimal active pH of Bgl1B protein, 20mM sodium acetate (pH 4.0 to pH 6.0), 20mM sodium phosphate (pH 6.0 to pH 7.0) and 20mM Tris-HCl-NaCl (pH 7.0 to pH 9.0) buffer (containing 0.1% (w / v) cellobiose) was reacted with Bgl1B protein at 40°C for 15 minutes.

[0051] figure 2 The relative activity of Bgl1B at pH ranging from 4.0 to 9.0 is shown. Bgl1B is most active at pH 6. At pH 4 and pH 8 or higher, the activity decreased to 30% or below.

[0052] image 3 The relative activity of Bgl1B at temperatures ranging from 2°C to 60°C is shown. At pH 6, Bgl1B exhibited the highest enzymatic activity at 40 °C. At 20°C, about 83% of the enzyme activity is maintained. Furthermore, at very low temperatures (ie, 2°C to 10°C), an enzyme activity of 58% or higher was exhibited. However, at 50°C or higher, the enzyme activity sharply decreased. This indicates that the ...

example 3

[0054] Effect of metal ions on Bgl1B

[0055] In order to examine the effect of metal ions on the activity of Bgl1B protein, 10mM Mg 2+ , Ca 2+ , Mn 2+ 、Ni 2+ 、Cu 2+ , Fe 2 + and Co 2+ were added to 0.1% (w / v) cellobiose, and the relative activity of these cases was compared to that without metal ion addition.

[0056] As shown in Table 1, Mg 2+ 、Ni 2+ and Co 2+ Has no great effect on enzyme activity, but Mn 2+ Exhibited 56.2% enzyme activity. In addition, Cu 2+ and Fe 2+ A very low enzyme activity, ie 10%, was exhibited, indicating a very strong inhibitory effect.

[0057] [Table 1]

[0058] Metal ion Relative enzyme activity (%) control group 100.0±2.8 Mg 2+

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Abstract

The present invention relates to a novel beta-glucosidase for producing glucose and a laminarin oligosaccharide from seaweed. More particularly, the present invention may produce glucose from a beta-glucosidase exhibiting transglycosylase activity while showing exo-type glucanase activity against beta-glucan, as well as produce a laminarin oligosaccharide by exhibiting transglycosylase activity against laminarin under different reaction conditions.

Description

technical field [0001] The present invention relates to a novel β-glucosidase for producing glucose and lamina oligosaccharides from seaweed. Background technique [0002] With the concern about global warming, there is an increasing interest in renewable biomass for sustainable development. Lignocellulosic biomass has long been considered as a renewable biomass for the production of fermentable sugars, such as glucose, due to its abundant resources and low cost. However, it has a high lignin content and a very complex structure, and thus requires intensive pretreatment and enzymatic glycosylation, making it difficult to use. Recently, seaweed has attracted much attention as a biomass replacing lignocellulosic biomass, and is advantageous in terms of high carbohydrate content, no need for arable land, and simple structure due to almost no lignin content. [0003] Among marine algae, about 70 million tons of brown algae are harvested in the world every year. The main carboh...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/02C12P19/04C12P19/14C12P19/18C12N9/42
CPCC12P19/18C12P19/02C12P19/14C12P19/04C12Y302/01C12N9/2445C12Y302/01021
Inventor 金京宪金东玄
Owner KOREA UNIV RES & BUSINESS FOUND
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