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Preparation and detection method of magnetic microspheres with visible glucose detection function

A technology of magnetic microspheres and glucose, which is applied in the fields of biomedicine, analysis and detection, and achieves the effect of simplifying the detection method, eliminating pain and having real-time performance.

Inactive Publication Date: 2013-09-25
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem mainly solved by the present invention is to provide a preparation method of magnetic microspheres with the function of visual glucose detection and a method for visual detection of glucose by using the magnetic microspheres with the function of visual detection of glucose, so as to detect the glucose in the body fluid conveniently and quickly. Glucose concentration, which makes up for the shortcomings of the current clinical detection of glucose that requires complex instruments

Method used

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  • Preparation and detection method of magnetic microspheres with visible glucose detection function

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Use the magnetic microspheres to detect the glucose content in tears:

[0029] (1) Preparation of photonic crystal microspheres

[0030] The monodispersed 280 nm silica nanoparticles were purified by centrifugation, and the mass percentage of colloidal nanoparticles in the nanoparticle solution was adjusted to 20%. After ultrasonic dispersion, they were used as the water phase in the microfluidic control, and the external phase was used to contain surfactants. After the n-hexadecane is collected, the resulting droplets are heated and solidified, and then placed in a muffle furnace for calcination at 800°C to improve the stability of the photonic crystal microspheres.

[0031] (2) Preparation of magnetic photonic crystal hydrogel microspheres

[0032] The magnetic photonic crystal hydrogel microspheres use the photonic crystal microspheres prepared in step (1) as a template, and are obtained by a method of template replication. Add photonic crystal microspheres cont...

Embodiment 2

[0037] Example 2 Use the magnetic microspheres to detect the glucose content in urine

[0038] (1) Preparation of photonic crystal microspheres

[0039] The monodisperse 200 nm silica nanoparticles were purified by centrifugation, and the mass percentage of colloidal nanoparticles in the nanoparticle solution was adjusted to 35%. After ultrasonic dispersion, they were used as the water phase in the microfluidic control, and the external phase was made of surfactants. Silicone oil is used to collect the resulting droplets, and after they are heated and solidified, they are put into a muffle furnace for calcination at 800°C to improve the stability of the photonic crystal microspheres.

[0040] (2) Preparation of magnetic photonic crystal hydrogel microspheres

[0041] The magnetic photonic crystal hydrogel microspheres use the photonic crystal microspheres prepared in step (1) as a template, and are obtained by a method of template replication. Add photonic crystal microspheres cont...

Embodiment 3

[0046] Example 3 Use the magnetic microspheres to detect the glucose content in saliva

[0047] (1) Preparation of photonic crystal microspheres

[0048] The monodispersed 180 nm silica nanoparticles were purified by centrifugation, and the mass percentage of colloidal nanoparticles in the nanoparticle solution was adjusted to 30%. After ultrasonic dispersion, it was used as the water phase in the microfluidic control, and the external phase was made of surfactants. The n-hexadecane is collected and the resulting droplets are heated and solidified, and then placed in a muffle furnace for calcination at 800°C to improve the stability of the photonic crystal microspheres.

[0049] (2) Preparation of magnetic photonic crystal hydrogel microspheres

[0050] The magnetic photonic crystal hydrogel microspheres use the photonic crystal microspheres prepared in step (1) as a template, and are obtained by a method of template replication. Add the photonic crystal microspheres into a soluti...

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Abstract

The invention discloses a preparation method of magnetic microspheres with a visible glucose detection function. The preparation method comprises the following steps: by utilizing a photonic crystal microsphere as a template, preparing magnetic photonic crystal hydrogel microspheres with reverse opal structures through a template copy method of hydrogel. A detection method comprises the steps that: a glucose molecule identification unit is combined with the magnetic photonic crystal hydrogel microspheres, wherein the existence or number of the glucose molecules is expressed by the displacement or color of the reflection peak of the photonic crystal microsphere; a magnet is used for collecting the magnetic microspheres according to the property that the magnetic microspheres have magnetism to improve the naked eye observation accuracy of the color of the magnetic microspheres. The visible glucose detection method based on magnetic microspheres with visible glucose detection function has the advantages of high sensitivity, strong specificity, simple operation, low cost, accurate observation, and the like.

Description

technical field [0001] The invention relates to the fields of biomedicine and analysis and detection, in particular to a glucose visualization detection method. Background technique [0002] In the past 10 years, the incidence of diabetes in my country has been increasing year by year, and it has become the third lifelong disease that endangers people's health after tumors and cardiovascular diseases. The survey shows that the prevalence of diabetes in my country has reached 9.7%, that is to say, there is about 1 diabetic in every 10 adults. In the next few years, the prevalence of diabetes in my country will continue to rise. Experts predict that by 2015, my country's diabetic patients will exceed 60 million. Diabetes has caused a serious economic burden. Statistics show that diabetic patients, who account for about 5% of the population, consume nearly 10% of national health expenditures. The rapid growth of diabetes medical expenses has caused serious social problems. ...

Claims

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Application Information

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IPC IPC(8): B01J13/02G01N21/78G01N21/25
Inventor 赵远锦王洁郭刘洋李婷婷顾忠泽
Owner SOUTHEAST UNIV
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