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Hydrogel microspheres embedded with coagulation related enzyme

A technology of hydrogel microspheres and blood coagulation, which is applied to the preparation of microspheres, medical preparations containing no active ingredients, and medical preparations containing active ingredients. Problems such as limited drug dosage and limited product application range, etc., to achieve the effect of easy biological activity, easy maintenance, and mild preparation process conditions

Active Publication Date: 2016-01-06
于炜婷
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantages of this method are as follows: the introduction of cross-linking agent requires secondary emulsification, which is prone to demulsification and greatly reduces the yield of microspheres; the introduction of organic phase and acetic acid solution in the preparation process is likely to affect the enzyme activity; a large amount of oily substances are introduced in the preparation process, thus limiting The scope of application of the product is limited; the thrombin is embedded by adsorption method, which leads to the limited drug loading of thrombin. After freeze-drying, the highest drug loading in the product is only 23%, and the highest encapsulation rate is 61%.
Since the solid matter in the calcium alginate hydrogel microspheres is sodium alginate except for the enzyme, the mass ratio of the enzyme to sodium alginate in the products of the disclosed technology is up to 1:3.3, and the drug loading is too low. Large impact on the efficacy of microspheres

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] 【1】Preparation of sodium alginate solution (concentration 15g / L);

[0026] [2] Dissolve thrombin (100 mg) into the sodium alginate solution prepared in step [1] at a concentration of 10 g / L to obtain a sodium alginate solution containing thrombin;

[0027] [3] Prepare the gel bath solution, which contains calcium chloride (concentration 30g / L), sodium chloride (concentration 5g / L), sodium dihydrogen phosphate (concentration 1g / L), Tween 5g / L in the gel bath ;

[0028] [4] Under the high-voltage electrostatic field, the sodium alginate solution containing thrombin prepared in step [2] is formed into uniform jet droplets, and the droplets enter the gel bath solution prepared in step [3] to gel. Reaction, reacting for 30 minutes to obtain alginate hydrogel microspheres embedded with thrombin, the particle size of the microspheres is 500±100 microns.

[0029] [5] The thrombin-embedded alginate hydrogel microspheres prepared in step [4] were reacted with chitosan solution ...

Embodiment 2

[0034] 【1】Preparation of sodium alginate solution (concentration 20g / L);

[0035] [2] Dissolve hemocoagulase (50 mg) into the sodium alginate solution prepared in step [1] at a concentration of 20 g / L to obtain a sodium alginate solution containing hemagglutinin;

[0036] [3] Prepare the gel bath solution, which contains barium chloride (concentration 20g / L), sodium chloride (concentration 1g / L), sodium dihydrogen phosphate (concentration 1g / L), Tween 10g / L in the gel bath ;

[0037] [4] Under a high-voltage electrostatic field, the sodium alginate solution containing hemocoagulase prepared in step 2 is formed into uniform jet droplets, and the droplets enter the gel bath solution prepared in step [3] to undergo a gelation reaction After reacting for 30 minutes, the alginate hydrogel microspheres embedded with hemocoagulase were obtained, and the particle size of the microspheres was 300±100 microns.

[0038] [5] Divide the hemocoagulase-embedded alginate hydrogel microspher...

Embodiment 3

[0040] 【1】Preparation of sodium alginate solution (concentration 15g / L);

[0041] [2] Dissolve thrombin (200mg) into the sodium alginate solution prepared in step [1] at a concentration of 20g / L to obtain a sodium alginate solution containing thrombin;

[0042] [3] Prepare the gel bath solution, which contains calcium chloride (concentration 25g / L), sodium chloride (concentration 3g / L), F68 (5g / L) in the gel bath;

[0043] [4] Under the high-voltage electrostatic field, the sodium alginate solution containing thrombin prepared in step [2] is formed into uniform jet droplets, and the droplets enter the gel bath solution prepared in step [3] to gel. Reaction, reacting for 30 minutes to obtain alginate hydrogel microspheres embedded with thrombin, the particle size of the microspheres is 500±100 microns.

[0044] [5] Take a certain volume of the microspheres prepared in step [4], and refer to the literature (Research on the chemical capsule breaking method of cell-loaded sodium ...

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Abstract

The invention discloses hydrogel microspheres embedded with coagulation related enzyme and a preparation process of the hydrogel microspheres. The hydrogel microspheres and the preparation process have the advantages that the particle size of the prepared hydrogel microspheres is 100-1000 micrometers and is uniform (CV<20%), the coagulation related enzyme is evenly distributed in the hydrogel microspheres, the mass ratio of the coagulation related enzyme to sodium alginate is 1:3-4:1, and the drug loading capacity of the coagulation related enzyme after dewatering is 25-80%.

Description

technical field [0001] The invention relates to a hydrogel microsphere, in particular to a hydrogel microsphere embedded with coagulation-related enzymes. Background technique [0002] The currently reported microspheres embedded with coagulation-related enzymes are all dry microparticle products. The preparation method adopts the emulsification-crosslinking method to prepare microspheres first, and then absorb thrombin on the microspheres and then dry them to prepare thrombin-embedded microspheres. (Reference: Preparation and Stability Study of Sodium Alginate_Chitosan Immobilized Thrombin Microspheres, PLA Pharmaceutical Journal, 2012, Volume 28, Issue 4). The disadvantages of this method are as follows: the introduction of cross-linking agent requires secondary emulsification, which is prone to demulsification and greatly reduces the yield of microspheres; the introduction of organic phase and acetic acid solution in the preparation process is likely to affect the enzyme ...

Claims

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Application Information

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IPC IPC(8): A61K9/16A61K38/48A61K47/36B01J13/02
Inventor 马小军韩雅玲于炜婷梁明郑会珍荣晶晶
Owner 于炜婷
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