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Alginate-based-polycationic microcapsule and applications thereof in embedding bioactive substance

A bioactive substance, alginate technology, applied in the field of alginate microcapsule products, can solve the problems of inappropriate active substance embedding, long time-consuming, complicated microcapsule preparation methods, etc. Gentle and controllable, high stability effect

Inactive Publication Date: 2017-06-20
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the preparation method of the microcapsules involved is complex and time-consuming, and the chemical molecules and small solvent molecules used have potential cytotoxicity, which is not suitable for the embedding of active substances.

Method used

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  • Alginate-based-polycationic microcapsule and applications thereof in embedding bioactive substance
  • Alginate-based-polycationic microcapsule and applications thereof in embedding bioactive substance
  • Alginate-based-polycationic microcapsule and applications thereof in embedding bioactive substance

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] 1) Prepare sodium alginate solution: dissolve 2 g of sodium alginate in 200 ml of deionized water, add 2.132 g of MES, and adjust the pH value to about 5.5. Sodium alginate has a molecular weight of 320kDa and a G / M ratio of 55 / 45.

[0037] 2) Filter 1) solution to remove impurities.

[0038] 3) Add 1.936 g of water-soluble carbodiimide (EDC·HCl) and 1.116 g of N-hydroxysuccinimide (NHS) into the sodium alginate solution in step 2) and activate for 40 minutes.

[0039] 4) Add 1.92 g of dopamine hydrochloride as a solid to the sodium alginate solution in step 3), and react at room temperature for 12 hours under nitrogen protection.

[0040] 5) Add 400ml of ethanol dropwise to the sodium alginate solution in step 4) to obtain a precipitated sodium alginate solid. After washing with ethanol three times, the product was collected.

[0041] 6) The product obtained in step 5) was vacuum-dried to obtain dopamine-grafted sodium alginate with a grafting degree of 26%.

[004...

Embodiment 2

[0059] 1) Prepare sodium alginate solution: dissolve 2 g of sodium alginate in 200 ml of deionized water, add 2.132 g of MES, and adjust the pH value to about 5.5. Sodium alginate has a molecular weight of 320kDa and a G / M ratio of 55 / 45.

[0060] 2) Filter 1) solution to remove impurities.

[0061] 3) Add 1.936 g of EDC·HCl and 1.116 g of solid NHS into the sodium alginate solution in step 2), and activate for 40 minutes.

[0062] 4) Add 1.92 g of dopamine hydrochloride as a solid to the sodium alginate solution in step 3), and react at room temperature for 12 hours under nitrogen protection.

[0063] 5) Add 400ml of ethanol dropwise to the sodium alginate solution in step 4) to obtain a precipitated sodium alginate solid. After washing with ethanol three times, the product was collected.

[0064] 6) The product obtained in step 5) was vacuum-dried to obtain dopamine-grafted sodium alginate with a grafting degree of 26%.

[0065] 7) Dissolving the dopamine-grafted sodium ...

Embodiment 3

[0080] 1) Preparation of semi-dopamine-modified sodium alginate solution: dopamine-alginic acid was dissolved in physiological saline to prepare a 15 g / L galactosyl sodium alginate solution, which was sterile filtered. Among them, the dopamine-modified alginic acid has a molecular weight of 350kDa.

[0081] 2) Preparation of α-polylysine solution: α-polylysine was dissolved in physiological saline to prepare a 5 g / L α-polylysine solution, which was sterile filtered. Among them, the molecular weight of α-polylysine is 30kDa.

[0082] 3) Preparation of gel bath solution: Anhydrous calcium chloride was dissolved in deionized water to prepare a 11 g / L calcium chloride solution, which was sterilized by filtration.

[0083] 4) Preparation of dopamine-modified calcium alginate / α-polylysine-embedded hydrogel microsphere carrier wrapped pig liver cells: take 3mL dopamine-modified sodium alginate solution, add 6*10^6 rat primary Hepatocytes were mixed evenly, and hydrogel microspheres...

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Abstract

The invention relates to an alginate-polycationic microcapsule product used for embedding bioactive substance, in particular to an alginate-polycationic microcapsule subjected to graft modification of dopamine. According to the alginate-polycationic microcapsule subjected to graft modification of dopamine, dopamine molecules are in covalent bonding to sodium alginate molecules, the obtained molecules are used for preparing a hydrogel microsphere carrier, the embedded hydrogel microsphere carrier further has film formation reaction with polycations, and thus the dopamine-based alginate / polycationic microcapsule is prepared. The microcapsule has good stability, and does not swell or break in the body fluid environment. The product is mainly used for embedding the bioactive substance, the microcapsule film has the excellent biocompatibility while having the good film strength, and the integrity of the microcapsule film when taken as the histocyte transplantation carrier, the cell carrier of the in-vitro bioartificial liver system, the cell carrier in the cell culture process and the like in the application process is guaranteed.

Description

technical field [0001] The present invention designs a kind of alginate microcapsule product that is used for bioactive substance embedding, specifically the preparation of a dopamine graft-modified alginate-polycation microcapsule and its embedding in bioactive substance in the application. Background technique [0002] Materials and preparation methods related to microencapsulation technology are currently a research hotspot, and have been widely used in preclinical research in biomedical fields such as cell transplantation, drug release, and gene therapy (Wang et al., 2006). Among the numerous biological microcapsules, especially in the field of microcapsules for embedding biologically active substances, alginate-polycation microcapsules are widely used. Alginate-polycation microcapsules include sodium alginate-α-polylysine (alginate / α-polylysine) microcapsules (abbreviated as α-APA microcapsules), sodium alginate-ε-polylysine (alginate / ε-polylysine) microcapsules (ref...

Claims

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Application Information

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IPC IPC(8): A61K9/50A61K45/00A61K47/18C08B37/04
CPCA61K9/5036A61K45/00A61K47/18C08B37/0084
Inventor 谢红国任英于炜婷娄茹云刘晓岑马小军
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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