Animal cloned embryo culture solution and method

Abstract

The invention discloses an animal cloned embryo culture solution and method. The method comprises the steps that tauroursodeoxycholic acid is added into an embryo basal culture solution according to 50-100 microns to obtain the embryo culture solution; animal cloned embryos obtained through nuclear transplantation are transferred into the embryo culture solution for culture. By means of the animalcloned embryo culture solution and method, tauroursodeoxycholic acid is utilized for significantly inhibiting endoplasmic reticulum stress of the animal cloned embryos in the culture stage after thenuclear transplantation, and meanwhile, the in-vitro development rate of the animal cloned embryos is significantly increased. The animal cloned embryo culture solution and method improve the in-vitroculture efficiency of the animal cloned embryos, and have important application value especially in obtaining genetically-modified rabbits and cloned rabbits.

Description

technical field [0001] The invention relates to in vitro embryo culture, in particular to animal cloning embryo culture solution and culture method. Background technique [0002] Nuclear transfer is a cell engineering technique in which a donor cell nucleus is transferred into an enucleated oocyte and a reconstructed embryo is obtained. This technology is of great value in the fields of constructing genetically modified animals, saving endangered species, stem cell therapy, and mitochondrial therapy. Currently, the efficiency of somatic cell nuclear transfer is extremely low. [0003] Reconstructed embryos obtained by nuclear transfer are different from fertilized embryos. The process of constructing embryos involves micromanipulation processes (enucleation, nuclear injection, fusion and activation), and the stimulation of nuclear transfer operations can induce cells to undergo endoplasmic reticulum stress. Excited. Stimulation of in vitro culture conditions can also indu...

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Problems solved by technology

[0005] During somatic cell nuclear transfer, operations such as enucleation, fusion, activation, and embryo culture will cause endoplasmic reticulum stress and oxidative stress in animal cloned embryos. At present, there is no in vitro culture medium specifically for animal cloned embryos, especially in In the culture of cloned embryos of gene-edited rabbits and somatic cell nuclear transfer rabbits, it is urgent to propose a culture medium and culture method that can inhibit the endoplasmic reticulum stress of cloned embryogenesis and improve the development rate of animal cloned embryos

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