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Goat cell clone embryo culture solution and culture method

A technology for cloning embryos and culture medium, applied in the field of bioengineering, can solve the problems of low development rate of cloned embryos, and achieve highly targeted effects

Active Publication Date: 2017-09-26
FUYANG NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The invention provides a goat cell cloning embryo culture solution, which is highly targeted and solves the problem that the development rate of cloned embryos generally required by M16 medium is relatively low

Method used

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  • Goat cell clone embryo culture solution and culture method

Examples

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Effect test

Embodiment 1

[0021] A kind of goat cell clone embryo culture liquid, comprises A liquid and B liquid, contains the ferrous nitrate of 0.2mg, the potassium chloride of 350mg, the citric acid of 100mg, the sodium dihydrogen phosphate of 100mg, the remaining The amount is DMEM high-sugar liquid medium;

[0022] Each liter of the liquid B contains 15 mg of fetal bovine serum, 150 mg of malic acid, 0.2 g of sodium chloride, and the balance is M16 liquid medium.

[0023] The malic acid is L-malic acid.

Embodiment 2

[0025] A kind of goat cell clone embryo culture liquid, comprises A liquid and B liquid, contains the ferrous nitrate of 0.2mg, the potassium chloride of 350mg, the citric acid of 120mg, the sodium dihydrogen phosphate of 100mg, the remaining The amount is DMEM high-sugar liquid medium;

[0026] Each liter of the liquid B contains 17 mg of fetal bovine serum, 120 mg of malic acid, 0.2 g of sodium chloride, and the balance is M16 liquid medium.

[0027] The malic acid is D-malic acid.

Embodiment 3

[0029] A kind of goat cell clone embryo culture liquid, comprises A liquid and B liquid, contains the ferrous nitrate of 0.2mg, the potassium chloride of 350mg, the citric acid of 100mg, the sodium dihydrogen phosphate of 110mg, the remaining The amount is DMEM high-sugar liquid medium;

[0030] Each liter of the liquid B contains 10 mg of fetal bovine serum, 130 mg of malic acid, 0.2 g of sodium chloride, and the balance is M16 liquid medium.

[0031] The malic acid is the mixture DL-malic acid.

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Abstract

The invention belongs to the technical field of biological engineering, and discloses a goat cell clone embryo culture solution and a culture method thereof. The goat cell clone embryo culture solution comprises a solution A and a solution B, wherein per liter of liquid A contains 0.2mg of ferrous nitrate, 350mg of potassium chloride, 100 to 120mg of citric acid, 100mg of monosodium orthophosphate and the balance DMEM high-sugar liquid culture media. The culture solution is characterized in that a DMEM culture medium and an M16 culture medium in the prior art are improved; the liquid A and the liquid B of the culture solution are prepared; then, the cells are cultured in different stages; the two-cell embryo number, the four-cell embryo number, the mulberry embryo number, the blastaea number and the survival number after the blastaea implantation into a matrix are high; the in vitro development rate of the goat fertilized ova are higher than those of DMEM culture media and M16 culture media.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a goat cell cloned embryo culture solution and a culture method. Background technique [0002] Goats are an important economic animal that produces meat, velvet, milk and other economic products, especially goat milk, which has huge market potential. If high-quality genetic resources of goat breeds are obtained and maintained, it is necessary to improve the existing population and become a goat The urgent problem of large-scale farming. [0003] Embryo cloning is a method of cloning, the most commonly used is somatic cell cloning technology, which refers to the method of nuclear transfer, cell disassembly or cell recombination technology, the enucleation of oocytes as nuclear receptors, somatic cells or The nuclear plastid containing a small amount of cytoplasm is used as the nuclear donor, and the nuclear donor is transferred into the nuclear recipient to con...

Claims

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Application Information

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IPC IPC(8): C12N5/10
CPCC12N5/0604C12N2500/12C12N2500/24C12N2500/30C12N2510/00
Inventor 刘勇李文雍吴晓庆谢娟章孝荣凌英会鞠明
Owner FUYANG NORMAL UNIVERSITY
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