Inducing medium and inducing method for inducing multipotential stem cells to form mesoblastic cells

Abstract

The invention provides an inducing medium for inducing multipotential stem cells to form mesoblastic cells. The inducing medium is prepared from the following components with the corresponding concentrations: 5-15 mM of LiCl, 0.5-2 % of glutamine, 0.5-2 % of non-essential amino acid, 5-15 [mu] M of CHIR99021, 8-15 ng / ml of bFGF, 0.05-0.2 mmol / l of beta mercaptoethanol, 0.2-0.6 [mu] g / ml of hydrocortisone, 4-10 [mu] g / ml of insulin, 80-120 [mu] g / ml of transferrin, 8-12 ng / ml of sodium selenite, 0.05-0.2 mg / ml of penicillin, and 0.05-0.2 mg / ml of streptomycin. For the inducing medium, on the basis of the DMEM / F12 culture medium, some specific cytokines and supplements are added, and thus the probability of inducing the multipotential stem cells to differentiate towards the mesoblastic cells is improved. In addition, the invention provides an inducing method for inducing the multipotential stem cells to form the mesoblastic cells, according to the method, the simple embryoid body for inducing the multipotential stem cells is inoculated to the inducing medium for induced culture, and thus the multipotential stem cells efficiently differentiate to form the mesoblastic cells.

Description

technical field [0001] The invention relates to an induction medium and an induction method, in particular to an induction medium and an induction method for inducing pluripotent stem cells to form mesoderm cells. Background technique [0002] Skin tissue engineering has been a research hotspot in recent years, but skin-related cells are all terminally differentiated cells, and it is difficult to carry out large-scale culture and expansion in vitro. The source of seed cells hinders the development of tissue engineering. Autologous seed cells Sources are limited, allogeneic seed cells have rejection reactions, and stem cells have ethical issues. Therefore, urine cells are collected from urine, and urine cells are induced to become IPS cells, and ips cells are then induced to become mesoderm cells, fibroblasts, and sweat glands Cells, mesoderm cells, etc. can solve the problem of the source of seeds for skin tissue engineering. Cells can be obtained from urine, which is conven...

AI Technical Summary

Problems solved by technology

However, the induction efficiency of simple cytokines is low, the co-culture induction has immunogenicity, and the embryoid body has the characteristics of non-directional induction, and finally forms three germ layers, and cannot efficiently obtain a certain germ layer. Therefore, the present invention provides a high-efficiency induction of pluripotency Method for differentiation of stem cells into mesoderm cells

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