Egg yolk antibody used for preventing and treating Muscovy duck source gosling plague

A yolk antibody, gosling plague virus technology, applied in the direction of antibodies, microorganism-based methods, antiviral agents, etc., can solve the problems of virus spread, harm, parvovirus differences, etc.

Active Publication Date: 2016-09-21
YEBIO BIOENG OF QINGDAO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disease first appeared in China and many European countries in the middle and late 1960s. It was not called goose parvovirus infection until 1978. The isolated parvoviruses were significantly different
If the dead Muscovy ducks are not handled properly, the virus will often spread and cause greater harm.
At present, there is only live vaccine against parvovirus disease of muscovy ducks on the market, and there is no live vaccine for parvovirus disease of muscovy ducks and goose, and there is no fast and effective drug or method to prevent and treat parvovirus disease of muscovy ducks and geese

Method used

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  • Egg yolk antibody used for preventing and treating Muscovy duck source gosling plague
  • Egg yolk antibody used for preventing and treating Muscovy duck source gosling plague
  • Egg yolk antibody used for preventing and treating Muscovy duck source gosling plague

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Embodiment 1, the screening of YBGPV-M strain

[0014] In 2013, a disease characterized by diarrhea and embolism due to the shedding of some duck intestinal mucosa appeared in a group of young muscovy ducks in a duck farm in Fujian Province. The incidence rate was 50% to 80%, and the fatality rate was 45% to 70%. The use of antibiotics, Muscovy duck parvovirus vaccine and Muscovy duck parvovirus hyperimmune yolk antibody could not control the disease. The inventor aseptically collected the liver, spleen and pancreas of dying ducks, homogenized the liver, spleen and pancreas with sterile physiological saline to make a 20% suspension, centrifuged at 3000r / min for 15min, took the bacteria from them and passed through the allantoic cavity Inoculate 11-day-old muscovy duck embryos, hatch for 168 hours, collect the allantoic fluid and embryo body tissue of the dead embryos after 24 hours, homogenize, freeze and thaw three times, and take the supernatant for freezing. After t...

Embodiment 2

[0017] Embodiment 2, the preparation of vaccine

[0018] 1. Preparation of virus liquid for seedling production: Dilute the virus seed YBGPV-M strain 100 times with sterile saline, inoculate 11-12 day-old susceptible Muscovy duck embryos into the allantoic cavity, 0.2ml per embryo, and incubate at 37°C. Embryo inspections were performed twice a day. Select duck embryos that died within 48 to 168 hours after inoculation, place them at 2 to 8°C for 4 to 12 hours, open the air chamber under sterile conditions, and pick out embryos with extensive hemorrhage, hair follicle hemorrhage, liver degeneration and necrosis, and chorioallantois For embryos with mild edema, take the embryo body (remove the head and limbs) and put it into a tissue grinder to pulverize, collect allantoic fluid and amniotic fluid, homogenize with embryo fluid, take the pulverized tissue and add it in a ratio of 1:3 Physiological saline was mixed, freeze-thawed 3 times, centrifuged at 4000r / min for 30min, the ...

Embodiment 3

[0047] Example 3: Preparation of Egg Yolk Antibody to Gosling Plague Virus

[0048] 1. Preparation of Vaccine Immune Eggs

[0049] Immune laying hens with the inactivated gosling plague vaccine prepared above, and inject 1 portion of muscovy duck-derived gosling plague inactivated vaccine subcutaneously into the neck of each chicken for the first immunization, and perform the second immunization 14 days later, subcutaneously inject the neck of each chicken Inject 2.0ml of inactivated vaccine, 14 days after the second immunization for the third immunization, each chicken neck subcutaneous injection of 2.0ml inactivated vaccine, 14 days after the third immunization for the fourth immunization, each chicken neck subcutaneous injection of 2.0 ml of inactivated vaccine, 14 days after the fourth immunization, the egg yolk was collected to determine the AGP antibody titer of gosling plague virus should not be lower than 1:64.

[0050] 2 Yolk antibody production (see Table 4)

[005...

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Abstract

The invention provides an egg yolk antibody used for preventing and treating Muscovy duck source gosling plague. The egg yolk antibody is prepared with a Muscovy duck source gosling plague virus with the preservation serial number of CCTCC NO: V201620 as an antigen. Muscovy duck embryos are inoculated with the Muscovy duck source gosling plague virus strain YBGPV-M, idiosome and blastochyle of dead embryos are obtained respectively, virus liquid is obtained after grinding and freeze thawing, an oil adjuvant is added for mixing emulsification after ultrafiltration concentration and formaldehyde solution inactivation, and a vaccine is obtained; laying hens are inoculated with the vaccine, eggs are obtained, yolks are separated, inactivation, extraction and refining are carried out, and the antibody is obtained. The prepared antibody can prevent young Muscovy duck gosling plague virus infection caused by the gosling plague virus; the egg yolk antibody has the advantages of being efficient, good in safety, high in protection rate and the like.

Description

technical field [0001] The invention belongs to the field of veterinary biological products, and in particular relates to an egg yolk antibody for preventing and treating muscovy duck-derived gosling plague. Background technique [0002] Goose parvovirus disease, also known as Derzsy's disease, commonly known as goose flu, goose or gosling plague, foie hepatitis, goose enteritis, etc., is a highly contagious disease that affects young geese and Muscovy ducks. The diversity of disease names reflects multiple pathological features of the disease. According to the age of infected goslings and ducklings, the disease can be manifested as acute, subacute and chronic. The acute type can cause 100% death of goslings within 10 days of age. Many countries have also reported a Muscovy duck parvovirus infection with a completely different antigenicity, with a mortality rate as high as 80%. The disease mainly occurs in 1 to 3-week-old gooses and young Muscovy ducks, especially those a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/08C07K16/02C12N7/00A61K39/42A61P31/20C12R1/93
CPCA61K2039/505A61K2039/552C07K16/02C07K16/081C07K2317/11C12N7/00C12N2750/14321Y02A50/30
Inventor 邹敏吴发兴韩乃君范根成杜元钊郭莉莉陶晓珊
Owner YEBIO BIOENG OF QINGDAO
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