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Chick embryo allantoic cavity inoculation method for increasing avian influenza virus separation rate

An avian influenza virus, chicken embryo allantoic technology, applied in the direction of virus/phage, biochemical equipment and methods, microorganisms, etc., can solve the problem of inoculation volume not exceeding 0.2ml, liquid overflowing dead embryos, etc.

Inactive Publication Date: 2008-06-04
WUHAN INST OF VIROLOGY CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the long-term application process, this inoculation method has the defect that the inoculation volume cannot exceed 0.2ml. When the inoculation volume is greater than or equal to (≥) 0.3ml / embryo, there will be liquid overflow (increased internal pressure) and dead embryos will increase within 24 hours.

Method used

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  • Chick embryo allantoic cavity inoculation method for increasing avian influenza virus separation rate
  • Chick embryo allantoic cavity inoculation method for increasing avian influenza virus separation rate
  • Chick embryo allantoic cavity inoculation method for increasing avian influenza virus separation rate

Examples

Experimental program
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Effect test

Embodiment 1

[0010] Adopt traditional chicken embryo allantoic inoculation method and chicken embryo allantoic inoculation method of the present invention, carry out avian influenza virus isolation rate comparison (operating method is respectively as follows figure 1 , as shown in 2). Two methods were used for the same sample at the same time, and one chicken embryo was inoculated respectively.

[0011] The samples were 82 duck samples suspected of avian influenza in a county in Hubei in 2005. The specific operation method is as figure 2 Shown: 82 chicken embryos hatched at 9-11 days old were used, and the position of the air chamber and the embryo was marked out after inspection, and a pinhole (decompression hole) was made on the side of the air chamber; the embryo position was placed horizontally On the shelf, avoid the embryo body 0.3-0.5cm to make a pinhole (into the needle hole), directly insert the needle into the allantoic cavity to inoculate the sample 0.5-1.0ml / embryo, and seal...

Embodiment 2

[0022] Adopt traditional chicken embryo allantoic inoculation method and chicken embryo allantoic inoculation method of the present invention, carry out avian influenza virus isolation rate comparison (operating method is respectively as follows figure 1 , as shown in 2). Two methods were used for the same sample at the same time, and one chicken embryo was inoculated respectively.

[0023] The samples were 69 chicken samples suspected of avian influenza in a county in Zhejiang in 2005. Each sample was inoculated with one chicken embryo by two methods at the same time. The specific operation method is as figure 2 Shown: 69 chicken embryos hatched at 9-11 days old were used, the air chamber and the position of the embryo were marked out after inspection, and a pinhole (decompression hole) was made on the side of the air chamber; the embryo position was placed horizontally On the shelf, avoid the embryo body 0.3-0.5cm to make a pinhole (into the needle hole), directly insert...

Embodiment 3

[0029] Adopt traditional chicken embryo allantoic inoculation method and chicken embryo allantoic inoculation method of the present invention, carry out avian influenza virus isolation rate comparison (operating method is respectively as follows figure 1 , as shown in 2). Two methods were used for the same sample at the same time, and one chicken embryo was inoculated respectively.

[0030] The samples were 55 chicken samples suspected of avian influenza in a county of Hunan Province in 2004. Specific operations such as figure 2 As shown, 55 chicken embryos hatched at the age of 9-11 days were used, and the position of the air chamber and the embryo was marked out after inspection, and a pinhole (decompression hole) was made on the side of the air chamber; the position of the embryo was placed horizontally. On the shelf, avoid the embryo body 0.3-0.5cm to make a pinhole (into the needle hole), directly insert the needle into the allantoic cavity to inoculate the sample 0.5-...

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Abstract

The invention relates to a chick embryo allantoic cavity inoculation method for improving separation rate of bird flu viruses. The invention is characterized in that: a pinhole is arranged on the side of an air chamber of a chick embryo and taken as a pressure-relief vent; a pinhole is arranged on an embryo body of the chick embryo close to an allantoic cavity and taken as a needle insertion hole; a needle is directly inserted into the allantoic cavity for inoculation of sample by 0.5 to 1.0 milliliter per embryo. The invention reaches the goal of increasing viral load by increasing inoculum concentration, thereby the separation rate of viruses is improved.

Description

Technical field: [0001] The invention relates to virus isolation and inoculation technology, and in particular provides a chicken embryo allantoic cavity inoculation method for improving the isolation rate of avian influenza virus. Background technique: [0002] Avian influenza virus is an important source of respiratory diseases in poultry and some mammals. It is an acute and severe infectious disease caused by a specific subtype of Orthomyxoviridae A virus, which seriously endangers the poultry industry and human health. The variation of avian influenza virus is frequent, and there are many serotypes, including H1-H15, N1-9, which infect almost all birds. Isolating and obtaining the pathogenic pathogen (virus) from the sample is an important basis for diagnosing viral infectious diseases (bird flu). Isolation of avian influenza strains is generally carried out by collecting poultry (chicken, duck, etc.) cloacal cotton swabs at present, and then usually adopting chicken em...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/00
Inventor 李天宪唐霜陈绳亮张忠李永东寇铮范兆军
Owner WUHAN INST OF VIROLOGY CHINESE ACADEMY OF SCI
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