Method for preparing infectious chicken Fabricius bursa refined yolk cryodesiccation antibody

Abstract

A preparation method of a purified vitelline freezing antibody for chickens infectious bursal disease is characterized in that: high purity antigens are adopted to vaccinate IBDV B87 to SPF chickens, then velvet urine, idiosome, velvet urine membrence are acquired, grinded, filtered and inactivated to antigens, and the steps are: producing immune eggs, collecting high immune egg, separating vitelline, diluting with distilled water, inactivating, extraction, decentering, inactivating, freezing and checking; the eggs is radiated by Co60 to kill viruses and bacterium, and finally froze by novel immuno-enhancer - Astragali Polysaccharoses and preserved under a temperature of 2-8 DEG C. Composite drugs for prevention and therapy are prepared by adopting the vitelline antibody for chickens infectious bursal disease as major components and cooperating with a novel immuno-enhancer, to decrease the jeopardy of the chicken breeding industry, and improve the living level of people and benefit mankind.

Description

Technical field:

[0001] The invention relates to the technical field of poultry biopharmaceutical preparations, and relates to a method for preparing a chicken infectious bursa purified egg yolk freeze-dried antibody. Background technique:

[0002] Chicken bursal disease is an acute and highly contagious infectious disease caused by young chickens. It has a short course of disease, high morbidity and mortality. The virus has the greatest damage to the bursa of the chicken body, and is one of the most serious infectious diseases in the world's broiler farming industry. After young chickens are infected, it can lead to immunosuppression, and can induce various diseases or make various vaccines fail to immunize, thereby causing disease outbreaks and epidemics, with a mortality rate ranging from 20% to 50%. The disease can inhibit the humoral immunity and cellular immunity of chickens, and can reduce the responsiveness of chickens to vaccination. Young chickens suffer from syst...