Method for preparing infectious chicken Fabricius bursa refined yolk cryodesiccation antibody

A technology of chicken infectivity and bursa, which is applied in the direction of antibodies, antiviral agents, and immunoglobulin from eggs, etc., can solve the problems of infectious diseases and bursa damage in broiler breeding industry, and prevent immunosuppressive diseases Occurrence, easy storage, high purity effect

Inactive Publication Date: 2008-01-30
PU LIKE BIO ENG
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AI Technical Summary

Problems solved by technology

[0005] In order to solve the problem that poultry viruses have the greatest damage to the bursa of chicken body and cause the most serious infectious disease in the broiler breeding industry, the purpose of the present invention is to provide a method for preparing chicken infectious bursa purified egg yolk freeze-dried antibody, The prevention and treatment compound drug made with French sac egg yolk antibody as the main ingredient and a new type of immune enhancer can reduce the harm in the broiler breeding industry, benefit human beings, and improve people's living standards

Method used

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specific Embodiment approach 3

[0018] Preparation of virus seeds for production: IBDV B87 strain virus seeds are properly diluted, inoculated, and harvested; virus propagation: IBDV B87 strain virus seeds are properly diluted, inoculated, harvested embryo fluid, embryo body chorioallantoic membrane, ground and filtered; inactivated antigen: Immune laying hens, collect high-free eggs, take 17 grams of high-free egg yolks, dilute with distilled water; inactivation: heat inactivation at 60°C for 8-15 minutes, extract, centrifuge, add ammonium sulfate with a final concentration of 15-18%, 8000 Spin / centrifuge for 15 minutes, collect the precipitate; inactivation: cobalt 60 irradiation to kill exogenous virus in egg yolk, add astragalus polysaccharide 5g, polymyxin B2 micrograms, sorbitol, mannitol protective agent to the semi-finished product, pack and freeze Drying, finished product inspection, labeling, packaging and storage.

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Abstract

A preparation method of a purified vitelline freezing antibody for chickens infectious bursal disease is characterized in that: high purity antigens are adopted to vaccinate IBDV B87 to SPF chickens, then velvet urine, idiosome, velvet urine membrence are acquired, grinded, filtered and inactivated to antigens, and the steps are: producing immune eggs, collecting high immune egg, separating vitelline, diluting with distilled water, inactivating, extraction, decentering, inactivating, freezing and checking; the eggs is radiated by Co60 to kill viruses and bacterium, and finally froze by novel immuno-enhancer - Astragali Polysaccharoses and preserved under a temperature of 2-8 DEG C. Composite drugs for prevention and therapy are prepared by adopting the vitelline antibody for chickens infectious bursal disease as major components and cooperating with a novel immuno-enhancer, to decrease the jeopardy of the chicken breeding industry, and improve the living level of people and benefit mankind.

Description

Technical field: [0001] The invention relates to the technical field of poultry biopharmaceutical preparations, and relates to a method for preparing a chicken infectious bursa purified egg yolk freeze-dried antibody. Background technique: [0002] Chicken bursal disease is an acute and highly contagious infectious disease caused by young chickens. It has a short course of disease, high morbidity and mortality. The virus has the greatest damage to the bursa of the chicken body, and is one of the most serious infectious diseases in the world's broiler farming industry. After young chickens are infected, it can lead to immunosuppression, and can induce various diseases or make various vaccines fail to immunize, thereby causing disease outbreaks and epidemics, with a mortality rate ranging from 20% to 50%. The disease can inhibit the humoral immunity and cellular immunity of chickens, and can reduce the responsiveness of chickens to vaccination. Young chickens suffer from syst...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/02A61K39/42A61P31/12
Inventor 张许科孙进忠乔荣岑丁梅闵亚杰孟照国习向峰
Owner PU LIKE BIO ENG
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